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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD31 (PECAM-1) Antibodies

          Invitrogen

          CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright™ 645, eBioscience™

          77 Published Figures
          68 References
          View all (297) CD31 (PECAM-1) antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite CD31 (PECAM-1) Monoclonal Antibody (390), Super Bright™ 645, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (77)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 78

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          CD31 (PECAM-1) Antibody (64-0311-82) in Flow

          BALB/c mouse bone marrow cells were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, Super Bright 645 (Product # 64-4031-82) (left) or 0.125 µg of CD31 Monoclonal Antibody, Super Bright 645 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Western Blot (WB)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)

          Product Details

          64-0311-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 13 publications 13 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 10 publications 10 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 43 publications 43 publications
          Product Specifications

          Species Reactivity

          Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          390

          Conjugate

          Super Bright™ 645 Super Bright™ 645 Super Bright™ 645
          • Unconjugated
          • APC 
          • Biotin 
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant Violet 421
          • Brilliant Violet 711
          • eFluor 450
          • FITC
          • Functional Grade
          • NovaFluor Blue 660-120S
          • NovaFluor Red 685
          • NovaFluor Red 710
          • NovaFluor Red 725
          • NovaFluor Red 755
          • NovaFluor Yellow 610
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Request custom conjugation

          Excitation/Emission Max

          414/645 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2717091

          Product Specific Information

          Description: The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

          Applications Reported: This 390 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This 390 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

          When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

          Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

          Target Information

          CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

          View more View less

          Gene Aliases: C85791; Cd31; Pecam; PECAM-1; Pecam1

          View more View less

          UniProt ID: (Mouse) Q08481

          View more View less

          Entrez Gene ID: (Mouse) 18613

          View more View less

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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