Invitrogen

CD31 Monoclonal Antibody (SP38)

1 Published Figure

12 References

View all (297) CD31 (PECAM-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD31 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

CD31 Antibody (MA5-16337) in IHC (P)

Immunohistochemical (paraffin) analysis of CD31 using anti-CD31 Monoclonal Antibody (Product # MA5-16337) in Tonsil Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:50.

CD31 Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-16337

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP38

Immunogen

Synthetic peptide corresponding to C-terminus of mouse CD31

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant/TBS, pH 7.5, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2537856

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting the pericyte antigen DLK1 with an alpha type-1 polarized dendritic cell vaccine results in tumor vascular modulation and protection against colon cancer progression.

Frontiers in immunology

McCormick AL,Anderson TS,Daugherity EA,Okpalanwaka IF,Smith SL,Appiah D,Lowe DB

MA5-16337 was used in Immunohistochemistry (Paraffin) to further interrogate immune-mediated mechanisms of protection in order to help devise efficacious αDC1-based strategies for patients with CRC.

Mon Oct 23 00:00:00 UTC 2023

Reposition of the anti-inflammatory drug diacerein in an in-vivo colorectal cancer model.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society

Abdel-Latif RT,Wadie W,Abdel-Mottaleb Y,Abdallah DM,El-Maraghy NN,El-Abhar HS

Published figure using CD31 monoclonal antibody (Product # MA5-16337) in Immunohistochemistry

Sat Jan 01 00:00:00 UTC 2022

Cellular architecture of human brain metastases.

Cell

Gonzalez H,Mei W,Robles I,Hagerling C,Allen BM,Hauge Okholm TL,Nanjaraj A,Verbeek T,Kalavacherla S,van Gogh M,Georgiou S,Daras M,Phillips JJ,Spitzer MH,Roose JP,Werb Z

MA5-16337 was used in Immunohistochemistry to present an integrative analysis of >100,000 malignant and non-malignant cells from 15 human parenchymal BrMs, generated by single-cell transcriptomics, mass cytometry, and complemented with mouse model- and in silico approaches.

Thu Feb 17 00:00:00 UTC 2022

Astrocytic trans-Differentiation Completes a Multicellular Paracrine Feedback Loop Required for Medulloblastoma Tumor Growth.

Cell

Yao M,Ventura PB,Jiang Y,Rodriguez FJ,Wang L,Perry JSA,Yang Y,Wahl K,Crittenden RB,Bennett ML,Qi L,Gong CC,Li XN,Barres BA,Bender TP,Ravichandran KS,Janes KA,Eberhart CG,Zong H

MA5-16337 was used in Immunohistochemistry to reveal an evolutionary process that produces a multi-lateral network within the TME of medulloblastoma: a fraction of tumor cells trans-differentiate into TuAstrocytes, which, in turn, produce IL-4 that stimulates microglia to produce IGF1 to promote tumor progression.

Thu Feb 06 00:00:00 UTC 2020

Defining the role of pulmonary endothelial cell heterogeneity in the response to acute lung injury.

eLife

Niethamer TK,Stabler CT,Leach JP,Zepp JA,Morley MP,Babu A,Zhou S,Morrisey EE

MA5-16337 was used in Immunohistochemistry to identify that different subtypes of endothelial cells exist in capillaries, and that some may be able to perform slightly different jobs during lung recovery.

Mon Feb 24 00:00:00 UTC 2020

Spirulina improves non-alcoholic steatohepatitis, visceral fat macrophage aggregation, and serum leptin in a mouse model of metabolic syndrome.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver

Fujimoto M,Tsuneyama K,Fujimoto T,Selmi C,Gershwin ME,Shimada Y

MA5-16337 was used in immunohistochemistry to study the beneficial effects of spirulina on macrophages and metabolic parameters in a murine model of metabolic syndrome

Sat Sep 01 00:00:00 UTC 2012

Hyperglycemia limits experimental aortic aneurysm progression.

Journal of vascular surgery

Miyama N,Dua MM,Yeung JJ,Schultz GM,Asagami T,Sho E,Sho M,Dalman RL

MA5-16337 was used in immunohistochemistry to study the effect of hyperglycemia on the progression of experimental aortic aneurysm

Fri Oct 01 00:00:00 UTC 2010

Eukaryotic expression of extracellular ligand binding domains of murine Tie-2 and its anti-angiogenesis effect in SGC-7901 cell lines.

Journal of gastroenterology and hepatology

Yang H,Yang K,Hu JK,Tang H,Zhang B,Chen ZX,Wang YJ,Chen JP

MA5-16337 was used in immunohistochemistry to study the anti-angiogenic effects on a gastric cancer cell line of the recombinantly expressed extracellular ligand binding domains of murine Tie-2

Mon Feb 01 00:00:00 UTC 2010

Correlation between scaffold in vivo biocompatibility and in vitro cell compatibility using mesenchymal and mononuclear cell cultures.

Cell biology and toxicology

Seo YK,Yoon HH,Park YS,Song KY,Lee WS,Park JK

MA5-16337 was used in immunohistochemistry to comapre silk and polyglycolic acid scaffold biocompatibility using mesenchymal and mononuclear cell cultures

Thu Oct 01 00:00:00 UTC 2009

Sorafenib and rapamycin induce growth suppression in mouse models of hepatocellular carcinoma.

Journal of cellular and molecular medicine

Huynh H,Ngo VC,Koong HN,Poon D,Choo SP,Thng CH,Chow P,Ong HS,Chung A,Soo KC

MA5-16337 was used in immunohistochemistry to study the antitumour activity and mechanisms of action of sorafenib against hepatocellular carcinoma xenografts

Sat Aug 01 00:00:00 UTC 2009

Brivanib alaninate, a dual inhibitor of vascular endothelial growth factor receptor and fibroblast growth factor receptor tyrosine kinases, induces growth inhibition in mouse models of human hepatocellular carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Huynh H,Ngo VC,Fargnoli J,Ayers M,Soo KC,Koong HN,Thng CH,Ong HS,Chung A,Chow P,Pollock P,Byron S,Tran E

MA5-16337 was used in immunohistochemistry to study the ability of a dual inhibitor of VEGF receptor and FGF receptor tyrosine kinases to inhibit growth in hepatocellular carcinoma

Wed Oct 01 00:00:00 UTC 2008

A central role for hepatocyte growth factor in adipose tissue angiogenesis.

American journal of physiology. Endocrinology and metabolism

Bell LN,Cai L,Johnstone BH,Traktuev DO,March KL,Considine RV

MA5-16337 was used in immunohistochemistry to study adipose tissue angiogenesis and the role of HGF

Fri Feb 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

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Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

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UniProt ID: (Human) P16284

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Entrez Gene ID: (Human) 5175

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Function(s)

protein binding

Process(es)

platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development

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CD31 Monoclonal Antibody (SP38)

CD31 Antibody (MA5-16337) in IHC (P)

Product Details

MA5-16337

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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