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          • Primary Antibodies ›
          • NKp46 (CD335) Antibodies
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          Invitrogen

          CD335 (NKp46) Monoclonal Antibody (29A1.4), Functional Grade, eBioscience™

          18 Published Figures
          49 References
          View all (59) NKp46 (CD335) antibodies

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          Cite CD335 (NKp46) Monoclonal Antibody (29A1.4), Functional Grade, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (18)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 19

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          CD335 (NKp46) Antibody (16-3351-38) in Flow

          Staining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (left) or 0.5 µg of Anti-Mouse CD335 (NKp46) Functional Grade Purified (right) followed by Anti-Rat IgG Biotin (Product # 13-4813-85), Streptavidin PE (Product # 12-4317-87), and finally Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82).Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD335 (NKp46) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)
          CD335 (NKp46) Antibody in Flow Cytometry (Flow)

          Product Details

          16-3351-38

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          1 µg/test
          View 47 publications 47 publications

          Functional Assay (Functional)

          Assay-Dependent
          View 1 publication 1 publication
          Product Specifications

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          29A1.4

          Conjugate

          Functional Grade Functional Grade Functional Grade
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant Violet 711
          • eFluor 450
          • eFluor 660
          • FITC
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Wet ice

          RRID

          AB_2865955

          Product Specific Information

          Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

          The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

          Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          CD335, also known as NKp46 or NCR1, is a member of the natural cytotoxicity receptor family, which includes NKp30 and NKp44. This type I transmembrane glycoprotein is expressed on NK cells and plays a crucial role in activating and expanding resting NK cells through interactions with dendritic cells. CD335 enhances the ability of NK cells to eliminate tumor and virus-infected cells, such as those infected by influenza. CD335 consists of two Ig-like domains and is related to leukocyte immunoglobulin-like receptors (LIR) and killer inhibitory receptors (KIR). It contributes to the increased efficiency of activated NK cells in mediating tumor cell lysis. Additionally, CD335 interacts with pancreatic beta cells during the development of type 1 diabetes, highlighting its involvement in both immune defense and autoimmune processes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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