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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD31 (PECAM-1) Antibodies

          Invitrogen

          CD31 Monoclonal Antibody (HEC7)

          Advanced Verification
          19 Published Figures
          37 References
          View all (297) CD31 (PECAM-1) antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite CD31 Monoclonal Antibody (HEC7)

          • Antibody Testing Data (6)
          • Published Figures (19)
          • Advanced Verification (2)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 27

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          CD31 Antibody (MA3100) in ICC/IF

          Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Monoclonal Antibody (HEC7) (Product # MA3100) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antib... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Western Blot (WB)
          CD31 Antibody in Flow Cytometry (Flow)
          CD31 Antibody in Flow Cytometry (Flow)
          CD31 Antibody in Flow Cytometry (Flow)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          CD31 Antibody in Immunocytochemistry (ICC/IF)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Immunohistochemistry (IHC)
          CD31 Antibody in Neutralization (Neu)
          CD31 Antibody
          CD31 Antibody

          Product Details

          MA3100

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 12 publications 12 publications

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 10 publications 10 publications

          Flow Cytometry (Flow)

          3-5 µg/1x10^6 cells
          View 4 publications 4 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          -
          View 6 publications 6 publications
          Product Specifications

          Species Reactivity

          Bovine, Human

          Published species

          Bovine, Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          HEC7

          Immunogen

          Human PECAM-1

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_223516

          Product Specific Information

          MA3100 targets CD31 in ICC/IF and ACS applications and shows reactivity with Human and Bovine samples.



          The MA3100 immunogen is human PECAM-1.



          MA3100 detects CD31 which has a predicted molecular weight of approximately 80 kDa.



          This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

          Target Information

          CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1

          View more View less

          Gene Aliases: BOS_19340; CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

          View more View less

          UniProt ID: (Human) P16284

          View more View less

          Entrez Gene ID: (Bovine) 282303, (Human) 5175

          View more View less

          Function(s)
          protein binding
          Process(es)
          cell adhesion platelet degranulation phagocytosis signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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