Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD31 (PECAM-1) Antibodies

          Invitrogen

          CD31 Monoclonal Antibody (2H8)

          36 Published Figures
          61 References
          View all (297) CD31 (PECAM-1) antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD31 Monoclonal Antibody (2H8)

          • Antibody Testing Data (2)
          • Published Figures (36)
          CD31 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD31 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 38

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          CD31 Antibody (MA3105) in Flow

          Flow cytometry analysis of CD31 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA3105) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          CD31 Antibody in Flow Cytometry (Flow)
          CD31 Antibody in Flow Cytometry (Flow)
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD31 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)

          Product Details

          MA3105

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 40 publications 40 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 3 publications 3 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          -
          View 9 publications 9 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 3 publications 3 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          Assay-dependent
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Armenian hamster / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2H8

          Immunogen

          Mouse PECAM-1 (CD31)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_223592

          Product Specific Information

          MA3105 targets CD31 in FACS, and Neu applications and shows reactivity with mouse and Human samples.



          The MA3105 immunogen is mouse PECAM-1 (CD31).



          MA3105 detects CD31 which has a predicted molecular weight of approximately 79 kDa.



          This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

          Target Information

          CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

          View more View less

          Gene Aliases: C85791; CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; Pecam; PECAM-1; PECAM1

          View more View less

          UniProt ID: (Human) P16284, (Mouse) Q08481

          View more View less

          Entrez Gene ID: (Human) 5175, (Mouse) 18613

          View more View less

          Function(s)
          protein binding
          Process(es)
          platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67c77f6dc7-n6nf2:80/100.66.135.186:80.
          git-commit: 545e792480003146e539d624bd2a21b51d198228
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0