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Invitrogen
CD300f (IREM-1) Monoclonal Antibody (UP-D1), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
CD300f (IREM-1) Antibody (50-3008-42) in Flow
Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (blue histogram) or Anti-Human CD300f (IREM-1) eFluor® 660 (purple histogram). Cells in the monocyte gate were used for analysis.
Product Details
50-3008-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
UP-D1
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574195
Product Specific Information
Description: The monoclonal antibody recognizes human IREM-1 (Immune receptor expressed by myeloid cell 1). IREM-1 is also a member of of the CD300 family specifically referred to as CD300f. IREM-1 is a type I transmembrane protein with a single Ig domain, one predicted N-linked glycosylation site and a long cytoplasmic tail which contains a predicted ITIM domain categorizing it as an inhibitory receptor. Additionally the cytoplasmic domain has been shown to interact with SHP-1 and the p85alpha subunit of PI3K. Expression is restricted to myeloid cells. In peripheral blood IREM-1 is found on monocytes and granulocytes. In monocyte derived dendritic cells, expression of IREM-1 is downregulated.
Applications Reported: This UP-D1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This UP-D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CLM1 is an inhibitory receptor of the Ig superfamily expressed on myeloid cells. It mediates negative regulatory signals by recruiting SHP1 (PTPN6; MIM 176883) or SHIP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD300 antigen-like family member F; CD300 molecule-like family member f; CD300f; CLM-1; CMRF35-like molecule 1; IgSF13; Immune receptor expressed on myeloid cells 1; immunoglobin superfamily member 13; Immunoglobulin superfamily member 13; inhibitory receptor IREM1; IREM-1; NK inhibitory receptor
Gene Aliases: CD300F; CD300LF; CLM-1; CLM1; IGSF13; IREM-1; IREM1; LMIR3; NKIR; UNQ3105/PRO10111
UniProt ID: (Human) Q8TDQ1
Entrez Gene ID: (Human) 146722
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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