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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD31 (PECAM-1) Antibodies

          Invitrogen

          CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Biotin, eBioscience™

          Advanced Verification
          45 Published Figures
          58 References
          View all (297) CD31 (PECAM-1) antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Biotin, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (45)
          • Advanced Verification (1)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 47

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          CD31 (PECAM-1) Antibody (13-0319-82) in Flow

          Surface staining of normal human peripheral blood cells with Anti-Human CD31 (PECAM-1) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD31 (PECAM-1) Antibody in Immunocytochemistry (ICC/IF)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD31 (PECAM-1) Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Immunohistochemistry (IHC)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)
          CD31 (PECAM-1) Antibody

          Product Details

          13-0319-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 7 publications 7 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 41 publications 41 publications

          Functional Assay (Functional)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          WM-59 (WM59)

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • APC 
          • APC-eFluor 780
          • eFluor 450
          • FITC
          • Functional Grade
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_466423

          Product Specific Information

          Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

          Applications Reported: The WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The WM-59 (WM59) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD31; CD31 antigen; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

          View more View less

          Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

          View more View less

          UniProt ID: (Human) P16284

          View more View less

          Entrez Gene ID: (Human) 5175

          View more View less

          Function(s)
          protein binding
          Process(es)
          platelet degranulation phagocytosis cell adhesion signal transduction cell recognition extracellular matrix organization leukocyte migration diapedesis glomerular endothelium development
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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