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          • Primary Antibodies ›
          • EpCAM (CD326) Antibodies

          Invitrogen

          CD326 (EpCAM) Monoclonal Antibody (G8.8), Biotin, eBioscience™

          94 Published Figures
          92 References
          View all (272) EpCAM (CD326) antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite CD326 (EpCAM) Monoclonal Antibody (G8.8), Biotin, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (94)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 95

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          CD326 (EpCAM) Antibody (13-5791-82) in Flow

          Staining of the TE-71 cell line with 0.125 µg of Rat IgG2a kappa Isotype Control Biotin (Product # 13-4321-82) (blue histogram) or 0.125 µg of Anti-Mouse CD326 (EpCAM) Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD326 (EpCAM) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunocytochemistry (ICC/IF)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Immunohistochemistry (IHC)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)
          CD326 (EpCAM) Antibody in Flow Cytometry (Flow)

          Product Details

          13-5791-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 15 publications 15 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 11 publications 11 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 59 publications 59 publications

          Affinity Purification (AP)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          G8.8

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • FITC
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1659713

          Product Specific Information

          Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. The antigen is being used as a target for immunotherapy treatment of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation. This epithelial glycoprotein is now recognized as having an important role in tumor biology.

          Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD326; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial glycoprotein 314; hEGP314; lymphocyte antigen 74; mEGP314; panepithelial glycoprotein 314; Protein 289A; Trop-1 protein; Tumor-associated calcium signal transducer 1

          View more View less

          Gene Aliases: CD326; EGP; EGP-2; Egp314; Ep-CAM; Epcam; EpCAM1; GA733-2; gp40; Ly74; Tacsd1; Tacstd1; TROP1

          View more View less

          UniProt ID: (Mouse) Q99JW5

          View more View less

          Entrez Gene ID: (Mouse) 17075

          View more View less

          Function(s)
          protein complex binding cadherin binding involved in cell-cell adhesion
          Process(es)
          positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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