Invitrogen

CD31 Monoclonal Antibody (TLD-3A12)

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CD31 Antibody (MA1-81051) in WB

Western blot was performed using Anti-CD31 Monoclonal Antibody (TLD-3A12) (Product # MA1-81051) and a 130kDa band corresponding to Platelet endothelial cell adhesion molecule was observed across cell lines tested. Membrane enriched extracts (40 µg lysate) of HUVEC (Lane 1), HEL 92.1.7 (Lane 2), Jurkat (Lane 3), MDA-MB-231 (Lane 4), THP-1 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blot... View More

CD31 Antibody in Immunocytochemistry (ICC/IF)

CD31 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD31 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Platelet endothelial cell adhesion molecule was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 using Anti-CD31 Monoclonal Antibody (TLD-3A12) (Product # MA1-81051) in Western Blot (Ref :https://www.nature.com/articles/onc2013214). {RE}

Product Details

MA1-81051

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

1:10-1:100

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Rhesus monkey, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TLD-3A12

Immunogen

Activated, Lewis rat derived microglial cells.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_928154

Product Specific Information

Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions.

Aging

Deng Z,Zhou L,Wang Y,Liao S,Huang Y,Shan Y,Tan S,Zeng Q,Peng L,Huang H,Lu Z

MA1-81051 was used in Immunohistochemistry-immunofluorescence to reveal a new axis linking a high salt diet (HSD) to increased cerebral microvascular permeability through a VEGF-initiated inflammatory response.

Mon Jun 22 00:00:00 UTC 2020

Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions.

Aging

Deng Z,Zhou L,Wang Y,Liao S,Huang Y,Shan Y,Tan S,Zeng Q,Peng L,Huang H,Lu Z

Mon Jun 22 00:00:00 UTC 2020

Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages.

BMC molecular and cell biology

Bhandari S,Li R,Simón-Santamaría J,McCourt P,Johansen SD,Smedsrød B,Martinez-Zubiaurre I,Sørensen KK

MA1-81051 was used in Immunocytochemistry to support complementary and partly overlapping scavenging and immune functions of LSECs and KCs.

Fri Nov 27 00:00:00 UTC 2020

Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages.

BMC molecular and cell biology

Bhandari S,Li R,Simón-Santamaría J,McCourt P,Johansen SD,Smedsrød B,Martinez-Zubiaurre I,Sørensen KK

MA1-81051 was used in Immunocytochemistry to support complementary and partly overlapping scavenging and immune functions of LSECs and KCs.

Fri Nov 27 00:00:00 UTC 2020

YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT.

Stem cell research & therapy

Lin F,Zeng Z,Song Y,Li L,Wu Z,Zhang X,Li Z,Ke X,Hu X

Published figure using CD31 monoclonal antibody (Product # MA1-81051) in Western Blot

Fri Aug 23 00:00:00 UTC 2019

Identifying blood-brain-barrier selective single-chain antibody fragments.

Biotechnology journal

Jones AR,Stutz CC,Zhou Y,Marks JD,Shusta EV

MA1-81051 was used in immunohistochemistry to identify antibodies that bind antigens present at the blood brain barrier

Thu May 01 00:00:00 UTC 2014

Identifying blood-brain-barrier selective single-chain antibody fragments.

Biotechnology journal

Jones AR,Stutz CC,Zhou Y,Marks JD,Shusta EV

MA1-81051 was used in immunohistochemistry to identify antibodies that bind antigens present at the blood brain barrier

Thu May 01 00:00:00 UTC 2014

Erythropoietin protects against doxorubicin-induced heart failure.

American journal of physiology. Heart and circulatory physiology

Ammar HI,Saba S,Ammar RI,Elsayed LA,Ghaly WB,Dhingra S

MA1-81051 was used in immunohistochemistry to study the ability of erythropoeitin to protect against heart failure induced by treatment with the cancer drug doxorubicin

Thu Dec 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

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Gene Aliases: CD31; Pecam; Pecam1

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UniProt ID: (Rat) Q3SWT0

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Entrez Gene ID: (Rhesus monkey) 718302, (Rat) 29583

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Function(s)

protein binding

Process(es)

positive regulation of leukocyte migration phagocytosis cell adhesion Rho protein signal transduction regulation of cell migration negative regulation of actin filament polymerization response to cytokine negative regulation of GTPase activity wound healing positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of peptidyl-tyrosine phosphorylation diapedesis glomerular endothelium development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD31 Monoclonal Antibody (TLD-3A12)

CD31 Antibody (MA1-81051) in WB

Product Details

MA1-81051

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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