Invitrogen

CD326 (EpCAM) Monoclonal Antibody (1B7), PE-Cyanine7, eBioscience™

18 Published Figures

21 References

View all (272) EpCAM (CD326) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (18)

CD326 (EpCAM) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 19

CD326 (EpCAM) Antibody (25-9326-42) in Flow

Staining of A549 cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD326 (EpCAM) PE-Cyanine7 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

25-9326-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1B7

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2573542

Product Specific Information

Description: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.

Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1B7 antibody has been pre-titrated and tested by flow cytometric analysis of the A549 cell line. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Characteristics of Extracellular Vesicles from a High-Grade Serous Ovarian Cancer Cell Line Derived from a Platinum-Resistant Patient as a Potential Tool for Aiding the Prediction of Responses to Chemotherapy.

Pharmaceuticals (Basel, Switzerland)

Černe K,Kelhar N,Resnik N,Herzog M,Vodnik L,Veranič P,Kobal B

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Tue Jun 20 00:00:00 UTC 2023

Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.

International journal of cancer

Klusa D,Lohaus F,Franken A,Baumbach M,Cojoc M,Dowling P,Linge A,Offermann A,Löck S,Hušman D,Rivandi M,Polzer B,Freytag V,Lange T,Neubauer H,Kücken M,Perner S,Hölscher T,Dubrovska A,Krause M,Kurth I,Baumann M,Peitzsch C

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2023

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

25-9326-42 was used in Flow cytometry/Cell sorting to provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond.

Sat Oct 01 00:00:00 UTC 2022

The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.

Molecular nutrition & food research

Doerfler R,Melamed JR,Whitehead KA

25-9326-42 was used in Flow cytometry/Cell sorting to provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond.

Sat Oct 01 00:00:00 UTC 2022

Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer.

Scientific reports

Le UQ,Chen N,Balasenthil S,Lurie E,Yang F,Liu S,Rubin L,Solis Soto LM,Raso MG,Batra H,Sahin AA,Wistuba II,Killary AM

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Mon Nov 14 00:00:00 UTC 2022

The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay.

Molecular therapy. Methods & clinical development

Hirai H,Liang X,Sun Y,Zhang Y,Zhang J,Chen YE,Mou H,Zhao Y,Xu J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Thu Mar 10 00:00:00 UTC 2022

Functional patient-derived organoid screenings identify MCLA-158 as a therapeutic EGFR × LGR5 bispecific antibody with efficacy in epithelial tumors.

Nature cancer

Herpers B,Eppink B,James MI,Cortina C,Cañellas-Socias A,Boj SF,Hernando-Momblona X,Glodzik D,Roovers RC,van de Wetering M,Bartelink-Clements C,Zondag-van der Zande V,Mateos JG,Yan K,Salinaro L,Basmeleh A,Fatrai S,Maussang D,Lammerts van Bueren JJ,Chicote I,Serna G,Cabellos L,Ramírez L,Nuciforo P,Salazar R,Santos C,Villanueva A,Stephan-Otto Attolini C,Sancho E,Palmer HG,Tabernero J,Stratton MR,de Kruif J,Logtenberg T,Clevers H,Price LS,Vries RGJ,Batlle E,Throsby M

25-9326-42 was used in Flow cytometry/Cell sorting to describe a large-scale functional screen of dual-targeting bispecific antibodies (bAbs) on a heterogeneous colorectal cancer PDO biobank and paired healthy colonic mucosa samples.

Fri Apr 01 00:00:00 UTC 2022

EMP3 negatively modulates breast cancer cell DNA replication, DNA damage repair, and stem-like properties.

Cell death & disease

Zhou K,Sun Y,Dong D,Zhao C,Wang W

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Sun Sep 12 00:00:00 UTC 2021

A molecular cell atlas of the human lung from single-cell RNA sequencing.

Nature

Travaglini KJ,Nabhan AN,Penland L,Sinha R,Gillich A,Sit RV,Chang S,Conley SD,Mori Y,Seita J,Berry GJ,Shrager JB,Metzger RJ,Kuo CS,Neff N,Weissman IL,Quake SR,Krasnow MA

25-9326 was used in Flow cytometry/Cell sorting to create an extensive cell atlas of the human lung by droplet- and plate-based single-cell RNA sequencing of approximately 75,000 human cells across all lung tissue compartments and circulating blood, combined with a multi-pronged cell annotation approach.

Sun Nov 01 00:00:00 UTC 2020

Chemotherapeutic Stress Influences Epithelial-Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer.

International journal of molecular sciences

Li X,Strietz J,Bleilevens A,Stickeler E,Maurer J

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Wed Jan 08 00:00:00 UTC 2020

FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.

Cell death & disease

Sun Y,Wang Z,Na L,Dong D,Wang W,Zhao C

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Sat Dec 12 00:00:00 UTC 2020

The hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.

Breast cancer research : BCR

Kim H,Lin Q,Glazer PM,Yun Z

25-9326 was used in Flow cytometry/Cell sorting to suggest that the hypoxic TME promotes malignant progression and therapy resistance via induction, selection and preferential maintenance of CSC-like cells.

Tue Mar 06 00:00:00 UTC 2018

Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nature communications

Nguyen QH,Pervolarakis N,Blake K,Ma D,Davis RT,James N,Phung AT,Willey E,Kumar R,Jabart E,Driver I,Rock J,Goga A,Khan SA,Lawson DA,Werb Z,Kessenbrock K

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Wed May 23 00:00:00 UTC 2018

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods.

Disease markers

Fina E,Necchi A,Bottelli S,Reduzzi C,Pizzamiglio S,Iacona C,Daidone MG,Verderio P,Cappelletti V

25-9326 was used in Flow cytometry/Cell sorting to analyse CTC enrichment by AdnaTest ProstateCancerSelect (AT) and ScreenCell®Cyto (SC) kits, combined with identification by EPCAM, MUC1, and ERBB2 expression and by cytological criteria.

Mon Mar 27 00:00:00 UTC 2017

Evidence of In Vitro Preservation of Human Nephrogenesis at the Single-Cell Level.

Stem cell reports

Pode-Shakked N,Gershon R,Tam G,Omer D,Gnatek Y,Kanter I,Oriel S,Katz G,Harari-Steinberg O,Kalisky T,Dekel B

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Tue Jul 11 00:00:00 UTC 2017

Characterization of a switchable chimeric antigen receptor platform in a pre-clinical solid tumor model.

Oncoimmunology

Pishali Bejestani E,Cartellieri M,Bergmann R,Ehninger A,Loff S,Kramer M,Spehr J,Dietrich A,Feldmann A,Albert S,Wermke M,Baumann M,Krause M,Bornhäuser M,Ehninger G,Bachmann M,von Bonin M

25-9326 was used in Flow cytometry/Cell sorting to illustrate that the UniCAR platform mediates potent anti-tumor activity in a relevant in vitro and in vivo solid tumor model.

Tue Jan 12 00:00:00 UTC 2021

The isolation and characterization of CTC subsets related to breast cancer dormancy.

Scientific reports

Vishnoi M,Peddibhotla S,Yin W,T Scamardo A,George GC,Hong DS,Marchetti D

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Thu Dec 03 00:00:00 UTC 2015

NDY1/KDM2B functions as a master regulator of polycomb complexes and controls self-renewal of breast cancer stem cells.

Cancer research

Kottakis F,Foltopoulou P,Sanidas I,Keller P,Wronski A,Dake BT,Ezell SA,Shen Z,Naber SP,Hinds PW,McNiel E,Kuperwasser C,Tsichlis PN

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Tue Jul 15 00:00:00 UTC 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Fri Feb 01 00:00:00 UTC 2013

Stem-like epithelial cells are concentrated in the distal end of the fallopian tube: a site for injury and serous cancer initiation.

Stem cells (Dayton, Ohio)

Paik DY,Janzen DM,Schafenacker AM,Velasco VS,Shung MS,Cheng D,Huang J,Witte ON,Memarzadeh S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Thu Nov 01 00:00:00 UTC 2012

Slow-cycling therapy-resistant cancer cells.

Stem cells and development

Moore N,Houghton J,Lyle S

Published figure using CD326 (EpCAM) monoclonal antibody (Product # 25-9326-42) in Flow Cytometry

Sun Jul 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Tumor-associated calcium signal transducer 1

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Gene Aliases: DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1

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UniProt ID: (Human) P16422

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Entrez Gene ID: (Human) 4072

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Function(s)

protein binding protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

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CD326 (EpCAM) Monoclonal Antibody (1B7), PE-Cyanine7, eBioscience™

CD326 (EpCAM) Antibody (25-9326-42) in Flow

Product Details

25-9326-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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