Invitrogen

CD339 (Jagged 1) Monoclonal Antibody (HMJ1-29), PE, eBioscience™

CD339 (Jagged 1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD339 (Jagged 1) Antibody (12-3391-82) in Flow

Staining of CD8+CD11c+ C57BL/6 splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.5 µg of Anti-Mouse CD339 (Jagged 1) PE (purple histogram). Total viable cells were used for analysis.

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Product Details

12-3391-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HMJ1-29

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1963616

Product Specific Information

Description: This HMJ1-29 monoclonal antibody reacts with mouse Jagged-1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Jagged-1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Jagged-1 is expressed by thymic lymphoid and stromal cells, as well as macrophages and CD8+CD11c+ dendritic cells in the spleen.

The HMJ1-29 antibody is reported to crossreact to rat.

Applications Reported: This HMJ1-29 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HMJ1-29 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Regulatory T Cells in Skin Facilitate Epithelial Stem Cell Differentiation.

Cell

Ali N,Zirak B,Rodriguez RS,Pauli ML,Truong HA,Lai K,Ahn R,Corbin K,Lowe MM,Scharschmidt TC,Taravati K,Tan MR,Ricardo-Gonzalez RR,Nosbaum A,Bertolini M,Liao W,Nestle FO,Paus R,Cotsarelis G,Abbas AK,Rosenblum MD

12-3391 was used in Flow cytometry/Cell sorting to study the role of regulatory T cells in hair follicles and hair follicle stem cell biology.

Thu Jun 01 00:00:00 UTC 2017

Early severe impairment of hematopoietic stem and progenitor cells from the bone marrow caused by CLP sepsis and endotoxemia in a humanized mice model.

Stem cell research & therapy

Skirecki T,Kawiak J,Machaj E,Pojda Z,Wasilewska D,Czubak J,Hoser G

12-3391 was used in Flow cytometry/Cell sorting to elucidate that targeting early hematopoiesis may be considered a viable alternative in the existing arsenal of supportive therapies in sepsis.

Fri Aug 14 00:00:00 UTC 2015

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

12-3391 was used in Flow cytometry/Cell sorting to provide data to show the existence of multi-potent mammary progenitor cells that have pregnancy-specific function.

Mon Dec 08 00:00:00 UTC 2014

Resident CD11b(+)Ly6C(-) lung dendritic cells are responsible for allergic airway sensitization to house dust mite in mice.

PloS one

Mesnil C,Sabatel CM,Marichal T,Toussaint M,Cataldo D,Drion PV,Lekeux P,Bureau F,Desmet CJ

12-3391 was used in Flow cytometry/Cell sorting to determine whether specific conventional dendritic cell subsets are involved in allergic airway sensitisation.

Mon Jun 24 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CD339; jagged canonical Notch ligand 1; Jagged1; Protein jagged-1; Serrate-1

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Gene Aliases: ABE2; Gsfabe2; Htu; Jag1; Ozz; Ser-1

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UniProt ID: (Mouse) Q9QXX0, (Rat) Q63722

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Entrez Gene ID: (Mouse) 16449, (Rat) 29146

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Function(s)

Notch binding calcium ion binding protein binding

Process(es)

blood vessel remodeling morphogenesis of an epithelial sheet T cell mediated immunity pulmonary valve morphogenesis cardiac right ventricle morphogenesis cell communication Notch signaling pathway multicellular organism development organ morphogenesis response to muramyl dipeptide aorta morphogenesis regulation of cell proliferation auditory receptor cell differentiation camera-type eye development negative regulation of cell differentiation negative regulation of fat cell differentiation positive regulation of myeloid cell differentiation negative regulation of neuron differentiation positive regulation of osteoblast differentiation positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter inner ear development cardiac septum morphogenesis ciliary body morphogenesis pulmonary artery morphogenesis cardiac neural crest cell development involved in outflow tract morphogenesis Notch signaling involved in heart development endocardial cushion cell development negative regulation of stem cell differentiation cell fate determination organ regeneration distal tubule development loop of Henle development neuronal stem cell population maintenance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD339 (Jagged 1) Monoclonal Antibody (HMJ1-29), PE, eBioscience™

CD339 (Jagged 1) Antibody (12-3391-82) in Flow

Product Details

12-3391-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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