Invitrogen

CD33 Monoclonal Antibody (HIM3-4), PE, eBioscience™

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CD33 Antibody (12-0339-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (open histogram) and Anti-Human CD33 PE (filled hisotgram). Cells in the monocyte population were used for analysis.

Product Image Gallery

CD33 Antibody — Generation of CD33 KO human HSPC in vitro (A) Full-length (20bp) and truncated (17-18bp guide RNAs (gRNAs) targeting CD33 were tested in for editing efficiency in a human AML cell line, Molm14, after electroporation with Cas9. (B) Human CD34+ cells were electroporated with Cas9 protein and truncated CD33-targeting gRNA4 (panel A), and surface CD33 protein expression was assessed by flow cytometry (left) and DNA mutations were quantified by SURVEYOR assay (right) following 7 days of in vitro myeloid differentiation culture. (C) Sanger sequencing of individual alleles reveals a high proportion of mutations containing a single A insertion (top); representative chromatogram is shown (bottom). (D) A single-stranded oligodeoxynucleotide (ssODN) was designed to contain 99bp homology arms around the gRNA cut site and the A insertion mutation (top). CD34+ cells were electroporated with Cas9 protein/gRNA and different concentrations of the ssODN, after which CD33 expression was assessed by flow cytometry (bottom left) following 7 days of culture. Addition of the ssODN increases the frequency of CD33 mutations in three different donors in three independent experiments (bottom right). (E) Experimental schema: Human CD34+ cells were electroporated with either Cas9 complexed with EMX1-gRNA (Control), or Cas9/CD33-gRNA4 and the ssODN (CD33 KO) and cultured for 7 days. (F) Cultured CD33 KO CD34+ cells have decreased levels of surface CD33 expression by flow cytometry compared to controls (n= {KO}

Product Details

12-0339-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIM3-4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10855031

Product Specific Information

Description: The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.

Applications Reported: The HIM3-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIM3-4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

Hua J,Jiao T,Qiao Y,Zhang S,Xiao T,Zhang Y,Yan J

12-0339-42 was used in Flow Cytometry /test) to find that HSA was beneficial to maintaining CD34+ cell expansion and short-term colony formation in vitro and optimizing multilineage reconstitution in immunodeficient mice in vivo.

Tue Jan 31 00:00:00 UTC 2023

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

Hua J,Jiao T,Qiao Y,Zhang S,Xiao T,Zhang Y,Yan J

Tue Jan 31 00:00:00 UTC 2023

Mesenchymal stem cells, as glioma exosomal immunosuppressive signal multipliers, enhance MDSCs immunosuppressive activity through the miR-21/SP1/DNMT1 positive feedback loop.

Journal of nanobiotechnology

Qiu W,Guo Q,Guo X,Wang C,Li B,Qi Y,Wang S,Zhao R,Han X,Du H,Zhao S,Pan Z,Fan Y,Wang Q,Gao Z,Li G,Xue H

Published figure using CD33 monoclonal antibody (Product # 12-0339-42) in Flow Cytometry

Sat Jul 22 00:00:00 UTC 2023

Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion.

Cancer cell

Su R,Dong L,Li Y,Gao M,Han L,Wunderlich M,Deng X,Li H,Huang Y,Gao L,Li C,Zhao Z,Robinson S,Tan B,Qing Y,Qin X,Prince E,Xie J,Qin H,Li W,Shen C,Sun J,Kulkarni P,Weng H,Huang H,Chen Z,Zhang B,Wu X,Olsen MJ,Müschen M,Marcucci G,Salgia R,Li L,Fathi AT,Li Z,Mulloy JC,Wei M,Horne D,Chen J

12-0339-42 was used in Flow Cytometry to demonstrate if fat mass and obesity-associated protein plays critical roles in cancer stem cell self-renewal and immune evasion.

Mon Jul 13 00:00:00 UTC 2020

Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia.

Cell

Kim MY,Yu KR,Kenderian SS,Ruella M,Chen S,Shin TH,Aljanahi AA,Schreeder D,Klichinsky M,Shestova O,Kozlowski MS,Cummins KD,Shan X,Shestov M,Bagg A,Morrissette JJD,Sekhri P,Lazzarotto CR,Calvo KR,Kuhns DB,Donahue RE,Behbehani GK,Tsai SQ,Dunbar CE,Gill S

Published figure using CD33 monoclonal antibody (Product # 12-0339-42) in Flow Cytometry

Thu May 31 00:00:00 UTC 2018

Activation of p53 in Immature Myeloid Precursor Cells Controls Differentiation into Ly6c⁺CD103⁺ Monocytic Antigen-Presenting Cells in Tumors.

Immunity

Sharma MD,Rodriguez PC,Koehn BH,Baban B,Cui Y,Guo G,Shimoda M,Pacholczyk R,Shi H,Lee EJ,Xu H,Johnson TS,He Y,Mergoub T,Venable C,Bronte V,Wolchok JD,Blazar BR,Munn DH

12-0339 was used in Flow cytometry/Cell sorting to show that during immunotherapy large numbers of cells expressing CD103 arose in murine tumours via direct differentiation of Ly6c+ monocytic precursors.

Tue Jan 16 00:00:00 UTC 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using CD33 monoclonal antibody (Product # 12-0339-42) in Flow Cytometry

Sun Jul 01 00:00:00 UTC 2018

KSRP specifies monocytic and granulocytic differentiation through regulating miR-129 biogenesis and RUNX1 expression.

Nature communications

Zhao H,Wang X,Yi P,Si Y,Tan P,He J,Yu S,Ren Y,Ma Y,Zhang J,Wang D,Wang F,Yu J

12-0339 was used in Flow cytometry/Cell sorting to elucidate how KH-Type Splicing Regulatory Protein, miR-129, and RUNX1 participate in a regulatory axis to control the outcome of myeloid differentiation.

Fri Nov 10 00:00:00 UTC 2017

Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Dabas R,Lee R,Servito MT,Dharmani-Khan P,Modi M,van Slyke T,Luider J,Durand C,Larratt L,Brandwein J,Morris D,Daly A,Khan FM,Storek J

Sun May 01 00:00:00 UTC 2016

Switching CAR T cells on and off: a novel modular platform for retargeting of T cells to AML blasts.

Blood cancer journal

Cartellieri M,Feldmann A,Koristka S,Arndt C,Loff S,Ehninger A,von Bonin M,Bejestani EP,Ehninger G,Bachmann MP

12-0339 was used in Flow cytometry/Cell sorting to describe a novel modular universal platform for retargeting of T cells to AML blasts, via switching CD19-specific chimeric antigen receptor engineered T cells on or off.

Fri Aug 12 00:00:00 UTC 2016

Oncogenic NRAS hyper-activates multiple pathways in human cord blood stem/progenitor cells and promotes myelomonocytic proliferation in vivo.

American journal of translational research

Wang T,Li C,Xia C,Dong Y,Yang D,Geng Y,Cai J,Zhang J,Zhang X,Wang J

12-0339 was used in Flow cytometry/Cell sorting to evaluate the effects of the NRASG12D mutation on human haematopoiesis and leukaemogenesis in vitro and in vivo.

Tue Dec 22 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: CD33; CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3

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Gene Aliases: CD33; p67; SIGLEC-3; SIGLEC3

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UniProt ID: (Human) P20138

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Entrez Gene ID: (Human) 945

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Function(s)

receptor activity protein binding carbohydrate binding

Process(es)

cell adhesion signal transduction cell-cell signaling negative regulation of cell proliferation regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD33 Monoclonal Antibody (HIM3-4), PE, eBioscience™

CD33 Antibody (12-0339-42) in Flow

Product Details

12-0339-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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