Invitrogen

CD31 Monoclonal Antibody (390), Pacific Blue™

5 Published Figures

10 References

View all (297) CD31 (PECAM-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CD31 Antibody (RM5228) in Flow

C57BL/6 mouse spleen cells stained with Pacific Blue™-conjugated anti-CD31 (Product # RM5228) (black line). The gray line represents spleen cells incubated with isotype control.

CD31 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

RM5228

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

390

Immunogen

Mouse 32D leukocyte cell line.

View immunogen

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10373114

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy.

Frontiers in cell and developmental biology

Parker E,Khayrullin A,Kent A,Mendhe B,Youssef El Baradie KB,Yu K,Pihkala J,Liu Y,McGee-Lawrence M,Johnson M,Chen J,Hamrick M

RM5228 was used in Flow Cytometry to investigate the role of FAPs in disuse atrophy in vivo utilizing a 2-week single hindlimb immobilization model.

Fri Jan 21 00:00:00 UTC 2022

Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice.

PLoS genetics

Madaro L,Torcinaro A,De Bardi M,Contino FF,Pelizzola M,Diaferia GR,Imeneo G,Bouchè M,Puri PL,De Santa F

Published figure using CD31 monoclonal antibody (Product # RM5228) in Flow Cytometry

Tue Oct 01 00:00:00 UTC 2019

PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration.

International journal of molecular sciences

Dammone G,Karaz S,Lukjanenko L,Winkler C,Sizzano F,Jacot G,Migliavacca E,Palini A,Desvergne B,Gilardi F,Feige JN

RM5228 was used in Flow cytometry/Cell sorting to study muscle regeneration and tissue-resident muscle stem cell function in whole body Pparg null mice generated by epiblast-specific Cre/lox deletion (PpargΔ/Δ).

Fri Jul 13 00:00:00 UTC 2018

Dynamics of cellular states of fibro-adipogenic progenitors during myogenesis and muscular dystrophy.

Nature communications

Malecova B,Gatto S,Etxaniz U,Passafaro M,Cortez A,Nicoletti C,Giordani L,Torcinaro A,De Bardi M,Bicciato S,De Santa F,Madaro L,Puri PL

RM5228 was used in Flow cytometry/Cell sorting to examine the dynamics of cellular states of fibro-adipogenic progenitors during myogenesis and muscular dystrophy.

Mon Sep 10 00:00:00 UTC 2018

Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells.

Methods in molecular biology (Clifton, N.J.)

Gatto S,Puri PL,Malecova B

RM5228 was used in flow cytometry to develop a procedure for isolating the skeletal muscle interstitial cells, termed Fibro-Adipogenic Progenitors, and their gene expression profiling at the single cell level

Mon Feb 26 00:00:00 UTC 2018

Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice.

Nature medicine

Lukjanenko L,Jung MJ,Hegde N,Perruisseau-Carrier C,Migliavacca E,Rozo M,Karaz S,Jacot G,Schmidt M,Li L,Metairon S,Raymond F,Lee U,Sizzano F,Wilson DH,Dumont NA,Palini A,Fässler R,Steiner P,Descombes P,Rudnicki MA,Fan CM,von Maltzahn J,Feige JN,Bentzinger CF

RM5228 was used in flow cytometry to show that the loss of stem cell adhesion to fibronectin is a mechanism of aging

Mon Aug 01 00:00:00 UTC 2016

TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells.

eLife

Malecova B,Dall'Agnese A,Madaro L,Gatto S,Coutinho Toto P,Albini S,Ryan T,Tora L,Puri PL

RM5228 was used in flow cytometry to analyze TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells

Thu Feb 25 00:00:00 UTC 2016

TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells.

eLife

Malecova B,Dall'Agnese A,Madaro L,Gatto S,Coutinho Toto P,Albini S,Ryan T,Tora L,Puri PL

RM5228 was used in flow cytometry to analyze TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells

Thu Feb 25 00:00:00 UTC 2016

Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization.

eLife

Incardona F,Doroudchi MM,Ismail N,Carreno A,Griner E,Anna Lim M

RM5228 was used in Flow Cytometry to describe the proposed replication plan of key experiments from ’ Interactions between cancer stem cells and their niche govern metastatic colonization’ by Malanchi and colleagues, published in Nature in 2012 (Malanchi et al., 2012).

Thu Jun 18 00:00:00 UTC 2015

Involvement of endothelial PECAM-1/CD31 in angiogenesis.

The American journal of pathology

DeLisser HM,Christofidou-Solomidou M,Strieter RM,Burdick MD,Robinson CS,Wexler RS,Kerr JS,Garlanda C,Merwin JR,Madri JA,Albelda SM

RM5228 was used in blocking or activating experiment and immunohistochemistry - frozen section to examine the effect of PECAM-1/ CD31 inhibition in rat and murine models of angiogenesis

Mon Sep 01 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

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Gene Aliases: C85791; Cd31; Pecam; PECAM-1; Pecam1

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UniProt ID: (Mouse) Q08481

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Entrez Gene ID: (Mouse) 18613

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD31 Monoclonal Antibody (390), Pacific Blue™

CD31 Antibody (RM5228) in Flow

Product Details

RM5228

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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