Invitrogen

CD326 (EpCAM) Monoclonal Antibody (1B7), eBioscience™

CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 33

CD326 (EpCAM) Antibody (14-9326-82) in ICC/IF

Immunofluorescence analysis of EpCAM was performed using HT-29 and BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EpCAM Mouse Monoclonal Antibody (Product # 14-9326-82) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant S... View More

CD326 (EpCAM) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD326 (EpCAM) Antibody in Flow Cytometry (Flow)

CD326 (EpCAM) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD326 (EpCAM) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)

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Product Details

14-9326-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

View 5 publications 5 publications

Flow Cytometry (Flow)

0.25 µg/test

View 22 publications 22 publications

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1B7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_795876

Product Specific Information

Description: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.

Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis, immunohistochemistry of formalin-fixed paraffin embedded or frozen tissue sections, and immunoprecipitation. The 1B7 antibody has also been found useful for immunoblotting, and recognizes a protein of approximately 40 kD under non-reducing conditions.

Applications Tested: This 1B7 antibody has been tested by flow cytometric analysis of A549 human lung carcinoma cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 1B7 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characteristics of Extracellular Vesicles from a High-Grade Serous Ovarian Cancer Cell Line Derived from a Platinum-Resistant Patient as a Potential Tool for Aiding the Prediction of Responses to Chemotherapy.

Pharmaceuticals (Basel, Switzerland)

Černe K,Kelhar N,Resnik N,Herzog M,Vodnik L,Veranič P,Kobal B

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Tue Jun 20 00:00:00 UTC 2023

Improved platform for breast cancer circulating tumor cell enrichment and characterization with next-generation sequencing technology.

American journal of cancer research

Mihalcioiu C,Li J,Badescu D,Camirand A,Kremer N,Bertos N,Omeroglu A,Sebag M,Di Battista J,Park M,Ragoussis J,Kremer R

14-9326-82 was used in Immunocytochemistry-immunofluorescence to develop a platform based on the large-scale isolation of CTCs by apheresis, allowing us to collect CTCs in large numbers, which were preserved live in liquid nitrogen for further characterization.

Tue Feb 14 00:00:00 UTC 2023

Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.

International journal of cancer

Klusa D,Lohaus F,Franken A,Baumbach M,Cojoc M,Dowling P,Linge A,Offermann A,Löck S,Hušman D,Rivandi M,Polzer B,Freytag V,Lange T,Neubauer H,Kücken M,Perner S,Hölscher T,Dubrovska A,Krause M,Kurth I,Baumann M,Peitzsch C

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2023

A combination therapy of bortezomib, CXCR4 inhibitor, and checkpoint inhibitor is effective in cholangiocarcinoma in vivo.

iScience

Li L,Zhou Y,Zhang Y,Hu H,Mao HQ,Selaru FM

14-9326-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to find that the inhibition of CXCR4 allowed bortezomib to sensitize CCA to anti-PD1 treatment, with a significant reduction of tumor burden and long-term overall survival.

Fri Mar 17 00:00:00 UTC 2023

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Sat Oct 01 00:00:00 UTC 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Mon Nov 14 00:00:00 UTC 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Mon Nov 14 00:00:00 UTC 2022

The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay.

Molecular therapy. Methods & clinical development

Hirai H,Liang X,Sun Y,Zhang Y,Zhang J,Chen YE,Mou H,Zhao Y,Xu J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Thu Mar 10 00:00:00 UTC 2022

Selection of Cancer Stem Cell-Targeting Agents Using Bacteriophage Display.

Methods in molecular biology (Clifton, N.J.)

Prater AR,Deutscher SL

14-9326-82 was used in Flow cytometry/Cell sorting to discuss in detail how to isolate a CSC like population of cells from an aggressive cancer cell line, perform in vivo and in vitro phage display selections against the CSCs, and then characterize the resulting phage/peptides for further use as a diagnostic and therapeutic tool.

Wed Mar 30 00:00:00 UTC 2022

Parathyroid Hormone-Related Protein Inhibition Blocks Triple-Negative Breast Cancer Expansion in Bone Through Epithelial to Mesenchymal Transition Reversal.

JBMR plus

Li J,Camirand A,Zakikhani M,Sellin K,Guo Y,Luan X,Mihalcioiu C,Kremer R

14-9326-82 was used in Immunocytochemistry-immunoflourescence to conclude that PTHrP is a targetable EMT molecular driver and suggest that its pharmacological blockade can provide a potential therapeutic approach against established TNBC-derived skeletal lesions.

Wed Jun 01 00:00:00 UTC 2022

Long-Term Characteristics of Human-Derived Biliary Organoids under a Single Continuous Culture Condition.

Cells

Aktas RG,Karski M,Issac B,Sun L,Rockowitz S,Sliz P,Vakili K

14-9326-82 was used in Immunohistochemistry to determine the feasibility of establishing organoids from various pediatric liver diseases and characterize the long-term evolution of cholangiocyte organoids (chol-orgs) under a single continuous culture condition.

Sun Nov 27 00:00:00 UTC 2022

GSDMB is increased in IBD and regulates epithelial restitution/repair independent of pyroptosis.

Cell

Rana N,Privitera G,Kondolf HC,Bulek K,Lechuga S,De Salvo C,Corridoni D,Antanaviciute A,Maywald RL,Hurtado AM,Zhao J,Huang EH,Li X,Chan ER,Simmons A,Bamias G,Abbott DW,Heaney JD,Ivanov AI,Pizarro TT

14-9326-82 was used in Immunohistochemistry to reveal lack of inherent GSDMB-dependent pyroptosis in activated epithelial cells and organoids but instead point to increased proliferation and migration during in vitro wound closure, which arrests in GSDMB-deficient cells that display hyper-adhesiveness and enhanced formation of vinculin-based focal adhesions dependent on PDGF-A-mediated FAK phosphorylation.

Thu Jan 20 00:00:00 UTC 2022

Human Nasal and Lung Tissues Infected Ex Vivo with SARS-CoV-2 Provide Insights into Differential Tissue-Specific and Virus-Specific Innate Immune Responses in the Upper and Lower Respiratory Tract.

Journal of virology

Alfi O,Yakirevitch A,Wald O,Wandel O,Izhar U,Oiknine-Djian E,Nevo Y,Elgavish S,Dagan E,Madgar O,Feinmesser G,Pikarsky E,Bronstein M,Vorontsov O,Jonas W,Ives J,Walter J,Zakay-Rones Z,Oberbaum M,Panet A,Wolf DG

14-9326-82 was used in Immunohistochemistry to investigate viral susceptibility and early patterns of local mucosal innate immune response in the authentic milieu of the human respiratory tract. SARS-CoV-2 productively infected the nasal turbinate tissues, predominantly targeting respiratory epithelial cells, with a rapid increase in tissue-associated viral subgenomic mRNA and secretion of infectious viral progeny.

Thu Jun 24 00:00:00 UTC 2021

EMP3 negatively modulates breast cancer cell DNA replication, DNA damage repair, and stem-like properties.

Cell death & disease

Zhou K,Sun Y,Dong D,Zhao C,Wang W

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Sun Sep 12 00:00:00 UTC 2021

Helicobacter pylori promote inflammation and host defense through the cagA-dependent activation of mTORC1.

Journal of cellular physiology

Feng GJ,Chen Y,Li K

14-9326-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to support H. pylori promote gastritis and inhibit bacterial colonization through the cagA-dependent activation of mTORC1.

Tue Dec 01 00:00:00 UTC 2020

Helicobacter pylori promote inflammation and host defense through the cagA-dependent activation of mTORC1.

Journal of cellular physiology

Feng GJ,Chen Y,Li K

Tue Dec 01 00:00:00 UTC 2020

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Cell death & disease

Sun Y,Wang Z,Na L,Dong D,Wang W,Zhao C

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Sat Dec 12 00:00:00 UTC 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Wed Jan 08 00:00:00 UTC 2020

Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nature communications

Nguyen QH,Pervolarakis N,Blake K,Ma D,Davis RT,James N,Phung AT,Willey E,Kumar R,Jabart E,Driver I,Rock J,Goga A,Khan SA,Lawson DA,Werb Z,Kessenbrock K

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Wed May 23 00:00:00 UTC 2018

A genome editing approach to study cancer stem cells in human tumors.

EMBO molecular medicine

Cortina C,Turon G,Stork D,Hernando-Momblona X,Sevillano M,Aguilera M,Tosi S,Merlos-Suárez A,Stephan-Otto Attolini C,Sancho E,Batlle E

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2017

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level.

Stem cell reports

Pode-Shakked N,Gershon R,Tam G,Omer D,Gnatek Y,Kanter I,Oriel S,Katz G,Harari-Steinberg O,Kalisky T,Dekel B

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Tue Jul 11 00:00:00 UTC 2017

Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level.

Stem cell reports

Pode-Shakked N,Gershon R,Tam G,Omer D,Gnatek Y,Kanter I,Oriel S,Katz G,Harari-Steinberg O,Kalisky T,Dekel B

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Tue Jul 11 00:00:00 UTC 2017

Triple-negative breast cancer targeting and killing by EpCAM-directed, plasmonically active nanodrug systems.

NPJ precision oncology

Jenkins SV,Nima ZA,Vang KB,Kannarpady G,Nedosekin DA,Zharov VP,Griffin RJ,Biris AS,Dings RPM

14-9326 was used in Flow cytometry/Cell sorting to engineer a nanodrug delivery system consisting of silver-coated golf nanorods targeting epithelial cell adhesion/activating molecule and loaded with doxorubicin.

Thu Oct 01 00:00:00 UTC 2020

Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells.

Proceedings of the National Academy of Sciences of the United States of America

Cheung SK,Chuang PK,Huang HW,Hwang-Verslues WW,Cho CH,Yang WB,Shen CN,Hsiao M,Hsu TL,Chang CF,Wong CH

14932682 was used in flow cytometry to find that breast cancer stem cells isolated with CD44(+)CD24(-/lo)SSEA-3(+) or ESA(hi)PROCR(hi)SSEA-3(+) markers have higher tumorigenicity than those with conventional markers

Tue Jan 26 00:00:00 UTC 2016

Clostridium perfringens enterotoxin C-terminal domain labeled to fluorescent dyes for in vivo visualization of micrometastatic chemotherapy-resistant ovarian cancer.

International journal of cancer

Cocco E,Shapiro EM,Gasparrini S,Lopez S,Schwab CL,Bellone S,Bortolomai I,Sumi NJ,Bonazzoli E,Nicoletti R,Deng Y,Saltzman WM,Zeiss CJ,Centritto F,Black JD,Silasi DA,Ratner E,Azodi M,Rutherford TJ,Schwartz PE,Pecorelli S,Santin AD

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Immunofluorescence

Tue Dec 01 00:00:00 UTC 2015

The isolation and characterization of CTC subsets related to breast cancer dormancy.

Scientific reports

Vishnoi M,Peddibhotla S,Yin W,T Scamardo A,George GC,Hong DS,Marchetti D

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Thu Dec 03 00:00:00 UTC 2015

A lung-on-a-chip array with an integrated bio-inspired respiration mechanism.

Lab on a chip

Stucki AO,Stucki JD,Hall SR,Felder M,Mermoud Y,Schmid RA,Geiser T,Guenat OT

14-9326 was used in Flow cytometry/Cell sorting to report a novel lung-on-a-chip that mimics the pulmonary parenchymal environment, including the alveolar barrier and the 3D cyclic strain induced by breathing movements.

Sat Mar 07 00:00:00 UTC 2015

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Tue Jul 15 00:00:00 UTC 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Fri Feb 01 00:00:00 UTC 2013

Estrogen and progesterone together expand murine endometrial epithelial progenitor cells.

Stem cells (Dayton, Ohio)

Janzen DM,Cheng D,Schafenacker AM,Paik DY,Goldstein AS,Witte ON,Jaroszewicz A,Pellegrini M,Memarzadeh S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Immunohistochemistry

Mon Apr 01 00:00:00 UTC 2013

Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.

Stem cells (Dayton, Ohio)

Paik DY,Janzen DM,Schafenacker AM,Velasco VS,Shung MS,Cheng D,Huang J,Witte ON,Memarzadeh S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Thu Nov 01 00:00:00 UTC 2012

Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.

Stem cells (Dayton, Ohio)

Paik DY,Janzen DM,Schafenacker AM,Velasco VS,Shung MS,Cheng D,Huang J,Witte ON,Memarzadeh S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Thu Nov 01 00:00:00 UTC 2012

Slow-cycling therapy-resistant cancer cells.

Stem cells and development

Moore N,Houghton J,Lyle S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 14-9326-82) in Flow Cytometry

Sun Jul 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Tumor-associated calcium signal transducer 1

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Gene Aliases: DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1

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UniProt ID: (Human) P16422

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Entrez Gene ID: (Human) 4072

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Function(s)

protein binding protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD326 (EpCAM) Monoclonal Antibody (1B7), eBioscience™

CD326 (EpCAM) Antibody (14-9326-82) in ICC/IF

Product Details

14-9326-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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