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Invitrogen
CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Functional Grade, eBioscience™
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FIGURE: 1 / 47
CD324 (E-Cadherin) Antibody (16-3249-82) in ICC/IF
Immunofluorescence analysis of E-cadherin was performed using 90% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1) (Product # 16-3249-82) at 5 µg/mL in 0.1% BSA, incubated at 4 d... View More
Product Details
16-3249-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
DECMA-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10734213
Product Specific Information
Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton. Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.
E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.
The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.
Applications Reported: This DECMA-1 antibody has been reported for use in functional blocking assays and flow cytometric analysis.
Applications Tested: This DECMA-1 antibody has been tested by flow cytometric analysis of cell lines. Optimal staining is achieved by intracellular staining as protein turnover can result in variable surface staining. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin
Gene Aliases: AA960649; Arc-1; Cadherin-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO; Uvomorulin
UniProt ID: (Dog) F1PAA9, (Human) P12830, (Mouse) P09803
Entrez Gene ID: (Dog) 442858, (Human) 999, (Mouse) 12550
glycoprotein binding
calcium ion binding
protein binding
beta-catenin binding
ankyrin binding
GTPase activating protein binding
gamma-catenin binding
cell adhesion molecule binding
cadherin binding involved in cell-cell adhesion
protein phosphatase binding
protein domain specific binding
metal ion binding
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
sensory perception of sound
single organismal cell-cell adhesion
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
protein metabolic process
negative regulation of cell-cell adhesion
regulation of protein localization
regulation of water loss via skin
adherens junction organization
positive regulation of transcription factor import into nucleus
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of transcription, DNA-templated
regulation of epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein homooligomerization
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
bicellular tight junction assembly
cellular response to amino acid stimulus
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
establishment of protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cochlea development
regulation of protein localization to cell surface
regulation of neuron migration
response to toxic substance
pituitary gland development
extracellular matrix disassembly
extracellular matrix organization
neuron projection development
response to drug
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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