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Invitrogen
CD305 (LAIR1) Monoclonal Antibody (113), eBioscience™
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-Flow-20170216130641.jpg?time=20250104 "CD305 (LAIR1) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 1
CD305 (LAIR1) Antibody (14-3051-82) in Flow
Staining of C57BL/6 splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (purple line) or 0.125 µg of Anti-Mouse CD305 (LAIR-1) Purified (blue line) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83). Total viable F4/80+ cells were used for analysis.
-Flow-20170216130641.jpg?time=20250104)
-Flow-20170216130641.jpg?time=20250104?time=20250104 "CD305 (LAIR1) Antibody (14-3051-82) in Flow")
Product Details
14-3051-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
113
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1210499
Product Specific Information
Description: CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.
Applications Reported: This 113 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).
Applications Tested: This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD305, also known as LAIR1, is a type I transmembrane glycoprotein with an extracellular Ig-like domain and two intracellular ITIMs. It is expressed on a wide range of immune cells, including T, NK, and B cells, monocytes, dendritic cells, eosinophils, basophils, and mast cells. In humans, CD305 is also present on CD34+ hematopoietic progenitor cells and thymocytes. Several isoforms exist due to alternative splicing, with expression varying between cell types. CD305 functions as an inhibitory receptor, playing a key role in regulating immune responses. It blocks cytokine-mediated signaling, cell proliferation, and apoptosis. In NK, B, and T cells, CD305 modulates cytolytic functions and cytokine production, down-regulating IL2 and IFNG in CD4+ T cells while inducing transforming growth factor beta. In B cells, it reduces IgG and IgE production and decreases IL8, IL10, and TNF secretion. Activation of CD305 through tyrosine phosphorylation recruits phosphatases PTPN6 and PTPN11, although it may also function independently of SH2-containing phosphatases. It inhibits differentiation of peripheral blood precursors into dendritic cells and has been shown to induce apoptosis in myeloid leukemia cell lines. Collagens are identified as ligands for CD305, underscoring its role in immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD305; LAIR-1; Leukocyte-associated immunoglobulin-like receptor 1
Gene Aliases: 5133400O11Rik; BB115266; D7Bwg0421e; Lair-1; Lair1
UniProt ID: (Mouse) Q8BG84
Entrez Gene ID: (Mouse) 52855
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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