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Invitrogen
CD307e (FcRL5) Monoclonal Antibody (509F6), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
CD307e (FcRL5) Antibody (50-3078-42) in Flow
Staining of normal human peripheral blood cells (left) and human tonsil cells (right) with Anti-Human CD19 eFluor® 450 (Product # 48-0198-42) and Anti-Human CD307e (FcRL5) eFluor® 660 (Product # 50-3078-42). Cells in the lymphocyte gate were used for analysis. Data courtesy of the laboratory of Shane Crotty.
Product Details
50-3078-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
509F6
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574199
Product Specific Information
Description: The 509F6 monoclonal antibody reacts with human CD307e which is also known as Fc receptor-like 5, FcRL5, FcRH5, or IRTA2. CD307e is a surface protein expressed selectively on naive mature B cells and on bone marrow plasma cells, and binds to all IgG isotypes. CD307e functions as an inhibitory coreceptor on mature B cells in humans via the recruitment of the phosphatase SHP-1 to its two immunoreceptor tyrosine-based inhibitory motifs (ITIM). CD307e can also exist in soluble form and high levels of soluble CD307e have been detected in the blood of patients with multiple myeloma, chronic lymphocytic leukemia, and mantle cell lymphoma. Because surface-bound CD307e is also highly-expressed on malignant B cells from multiple myeloma, chronic lymphocytic leukemia, and mantle cell lymphoma patients, CD307e may be a useful therapeutic target for the treatment of these cancers.
Applications Reported: This 509F6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 509F6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Fc receptor homologs (FcRH5), also known as IRTA2 proteins, are transmembrane proteins with sequence homology to classical Fc receptor families. FcRH5 function as activating or inhibitory receptors on discrete B cell populations and are involved in B-cell development and differentiation in peripheral lymphoid organs. FcRH5 expression is deregulated in multiple myeloma and Burkitt lymphoma cell lines with 1q21 abnormalities. FcRH5 may play a critical role for the pathogenesis of B-cell lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: BXMAS1; CD307e; Fc receptor homolog 5; Fc receptor-like 5; Fc receptor-like protein 5; FcR-like protein 5; FcRH5; Immune receptor translocation-associated protein 2; immunoglobulin superfamily receptor translocation associated 2 (IRTA2)
Gene Aliases: BXMAS1; CD307; CD307e; FCRH5; FCRL5; IRTA2; PRO820; UNQ503/PRO820
UniProt ID: (Human) Q96RD9
Entrez Gene ID: (Human) 83416
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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