Invitrogen

CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience™

CD309 (FLK1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 50

CD309 (FLK1) Antibody (14-5821-82) in Flow

Surface staining of bEnd-3 cell line with Anti-Mouse CD309 (FLK1) PE.Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

CD309 (FLK1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD309 (FLK1) Antibody in Immunocytochemistry (ICC/IF)

CD309 (FLK1) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD309 (FLK1) Antibody in Immunohistochemistry (IHC)

CD309 (FLK1) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD309 (FLK1) Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

CD309 (FLK1) Antibody in Flow Cytometry, Peptide array (Flow, Array)

Product Image Gallery

Product Details

14-5821-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

Assay-Dependent

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 20 publications 20 publications

Peptide Array (Array)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Dog, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Avas12a1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467623

Product Specific Information

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported: The Avas12a1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: This Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function.

Nature communications

Wang Y,Elsherbiny A,Kessler L,Cordero J,Shi H,Serke H,Lityagina O,Trogisch FA,Mohammadi MM,El-Battrawy I,Backs J,Wieland T,Heineke J,Dobreva G

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Fri Nov 04 00:00:00 UTC 2022

Isolation of a unique hepatic stellate cell population expressing integrin α8 from embryonic mouse livers.

Developmental dynamics : an official publication of the American Association of Anatomists

Ogawa T,Li Y,Lua I,Hartner A,Asahina K

14-5821-82 was used in Immunohistochemistry to identify specific markers for embryonic hepatic stellate cells.

Fri Jun 01 00:00:00 UTC 2018

SCL/TAL1 cooperates with Polycomb RYBP-PRC1 to suppress alternative lineages in blood-fated cells.

Nature communications

Chagraoui H,Kristiansen MS,Ruiz JP,Serra-Barros A,Richter J,Hall-Ponselé E,Gray N,Waithe D,Clark K,Hublitz P,Repapi E,Otto G,Sopp P,Taylor S,Thongjuea S,Vyas P,Porcher C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Dec 18 00:00:00 UTC 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Mar 20 00:00:00 UTC 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Mar 20 00:00:00 UTC 2018

Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis.

JCI insight

Murray LA,Habiel DM,Hohmann M,Camelo A,Shang H,Zhou Y,Coelho AL,Peng X,Gulati M,Crestani B,Sleeman MA,Mustelin T,Moore MW,Ryu C,Osafo-Addo AD,Elias JA,Lee CG,Hu B,Herazo-Maya JD,Knight DA,Hogaboam CM,Herzog EL

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Aug 17 00:00:00 UTC 2017

Sustained inflammation after pericyte depletion induces irreversible blood-retina barrier breakdown.

JCI insight

Ogura S,Kurata K,Hattori Y,Takase H,Ishiguro-Oonuma T,Hwang Y,Ahn S,Park I,Ikeda W,Kusuhara S,Fukushima Y,Nara H,Sakai H,Fujiwara T,Matsushita J,Ema M,Hirashima M,Minami T,Shibuya M,Takakura N,Kim P,Miyata T,Ogura Y,Uemura A

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Thu Feb 09 00:00:00 UTC 2017

Endothelial cell-anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice.

Molecular carcinogenesis

Wang J,Xiao J,Wen D,Wu X,Mao Z,Zhang J,Ma D

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry (Paraffin)

Sun May 01 00:00:00 UTC 2016

The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

Cell stem cell

Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Sep 03 00:00:00 UTC 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Tue Jul 14 00:00:00 UTC 2015

Mesenchymal-endothelial transition contributes to cardiac neovascularization.

Nature

Ubil E,Duan J,Pillai IC,Rosa-Garrido M,Wu Y,Bargiacchi F,Lu Y,Stanbouly S,Huang J,Rojas M,Vondriska TM,Stefani E,Deb A

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Oct 30 00:00:00 UTC 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

14-5821 was used in Immunohistochemistry to explore a molecular cascade that is potentially an early step in the programming of ESC differentiation.

Sat Nov 01 00:00:00 UTC 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

14-5821 was used in Immunohistochemistry to explore a molecular cascade that is potentially an early step in the programming of ESC differentiation.

Sat Nov 01 00:00:00 UTC 2014

SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1).

Development (Cambridge, England)

Yamamizu K,Schlessinger D,Ko MS

14-5821 was used in Immunohistochemistry to explore a molecular cascade that is potentially an early step in the programming of ESC differentiation.

Sat Nov 01 00:00:00 UTC 2014

Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm.

Stem cells (Dayton, Ohio)

Zhang H,Nieves JL,Fraser ST,Isern J,Douvaras P,Papatsenko D,D'Souza SL,Lemischka IR,Dyer MA,Baron MH

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.

Developmental biology

Shi X,Richard J,Zirbes KM,Gong W,Lin G,Kyba M,Thomson JA,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu May 15 00:00:00 UTC 2014

Nkx2-5 mediates differential cardiac differentiation through interaction with Hoxa10.

Stem cells and development

Behrens AN,Iacovino M,Lohr JL,Ren Y,Zierold C,Harvey RP,Kyba M,Garry DJ,Martin CM

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Thu Aug 01 00:00:00 UTC 2013

Etv2 rescues Flk1 mutant embryoid bodies.

Genesis (New York, N.Y. : 2000)

Rasmussen TL,Martin CM,Walter CA,Shi X,Perlingeiro R,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Mon Jul 01 00:00:00 UTC 2013

Eomesodermin induces Mesp1 expression and cardiac differentiation from embryonic stem cells in the absence of Activin.

EMBO reports

van den Ameele J,Tiberi L,Bondue A,Paulissen C,Herpoel A,Iacovino M,Kyba M,Blanpain C,Vanderhaeghen P

14-5821 was used in Flow cytometry/Cell sorting to investigate the range of cell fates controlled by Eomes and its mechanisms of action.

Sun Apr 01 00:00:00 UTC 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunofluorescence

Wed Aug 01 00:00:00 UTC 2012

Etv2 is expressed in the yolk sac hematopoietic and endothelial progenitors and regulates Lmo2 gene expression.

Stem cells (Dayton, Ohio)

Koyano-Nakagawa N,Kweon J,Iacovino M,Shi X,Rasmussen TL,Borges L,Zirbes KM,Li T,Perlingeiro RC,Kyba M,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunofluorescence

Wed Aug 01 00:00:00 UTC 2012

Tumor-expressed inducible nitric oxide synthase controls induction of functional myeloid-derived suppressor cells through modulation of vascular endothelial growth factor release.

Journal of immunology (Baltimore, Md. : 1950)

Jayaraman P,Parikh F,Lopez-Rivera E,Hailemichael Y,Clark A,Ma G,Cannan D,Ramacher M,Kato M,Overwijk WW,Chen SH,Umansky VY,Sikora AG

14-5821 was used in Flow cytometry/Cell sorting to suggest a critical role for tumor-expressed inducible NO synthase in recruitment and induction of functional myeloid-derived suppressor cells.

Fri Jun 01 00:00:00 UTC 2012

VEGF/Flk1 signaling cascade transactivates Etv2 gene expression.

PloS one

Rasmussen TL,Shi X,Wallis A,Kweon J,Zirbes KM,Koyano-Nakagawa N,Garry DJ

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Mon May 13 00:00:00 UTC 2013

Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair.

PloS one

Sountoulidis A,Stavropoulos A,Giaglis S,Apostolou E,Monteiro R,Chuva de Sousa Lopes SM,Chen H,Stripp BR,Mummery C,Andreakos E,Sideras P

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunofluorescence

Wed May 15 00:00:00 UTC 2013

Activation of the canonical bone morphogenetic protein (BMP) pathway during lung morphogenesis and adult lung tissue repair.

PloS one

Sountoulidis A,Stavropoulos A,Giaglis S,Apostolou E,Monteiro R,Chuva de Sousa Lopes SM,Chen H,Stripp BR,Mummery C,Andreakos E,Sideras P

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunofluorescence

Wed May 15 00:00:00 UTC 2013

Whole-mount confocal microscopy for vascular branching morphogenesis.

Methods in molecular biology (Clifton, N.J.)

Mukouyama YS,James J,Nam J,Uchida Y

14-5821 was used in Immunohistochemistry-wholemount to describe a protocol to stain intact blood vessels with vascular-specific antibodies and fluorescent secondary antibodies.

Thu Apr 26 00:00:00 UTC 2012

Recapitulation of the embryonic cardiovascular progenitor cell niche.

Biomaterials

Schenke-Layland K,Nsair A,Van Handel B,Angelis E,Gluck JM,Votteler M,Goldhaber JI,Mikkola HK,Kahn M,Maclellan WR

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Fri Apr 01 00:00:00 UTC 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Fri Jul 01 00:00:00 UTC 2011

A triple-targeted ultrasound contrast agent provides improved localization to tumor vasculature.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine

Warram JM,Sorace AG,Saini R,Umphrey HR,Zinn KR,Hoyt K

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunohistochemistry

Fri Jul 01 00:00:00 UTC 2011

The effects of VEGF-R1 and VEGF-R2 ligands on angiogenic responses and left ventricular function in mice.

Cardiovascular research

Huusko J,Merentie M,Dijkstra MH,Ryhänen MM,Karvinen H,Rissanen TT,Vanwildemeersch M,Hedman M,Lipponen J,Heinonen SE,Eriksson U,Shibuya M,Ylä-Herttuala S

14-5821 was used in Immunohistochemistry on paraffin embedded tissues to analyse the angiogenic properties of ligands for different VEGF receptors in mouse myocardium.

Thu Apr 01 00:00:00 UTC 2010

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Stem cells (Dayton, Ohio)

Ma M,Ding S,Lundqvist A,San H,Fang F,Konoplyannikov M,Berry C,Beltran LE,Chen G,Kovacic JC,Boehm M

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Wed Sep 01 00:00:00 UTC 2010

Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival.

Stem cells (Dayton, Ohio)

Ma M,Ding S,Lundqvist A,San H,Fang F,Konoplyannikov M,Berry C,Beltran LE,Chen G,Kovacic JC,Boehm M

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Flow Cytometry

Wed Sep 01 00:00:00 UTC 2010

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

14-5821 was used in Immunohistochemistry to investigate the mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells in mouse liver development.

Sun Mar 01 00:00:00 UTC 2009

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

14-5821 was used in Immunohistochemistry to investigate the mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells in mouse liver development.

Sun Mar 01 00:00:00 UTC 2009

Endogenous matrix metalloproteinase (MMP)-3 and MMP-9 promote the differentiation and migration of adult neural progenitor cells in response to chemokines.

Stem cells (Dayton, Ohio)

Barkho BZ,Munoz AE,Li X,Li L,Cunningham LA,Zhao X

Published figure using CD309 (FLK1) monoclonal antibody (Product # 14-5821-82) in Immunocytochemistry

Mon Dec 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

View more View less

Gene Aliases: 6130401C07; Flk-1; Flk1; Kdr; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2

View more View less

UniProt ID: (Mouse) P35918

View more View less

Entrez Gene ID: (Mouse) 16542

View more View less

Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding vascular endothelial growth factor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience™

CD309 (FLK1) Antibody (14-5821-82) in Flow

Product Details

14-5821-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Peptide Array (Array)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!