Invitrogen

CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), eBioscience™

CD3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

CD3 Antibody (14-0030-82) in ICC/IF

Immunofluorescence analysis of CD3 was performed using 70% confluent log phase EL4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), eBioscience™ (Product # 14-0030-82, 14-0030-81) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa ... View More

CD3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), eBioscience™ (Product # 14-0030-81), shows expression of CD3 in EL4 cells as compared to 8A3B.6 cells. {RE}

Product Details

14-0030-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-Dependent

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Rat

Published species

Non-human primate, Rat

Host/Isotype

Mouse / IgG3, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioG4.18 (G4.18)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467046

Product Specific Information

Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Preliminary analysis of immunoregulatory mechanism of hyperhomocysteinemia-induced brain injury in Wistar-Kyoto rats.

Experimental and therapeutic medicine

Zhang Y,Wang L,Li X,Geng J

Published figure using CD3 monoclonal antibody (Product # 14-0030-82) in Flow Cytometry

Sat May 01 00:00:00 UTC 2021

Genetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury.

Cell death & disease

Stark K,Goncharov T,Varfolomeev E,Xie L,Ngu H,Peng I,Anderson KR,Verschueren E,Choi M,Kirkpatrick DS,Easton A,Webster JD,McKenzie BS,Vucic D,Bingol B

Published figure using CD3 monoclonal antibody (Product # 14-0030-82) in Flow Cytometry

Wed Apr 07 00:00:00 UTC 2021

Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes.

Journal of inflammation research

Wang DD,Wu XY,Dong JY,Cheng XP,Gu SF,Olatunji OJ,Li Y,Zuo J

Published figure using CD3 monoclonal antibody (Product # 14-0030-82) in Flow Cytometry

Fri Apr 05 00:00:00 UTC 2024

Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose.

Frontiers in immunology

Steines L,Poth H,Schuster A,Amann K,Banas B,Bergler T

Published figure using CD3 monoclonal antibody (Product # 14-0030-82) in Flow Cytometry

Tue Sep 28 00:00:00 UTC 2021

Survival and Functionality of Human Induced Pluripotent Stem Cell-Derived Oligodendrocytes in a Nonhuman Primate Model for Multiple Sclerosis.

Stem cells translational medicine

Thiruvalluvan A,Czepiel M,Kap YA,Mantingh-Otter I,Vainchtein I,Kuipers J,Bijlard M,Baron W,Giepmans B,Brück W,'t Hart BA,Boddeke E,Copray S

14-0030 was used in Immunohistochemistry to study the use of human induced pluripotent stem cell-derived oligodendrocyte precursor cells in a nonhuman primate model of progressive multiple sclerosis.

Tue Nov 01 00:00:00 UTC 2016

Subconjunctivally applied naïve Tregs support corneal graft survival in baby rats.

Molecular vision

Hildebrand A,Jarsch C,Kern Y,Böhringer D,Reinhard T,Schwartzkopff J

14-0030 was used in Functional assays to investigate the relevance of naïve Tregs in juvenile corneal transplantation.

Tue Dec 08 00:00:00 UTC 2015

Molecular pathology of murine ureteritis causing obstructive uropathy with hydronephrosis.

PloS one

Ichii O,Otsuka S,Namiki Y,Hashimoto Y,Kon Y

Published figure using CD3 monoclonal antibody (Product # 14-0030-82) in Immunohistochemistry

Thu May 03 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CD247 antigen; CD3 antigen delta polypeptide; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma polypeptide; CD3 TCR complex; CD3d; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: Cd3d; Cd3g; Cd3z; T3d; TCRzeta

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UniProt ID: (Rat) P19377, (Rat) Q64159

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Entrez Gene ID: (Rat) 25300, (Rat) 25710, (Rat) 315609, (Rat) 300678

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Function(s)

molecular_function transmembrane signaling receptor activity identical protein binding transcription coactivator activity protein heterodimerization activity signal transducer activity SH3 domain binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), eBioscience™

CD3 Antibody (14-0030-82) in ICC/IF

Product Details

14-0030-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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