Invitrogen

CD304 (Neuropilin-1) Monoclonal Antibody (XML1Y1), eBioscience™

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

14-3042-82

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL
-

Immunocytochemistry (ICC/IF)

1 - 5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

XML1Y1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572873

Product Specific Information

Description: The monoclonal antibody XML1Y1 recognizes CD304 (Neuropilin-1), a type 1 transmembrane glycoprotein present on the surface of multiple cell types, including: regulatory T cells (Tregs), NKT cells, DC, certain types of stem cells, neurons, endothelial cells, and some neoplastic cells. Neuropilin-1, in complex with plexins, serves as a co-receptor for type 3 semaphorins in growing neurons. It is also involved in the process of angiogenesis being a part of a functional receptor for VEGF in endothelial cells. In mouse Neuropilin-1 is expressed on thymus-derived natural Tregs but not peripherally induced Treg cells. In addition, transient expression on recently activated non-regulatory T cells has been observed. Neuropilin-1 has been used as a marker of recent thymic emigrants in the mouse iNKT cell population. It has been shown that Neuropilin-1 forms a complex with TGF beta receptors, activating TGF beta latent form (LAP-TGF beta 1) and augmenting canonic Smad2/3 signaling.

This XML1Y1 antibody recognizes neuropilin-1 isoforms 1 and 2.

Applications Reported: This XML1Y1 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunocytochemistry.

Applications Tested: This XML1Y1 antibody has been tested by immunohistochemistry of acetone-fixed frozen tissue sections and can be used at less than or equal to 1 µg/mL. This XML1Y1 antibody has been tested by immunocytochemistry of methanol-fixed bEND3 cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A5 protein; CD304; Neuropilin; Neuropilin-1; Neuropilin-1 precursor (A5 protein); Neuropilin1; NRP 1; sNRP 1; sNRP1; soluble NRP 1; soluble NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor

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Gene Aliases: BDCA4; C530029I03; CD304; NP-1; NP1; NPN-1; Npn1; NRP; NRP1; VEGF165R

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UniProt ID: (Human) O14786, (Mouse) P97333

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Entrez Gene ID: (Human) 8829, (Mouse) 18186

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Function(s)
vascular endothelial growth factor-activated receptor activity protein binding heparin binding coreceptor activity semaphorin receptor activity growth factor binding cytokine binding vascular endothelial growth factor binding metal ion binding
Process(es)
angiogenesis patterning of blood vessels neuron migration positive regulation of endothelial cell proliferation sprouting angiogenesis cell migration involved in sprouting angiogenesis signal transduction cell-cell signaling axon guidance axonal fasciculation response to wounding organ morphogenesis positive regulation of endothelial cell migration positive regulation of smooth muscle cell migration dendrite development facial nerve structural organization trigeminal nerve structural organization vestibulocochlear nerve structural organization nerve development branchiomotor neuron axon guidance gonadotrophin-releasing hormone neuronal migration to the hypothalamus retinal ganglion cell axon guidance cellular response to hepatocyte growth factor stimulus endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus ventral trunk neural crest cell migration VEGF-activated neuropilin signaling pathway otic placode formation negative regulation of neuron apoptotic process platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway hepatocyte growth factor receptor signaling pathway positive regulation of axon extension involved in axon guidance negative regulation of axon extension involved in axon guidance artery morphogenesis axon extension involved in axon guidance positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of cytokine activity axonogenesis involved in innervation regulation of vesicle-mediated transport dichotomous subdivision of terminal units involved in salivary gland branching angiogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis retina vasculature morphogenesis in camera-type eye renal artery morphogenesis sympathetic ganglion development trigeminal ganglion development positive regulation of ERK1 and ERK2 cascade semaphorin-plexin signaling pathway commissural neuron axon guidance regulation of retinal ganglion cell axon guidance endothelial tip cell fate specification sensory neuron axon guidance sympathetic neuron projection extension sympathetic neuron projection guidance neural crest cell migration involved in autonomic nervous system development toxin transport semaphorin-plexin signaling pathway involved in neuron projection guidance semaphorin-plexin signaling pathway involved in axon guidance positive regulation of retinal ganglion cell axon guidance VEGF-activated neuropilin signaling pathway involved in axon guidance protein localization to early endosome facioacoustic ganglion development dorsal root ganglion morphogenesis negative regulation of extrinsic apoptotic signaling pathway multicellular organism development nervous system development heart development motor neuron axon guidance cell migration peptidyl-tyrosine phosphorylation trigeminal nerve morphogenesis cell differentiation negative regulation of axon extension vascular endothelial growth factor signaling pathway sympathetic nervous system development neuron development regulation of axon extension involved in axon guidance cell migration involved in coronary vasculogenesis ganglion morphogenesis
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