Proteintech

CD31 Monoclonal Antibody (3F8E2)

CD31 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD31 Antibody (66065-2-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

Product Details

66065-2-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10,000

Immunocytochemistry (ICC/IF)

View 17 publications 17 publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3F8E2

Immunogen

CD31 Fusion Protein Ag19730

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

565 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Biochimica et biophysica acta. Molecular basis of disease

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Wed Feb 01 00:00:00 UTC 2023

Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair.

Materials today. Bio

Wu F,Wang P,Wei X,Yang Y,Al Mamun A,Zhang X,Zhu Y,Mo T,Zhang H,Jiang C,Hu J,Xiao J

Wed Feb 01 00:00:00 UTC 2023

Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair.

Materials today. Bio

Wu F,Wang P,Wei X,Yang Y,Al Mamun A,Zhang X,Zhu Y,Mo T,Zhang H,Jiang C,Hu J,Xiao J

Wed Feb 01 00:00:00 UTC 2023

Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair.

Materials today. Bio

Wu F,Wang P,Wei X,Yang Y,Al Mamun A,Zhang X,Zhu Y,Mo T,Zhang H,Jiang C,Hu J,Xiao J

Wed Feb 01 00:00:00 UTC 2023

Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair.

Materials today. Bio

Wu F,Wang P,Wei X,Yang Y,Al Mamun A,Zhang X,Zhu Y,Mo T,Zhang H,Jiang C,Hu J,Xiao J

Wed Feb 01 00:00:00 UTC 2023

Attenuated Expression of SLCO2A1 Caused by DNA Methylation in Pediatric Inflammatory Bowel Disease.

Inflammatory bowel diseases

Ito N,Kudo T,Eguchi H,Jimbo K,Furuhata A,Okuno T,Takeuchi I,Arai K,Ishige T,Okazaki Y,Shimizu T

Tue Dec 05 00:00:00 UTC 2023

Circular RNA hsa_circ_0005239 contributes to hepatocellular carcinoma cell migration, invasion, and angiogenesis by targeting the miR-34a-5p/PD-L1 axis.

Cell biology international

He T,Huang J,Liang H,Zhong B,Xu G,Zhu X

Fri Sep 01 00:00:00 UTC 2023

Ergothioneine improves myocardial remodeling and heart function after acute myocardial infarction via S-glutathionylation through the NF-ĸB dependent Wnt5a-sFlt-1 pathway.

European journal of pharmacology

Duan R,Pan H,Li D,Liao S,Han B

Wed Jul 05 00:00:00 UTC 2023

Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells.

British journal of pharmacology

Jiang M,Zhang YX,Bu WJ,Li P,Chen JH,Cao M,Dong YC,Sun ZJ,Dong DL

Sat Jul 01 00:00:00 UTC 2023

Aconitum carmichaelii Debx. Attenuates Heart Failure through Inhibiting Inflammation and Abnormal Vascular Remodeling.

International journal of molecular sciences

Xing Z,Chen J,Yu T,Li X,Dong W,Peng C,Li D

Sun Mar 19 00:00:00 UTC 2023

A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.

Cell reports methods

Zhu J,Liu X,Xu J,Deng Y,Wang P,Liu Z,Yang Q,Li D,Yu T,Zhu D

Mon Feb 27 00:00:00 UTC 2023

E-Cigarette Aerosol Condensate Leads to Impaired Coronary Endothelial Cell Health and Restricted Angiogenesis.

International journal of molecular sciences

Chhor M,Tulpar E,Nguyen T,Cranfield CG,Gorrie CA,Chan YL,Chen H,Oliver BG,McClements L,McGrath KC

Tue Mar 28 00:00:00 UTC 2023

The anti-angiogenesis mechanism of Geniposide on rheumatoid arthritis is related to the regulation of PTEN.

Inflammopharmacology

Bu Y,Wu H,Deng R,Wang Y

Wed Jun 01 00:00:00 UTC 2022

Improvement of tube formation model of cell: Application for acute hypoxia in in vitro study of angiogenesis.

Microvascular research

Li F,Gou X,Xu D,Han D,Hou K,Fang W,Li Y

Tue Mar 01 00:00:00 UTC 2022

Improvement of tube formation model of cell: Application for acute hypoxia in in vitro study of angiogenesis.

Microvascular research

Li F,Gou X,Xu D,Han D,Hou K,Fang W,Li Y

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Blood plasma derived extracellular vesicles (BEVs): particle purification liquid chromatography (PPLC) and proteomic analysis reveals BEVs as a potential minimally invasive tool for predicting response to breast cancer treatment.

Breast cancer research and treatment

Alvarez FA,Kaddour H,Lyu Y,Preece C,Cohen J,Baer L,Stopeck AT,Thompson P,Okeoma CM

Tue Nov 01 00:00:00 UTC 2022

Paeoniflorin attenuates monocrotaline-induced pulmonary arterial hypertension in rats by suppressing TAK1-MAPK/NF-κB pathways.

International journal of medical sciences

Yu M,Wu X,Wang J,He M,Han H,Hu S,Xu J,Yang M,Tan Q,Wang Y,Wang H,Xie W,Kong H

Thu May 19 00:00:00 UTC 2022

Indole-3-propionic Acid Attenuates HI-Related Blood-Brain Barrier Injury in Neonatal Rats by Modulating the PXR Signaling Pathway.

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Qihuang Zhuyu Formula Attenuates Atherosclerosis via Targeting PPARγ to Regulate Cholesterol Efflux and Endothelial Cell Inflammation.

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Wang M,Xiang Q,Sun W,Zhang H,Shi R,Guo J,Tong H,Fan M,Ding Y,Shi H,Yu P,Shen L,Wang Q,Chen X

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Exosomal myeloperoxidase as a biomarker of deep venous thrombosis.

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Xing Z,Yang C,He J,Feng Y,Li X,Peng C,Li D

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Cardioprotective Effects of Aconite in Isoproterenol-Induced Myocardial Infarction in Rats.

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Liu J,Xu J,Huang J,Gu C,Liu Q,Zhang W,Gao F,Tian Y,Miao X,Zhu Z,Jia B,Tian Y,Wu L,Zhao H,Feng X,Liu S

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TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway.

Laboratory investigation; a journal of technical methods and pathology

Liu J,Xu J,Huang J,Gu C,Liu Q,Zhang W,Gao F,Tian Y,Miao X,Zhu Z,Jia B,Tian Y,Wu L,Zhao H,Feng X,Liu S

Sun Aug 01 00:00:00 UTC 2021

TRIM27 contributes to glomerular endothelial cell injury in lupus nephritis by mediating the FoxO1 signaling pathway.

Laboratory investigation; a journal of technical methods and pathology

Liu J,Xu J,Huang J,Gu C,Liu Q,Zhang W,Gao F,Tian Y,Miao X,Zhu Z,Jia B,Tian Y,Wu L,Zhao H,Feng X,Liu S

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International journal of biological sciences

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KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy.

International journal of biological sciences

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Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

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Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

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UniProt ID: (Human) P16284

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Entrez Gene ID: (Human) 5175

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Function(s)

protein binding

Process(es)

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD31 Monoclonal Antibody (3F8E2)

CD31 Antibody (66065-2-IG) in IHC (P)

Product Details

66065-2-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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