Invitrogen

CD3 Monoclonal Antibody (OKT3), FITC, eBioscience™

FIGURE: 1 / 62

CD3 Antibody (11-0037-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD3 FITC (right). Cells in the lymphocyte gate were used for analysis.

CD3 Antibody in Immunocytochemistry (ICC/IF)

CD3 Antibody in Immunohistochemistry (IHC)

CD3 Antibody in Functional Assay (Functional)

Product Image Gallery

CD3 Antibody (11-0037-42) in — Figure 5--figure supplement 2. F-actin foci on microdots are not enriched in phospholipid membranes. ( A ) F-actin enrichment at the microdot is not a consequence of cell morphology around the microdot. Human CD4 T cells were labeled with CM-DiI according to manufacturer's protocol and were then incubated with glass coverslip coated with patterned Alexa647 tagged anti-CD3 and ICAM1 for 5 min, fixed and stained with Alexa488-phalloidin. Cells were then imaged using TIRF microscopy. The graphs on the bottom panels show line-scan profiles of anti-CD3 (TCR, red, left), actin (Green, center) and DiI (blue, right) acquired from 70 different microdots. The TCR, actin and DiI intensities were measured from identical pixel positions for a given microdot, were then normalized by the lowest pixel intensity per microdot for a given fluorescent channel, and plotted. Note that while actin shows enrichment at the microdot site, DiI is not enriched at the same position. ( B ) The same procedure as described above, except with the use of Cy3-anti- LFA1 Fab fragments instead of DiI, was carried out in T cells, and the normalized line-scan intensities were plotted as average value per pixel position +-SEM. The graph represents mean of normalized intensities across 25 pixels, acquired using 74 different microdots. In both these experiments ( A , B ), n > 20. DOI: http://dx.doi.org/

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone OKT3 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone OKT3 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone OKT3) and CD19 (clone HIB19). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used to compare CD3 staining (middle plot). Cells in the lymphocyte gate were used to compare CD3 and CD19 staining (right plot). {RE}

Product Details

11-0037-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 45 publications 45 publications

Functional Assay (Functional)

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 5 publications 5 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT3

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2016669

Product Specific Information

Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Antibody clones OKT3 and SK7 see different epitopes.

Applications Reported: The OKT3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Endosialin-positive tumor-derived pericytes promote tumor progression through impeding the infiltration of CD8⁺ T cells in clear cell renal cell carcinoma.

Cancer immunology, immunotherapy : CII

Lu T,Zhang J,Lu S,Yang F,Gan L,Wu X,Song H,Liu S,Xu C,Han D,Yang B,Wen W,Qin W,Yang L

11-0037-42 was used in Flow cytometry/Cell sorting to analyzed the relationship between endosialin (EN) expression and cytotoxic T-lymphocyte.

Thu Jun 01 00:00:00 UTC 2023

Single-cell sequencing of ascites fluid illustrates heterogeneity and therapy-induced evolution during gastric cancer peritoneal metastasis.

Nature communications

Huang XZ,Pang MJ,Li JY,Chen HY,Sun JX,Song YX,Ni HJ,Ye SY,Bai S,Li TH,Wang XY,Lu JY,Yang JJ,Sun X,Mills JC,Miao ZF,Wang ZN

11-0037-42 was used in Flow cytometry/Cell sorting to describe different cell developmental lineage trajectories in the peritoneal ecosystem and provide evidence to better understand the mechanism of GCPM, aiding exploration of effective target strategies for GCPM therapy.

Tue Feb 14 00:00:00 UTC 2023

Plasmid-Based Donor Templates for Nonviral CRISPR/Cas9-Mediated Gene Knock-In in Human T Cells.

Current protocols

Senger K,Akhmetzyanova I,Haley B,Rutz S,Oh SA

11-0037-42 was used in Flow Cytometry /test) to report a simple and efficient method for nonviral CRISPR/Cas9-based gene knock-in utilizing plasmid-based donor DNA templates.

Thu Sep 01 00:00:00 UTC 2022

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

Agudelo M,Palus M,Keeffe JR,Bianchini F,Svoboda P,Salát J,Peace A,Gazumyan A,Cipolla M,Kapoor T,Guidetti F,Yao KH,Elsterová J,Teislerová D,Chrdle A,Hönig V,Oliveira T,West AP,Lee YE,Rice CM,MacDonald MR,Bjorkman PJ,Růžek D,Robbiani DF,Nussenzweig MC

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Mon May 03 00:00:00 UTC 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Functional assay

Thu Aug 26 00:00:00 UTC 2021

Preoperatively predicting the pathological types of acute appendicitis using machine learning based on peripheral blood biomarkers and clinical features: a retrospective study.

Annals of translational medicine

Kang CB,Li XW,Hou SY,Chi XQ,Shan HF,Zhang QJ,Li XB,Zhang J,Liu TJ

11-0037-42 was used in Flow cytometry/Cell sorting to conclude that peripheral blood biomarkers can predict the pathological type of SA from PA and GPA.

Sat May 01 00:00:00 UTC 2021

Allergen-specific IgE levels and the ability of IgE-allergen complexes to cross-link determine the extent of CD23-mediated T-cell activation.

The Journal of allergy and clinical immunology

Villazala-Merino S,Rodriguez-Dominguez A,Stanek V,Campion NJ,Gattinger P,Hofer G,Froeschl R,Fae I,Lupinek C,Vrtala S,Breiteneder H,Keller W,Perkmann T,Nakamura R,Pickl WF,Valenta R,Eckl-Dorna J,Niederberger V

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Sun Mar 01 00:00:00 UTC 2020

IL-10 Restores MHC Class I Expression and Interferes With Immunity in Papillary Thyroid Cancer With Hashimoto Thyroiditis.

Endocrinology

Lu ZW,Hu JQ,Liu WL,Wen D,Wei WJ,Wang YL,Wang Y,Liao T,Ji QH

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

11-0037 was used in Flow cytometry/Cell sorting to study the HDAC inhibitor CG-745 and its function to enable the synergistic effects of the immune checkpoint inhibitor combination therapy in various cancers by suppressing tumor microenvironment.

Mon Sep 28 00:00:00 UTC 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Mon Sep 28 00:00:00 UTC 2020

IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.

Journal of Cancer

Hu JQ,Lei BW,Wen D,Ma B,Zhang TT,Lu ZW,Wei WJ,Wang YL,Wang Y,Li DS,Ji QH,Liao T

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunohistochemistry

Mon Sep 28 00:00:00 UTC 2020

TIRC7 inhibits Th1 cells by upregulating the expression of CTLA‑4 and STAT3 in mice with acute graft‑versus‑host disease.

Oncology reports

Zhu F,Qiu T,Zhu S,Zhao K,Chen C,Qiao J,Pan B,Yan Z,Chen W,Liu Q,Wu Q,Cao J,Sang W,Zeng L,Sun H,Li Z,Xu K

11-0037 was used in Flow cytometry/Cell sorting to deepen the understanding of the pathogenesis of acute graft-versus-host disease (GVHD) and provide novel approaches for controlling acute GVHD.

Wed Jul 01 00:00:00 UTC 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Thu Feb 06 00:00:00 UTC 2020

Human Liver Memory CD8⁺ T Cells Use Autophagy for Tissue Residence.

Cell reports

Swadling L,Pallett LJ,Diniz MO,Baker JM,Amin OE,Stegmann KA,Burton AR,Schmidt NM,Jeffery-Smith A,Zakeri N,Suveizdyte K,Froghi F,Fusai G,Rosenberg WM,Davidson BR,Schurich A,Simon AK,Maini MK

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in T-Cell Activation

Tue Jan 21 00:00:00 UTC 2020

Small-molecule MMP2/MMP9 inhibitor SB-3CT modulates tumor immune surveillance by regulating PD-L1.

Genome medicine

Ye Y,Kuang X,Xie Z,Liang L,Zhang Z,Zhang Y,Ma F,Gao Q,Chang R,Lee HH,Zhao S,Su J,Li H,Peng J,Chen H,Yin M,Peng C,Yang N,Wang J,Liu J,Liu H,Han L,Chen X

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Mon Sep 28 00:00:00 UTC 2020

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Mon Sep 28 00:00:00 UTC 2020

Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4⁺ T Cell Immunity.

Cell

Binnewies M,Mujal AM,Pollack JL,Combes AJ,Hardison EA,Barry KC,Tsui J,Ruhland MK,Kersten K,Abushawish MA,Spasic M,Giurintano JP,Chan V,Daud AI,Ha P,Ye CJ,Roberts EW,Krummel MF

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Thu Apr 18 00:00:00 UTC 2019

Integrating Ligand-Receptor Interactions and In Vitro Evolution for Streamlined Discovery of Artificial Nucleic Acid Ligands.

Molecular therapy. Nucleic acids

Zumrut HE,Batool S,Argyropoulos KV,Williams N,Azad R,Mallikaratchy PR

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Fri Sep 06 00:00:00 UTC 2019

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Stem cells international

Li C,Huang J,Zhu H,Shi Q,Li D,Ju X

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Tue Oct 01 00:00:00 UTC 2019

Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.

Cells

Rossatti P,Ziegler L,Schregle R,Betzler VM,Ecker M,Rossy J

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Mon Nov 04 00:00:00 UTC 2019

Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells.

Scientific reports

Levring TB,Kongsbak-Wismann M,Rode AKO,Al-Jaberi FAH,Lopez DV,Met Ö,Woetmann A,Bonefeld CM,Ødum N,Geisler C

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Western Blot

Wed Nov 13 00:00:00 UTC 2019

TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions.

Cell reports

Leng T,Akther HD,Hackstein CP,Powell K,King T,Friedrich M,Christoforidou Z,McCuaig S,Neyazi M,Arancibia-Cárcamo CV,Hagel J,Powrie F,Peres RS,Millar V,Ebner D,Lamichhane R,Ussher J,Hinks TSC,Marchi E,Willberg C,Klenerman P

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunocytochemistry

Tue Sep 17 00:00:00 UTC 2019

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2019

Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy.

Cell reports

Celis-Gutierrez J,Blattmann P,Zhai Y,Jarmuzynski N,Ruminski K,Grégoire C,Ounoughene Y,Fiore F,Aebersold R,Roncagalli R,Gstaiger M,Malissen B

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Tue Jun 11 00:00:00 UTC 2019

Synthetic TRuC receptors engaging the complete T cell receptor for potent anti-tumor response.

Nature communications

Baeuerle PA,Ding J,Patel E,Thorausch N,Horton H,Gierut J,Scarfo I,Choudhary R,Kiner O,Krishnamurthy J,Le B,Morath A,Baldeviano GC,Quinn J,Tavares P,Wei Q,Weiler S,Maus MV,Getts D,Schamel WW,Hofmeister R

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Western Blot

Tue May 07 00:00:00 UTC 2019

Label-free optical hemogram of granulocytes enhanced by artificial neural networks.

Optics express

Gupta RK,Chen M,Malcolm GPA,Hempler N,Dholakia K,Powis SJ

11-0037-42 was used in Flow cytometry/Cell sorting to demonstrate a comparison between artificial neural networks and principal component analysis to classify the key granulocyte cell lineages of neutrophils and eosinophils.

Mon May 13 00:00:00 UTC 2019

Impact of FAK Expression on the Cytotoxic Effects of CIK Therapy in Triple-Negative Breast Cancer.

Cancers

Pan MR,Wu CC,Kan JY,Li QL,Chang SJ,Wu CC,Li CL,Ou-Yang F,Hou MF,Yip HK,Luo CW

11-0037-42 was used in Flow Cytometry to find that focal adhesion kinase (FAK), a non-receptor protein tyrosine kinase that regulates several cellular functions in different cells, has the potential to regulate tumor cells sensitized to CIK cells.

Mon Dec 30 00:00:00 UTC 2019

STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Moreira D,Adamus T,Zhao X,Su YL,Zhang Z,White SV,Swiderski P,Lu X,DePinho RA,Pal SK,Kortylewski M

11-0037 was used in Flow cytometry/Cell sorting to show a novel strategy combining STAT3 inhibition with Toll-like Receptor 9 (TLR9) stimulation to unleash immune response against prostate cancers regardless of the genetic background.

Sat Dec 01 00:00:00 UTC 2018

Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.

International journal of nanomedicine

Pan H,Liu J,Deng W,Xing J,Li Q,Wang Z

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Mon Jul 02 00:00:00 UTC 2018

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

11-0037 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 UTC 2019

Astrocytic tight junctions control inflammatory CNS lesion pathogenesis.

The Journal of clinical investigation

Horng S,Therattil A,Moyon S,Gordon A,Kim K,Argaw AT,Hara Y,Mariani JN,Sawai S,Flodby P,Crandall ED,Borok Z,Sofroniew MV,Chapouly C,John GR

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunofluorescence

Tue Aug 01 00:00:00 UTC 2017

KRAS mutation-induced upregulation of PD-L1 mediates immune escape in human lung adenocarcinoma.

Cancer immunology, immunotherapy : CII

Chen N,Fang W,Lin Z,Peng P,Wang J,Zhan J,Hong S,Huang J,Liu L,Sheng J,Zhou T,Chen Y,Zhang H,Zhang L

11-0037 was used in Flow cytometry/Cell sorting to demonstrate that Kirsten rat sarcoma viral oncogene homologue (KRAS) mutation could induce PD-L1 expression through p-ERK signalling in lung adenocarcinoma.

Fri Sep 01 00:00:00 UTC 2017

Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse.

Traffic (Copenhagen, Denmark)

Tello-Lafoz M,Martínez-Martínez G,Rodríguez-Rodríguez C,Albar JP,Huse M,Gharbi S,Merida I

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in T-Cell Activation

Tue Aug 01 00:00:00 UTC 2017

NKG2D Ligand-Targeted Bispecific T-Cell Engagers Lead to Robust Antitumor Activity against Diverse Human Tumors.

Molecular cancer therapeutics

Godbersen C,Coupet TA,Huehls AM,Zhang T,Battles MB,Fisher JL,Ernstoff MS,Sentman CL

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2017

Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.

Immunology and cell biology

Hernández Del Pino RE,Pellegrini JM,Rovetta AI,Peña D,Álvarez GI,Rolandelli A,Musella RM,Palmero DJ,Malbran A,Pasquinelli V,García VE

Fri Sep 01 00:00:00 UTC 2017

Cytochrome P450s in human immune cells regulate IL-22 and c-Kit via an AHR feedback loop.

Scientific reports

Effner R,Hiller J,Eyerich S,Traidl-Hoffmann C,Brockow K,Triggiani M,Behrendt H,Schmidt-Weber CB,Buters JT

11-0037 was used in Flow cytometry/Cell sorting to study the interdependence of Cytochrome P4501 enzymes and aryl hydrocarbon receptor in human primary immune cells.

Thu Mar 09 00:00:00 UTC 2017

Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination.

Proceedings of the National Academy of Sciences of the United States of America

Pageon SV,Tabarin T,Yamamoto Y,Ma Y,Nicovich PR,Bridgeman JS,Cohnen A,Benzing C,Gao Y,Crowther MD,Tungatt K,Dolton G,Sewell AK,Price DA,Acuto O,Parton RG,Gooding JJ,Rossy J,Rossjohn J,Gaus K

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunofluorescence

Tue Sep 13 00:00:00 UTC 2016

Obesity decreases B cell responses in young and elderly individuals.

Obesity (Silver Spring, Md.)

Frasca D,Ferracci F,Diaz A,Romero M,Lechner S,Blomberg BB

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in T-Cell Activation

Tue Mar 01 00:00:00 UTC 2016

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in T-Cell Activation

Wed Nov 20 00:00:00 UTC 2019

Formin-generated actomyosin arcs propel T cell receptor microcluster movement at the immune synapse.

The Journal of cell biology

Murugesan S,Hong J,Yi J,Li D,Beach JR,Shao L,Meinhardt J,Madison G,Wu X,Betzig E,Hammer JA

11-0037 was used in Flow cytometry/Cell sorting to reveal the derivation and organisation of actin arcs in the immunological synapse.

Mon Nov 07 00:00:00 UTC 2016

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2020

T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs.

Molecular biology of the cell

Guillou L,Babataheri A,Saitakis M,Bohineust A,Dogniaux S,Hivroz C,Barakat AI,Husson J

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunomicroscopy

Mon Nov 07 00:00:00 UTC 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2016

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.

Oncotarget

You Y,Tan JX,Dai HS,Chen HW,Xu XJ,Yang AG,Zhang YJ,Bai LH,Bie P

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Tue Aug 30 00:00:00 UTC 2016

Upregulation of PD-L1 by EML4-ALK fusion protein mediates the immune escape in ALK positive NSCLC: Implication for optional anti-PD-1/PD-L1 immune therapy for ALK-TKIs sensitive and resistant NSCLC patients.

Oncoimmunology

Hong S,Chen N,Fang W,Zhan J,Liu Q,Kang S,He X,Liu L,Zhou T,Huang J,Chen Y,Qin T,Zhang Y,Ma Y,Yang Y,Zhao Y,Huang Y,Zhang L

11-0037 was used in Flow cytometry/Cell sorting to explore how PD-L1 was regulated by ALK-TKIs fusion protein in ALK positive non-small-cell lung cancer.

Tue Mar 01 00:00:00 UTC 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2015

Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.

eLife

Kumari S,Depoil D,Martinelli R,Judokusumo E,Carmona G,Gertler FB,Kam LC,Carman CV,Burkhardt JK,Irvine DJ,Dustin ML

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunofluorescence

Wed Mar 11 00:00:00 UTC 2015

Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.

eLife

Kumari S,Depoil D,Martinelli R,Judokusumo E,Carmona G,Gertler FB,Kam LC,Carman CV,Burkhardt JK,Irvine DJ,Dustin ML

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunofluorescence

Wed Mar 11 00:00:00 UTC 2015

A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.

Blood cancer journal

Kim SY,Theunissen JW,Balibalos J,Liao-Chan S,Babcock MC,Wong T,Cairns B,Gonzalez D,van der Horst EH,Perez M,Levashova Z,Chinn L,D'Alessio JA,Flory M,Bermudez A,Jackson DY,Ha E,Monteon J,Bruhns MF,Chen G,Migone TS

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Fri May 29 00:00:00 UTC 2015

Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.

Molecular cell

Guo YE,Riley KJ,Iwasaki A,Steitz JA

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Western Blot

Thu Apr 10 00:00:00 UTC 2014

Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.

Proceedings of the National Academy of Sciences of the United States of America

Kumaresan PR,Manuri PR,Albert ND,Maiti S,Singh H,Mi T,Roszik J,Rabinovich B,Olivares S,Krishnamurthy J,Zhang L,Najjar AM,Huls MH,Lee DA,Champlin RE,Kontoyiannis DP,Cooper LJ

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Tue Jul 22 00:00:00 UTC 2014

N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.

The Journal of biological chemistry

Mellergaard M,Skovbakke SL,Schneider CL,Lauridsen F,Andresen L,Jensen H,Skov S

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in T-Cell Activation

Fri Jul 18 00:00:00 UTC 2014

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Flow Cytometry

Mon Sep 08 00:00:00 UTC 2014

Human and mouse CD137 have predominantly different binding CRDs to their respective ligands.

PloS one

Yi L,Zhao Y,Wang X,Dai M,Hellström KE,Hellström I,Zhang H

11-0037 was used in Flow cytometry/Cell sorting to better understand the action of agonistic and antagonistic anti-CD137 mAbs by mapping the binding region of the CD137 ligand to human and mouse CD137.

Mon Sep 15 00:00:00 UTC 2014

Autologous Graft versus Host Disease: An Emerging Complication in Patients with Multiple Myeloma.

Bone marrow research

Batra A,Cottler-Fox M,Harville T,Rhodes-Clark BS,Makhoul I,Nakagawa M

11-0037 was used in Flow cytometry/Cell sorting to compare expression of cell surface molecules on hematopoietic progenitor cell products between healthy individuals and sufferers of autologous graft versus host disease.

Fri May 30 00:00:00 UTC 2014

Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Bilwani FA,Knight KL

11-0037 was used in Flow cytometry/Cell sorting to investigate the factors contributing to an observed age-related decline in B lymphopoiesis.

Thu Nov 01 00:00:00 UTC 2012

ChIP-on-chip analysis identifies IL-22 as direct target gene of ectopically expressed FOXP3 transcription factor in human T cells.

BMC genomics

Jeron A,Hansen W,Ewert F,Buer J,Geffers R,Bruder D

11-0037 was used in Flow cytometry/Cell sorting to analyse human FOXP3 ectopically expressed in a Jurkat T-cell line under resting and mitogen-stimulated conditions to identify its direct target genes.

Mon Dec 17 00:00:00 UTC 2012

Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.

PloS one

Yu Y,Zitzner JR,Houlihan J,Herrera N,Xu L,Miller J,Mathew JM,Tambur AR,Luo X

11-0037 was used in Flow cytometry/Cell sorting to demonstrate the use of common γc cytokines in combination with donor APCs as a strategy for generation of donor-specific T cells for immunotherapy.

Mon Aug 27 00:00:00 UTC 2012

Mode of transmission affects the sensitivity of human immunodeficiency virus type 1 to restriction by rhesus TRIM5alpha.

Journal of virology

Richardson MW,Carroll RG,Stremlau M,Korokhov N,Humeau LM,Silvestri G,Sodroski J,Riley JL

11-0037 was used in Flow cytometry/Cell sorting to investigate the use of Rhesus TRIM5alpha for treatment of HIV.

Sat Nov 01 00:00:00 UTC 2008

Mode of transmission affects the sensitivity of human immunodeficiency virus type 1 to restriction by rhesus TRIM5alpha.

Journal of virology

Richardson MW,Carroll RG,Stremlau M,Korokhov N,Humeau LM,Silvestri G,Sodroski J,Riley JL

11-0037 was used in Flow cytometry/Cell sorting to investigate the use of Rhesus TRIM5alpha for treatment of HIV.

Sat Nov 01 00:00:00 UTC 2008

Production of multivalent protein binders using a self-trimerizing collagen-like peptide scaffold.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Fan CY,Huang CC,Chiu WC,Lai CC,Liou GG,Li HC,Chou MY

11-0037 was used in Flow cytometry/Cell sorting to design a class of multivalent protein binders that use a triplex-forming collagen-like peptide.

Sat Nov 01 00:00:00 UTC 2008

Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

PLoS medicine

Senechal B,Elain G,Jeziorski E,Grondin V,Patey-Mariaud de Serre N,Jaubert F,Beldjord K,Lellouch A,Glorion C,Zerah M,Mary P,Barkaoui M,Emile JF,Boccon-Gibod L,Josset P,Debré M,Fischer A,Donadieu J,Geissmann F

Published figure using CD3 monoclonal antibody (Product # 11-0037-42) in Immunohistochemistry

Wed Aug 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding

Process(es)

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CD3 Monoclonal Antibody (OKT3), FITC, eBioscience™

CD3 Antibody (11-0037-42) in Flow

Product Details

11-0037-42

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

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T-Cell Activation (TCA)

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