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          • Primary Antibodies ›
          • CD34 Antibodies

          Invitrogen

          CD34 Monoclonal Antibody (QBEND/10)

          Advanced Verification
          21 Published Figures
          180 References
          View all (163) CD34 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD34 Monoclonal Antibody (QBEND/10)

          • Antibody Testing Data (7)
          • Published Figures (21)
          • Advanced Verification (1)
          CD34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          CD34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 29

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          CD34 Antibody (MA1-10202) in IHC (P)

          Immunohistochemical analysis of CD34 was performed using formalin-fixed paraffin-embedded human small intestine - GIST tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD34 Antibody in Western Blot (WB)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody

          Product Details

          MA1-10202

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 155 publications 155 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          2-8 µg/mL
          View 4 publications 4 publications

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          -
          View 13 publications 13 publications

          Flow Cytometry (Flow)

          5 µg/mL
          View 6 publications 6 publications

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Functional Assay (Functional)

          Assay-dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Non-human primate

          Published species

          Bovine, Human, Mouse, Non-human primate, Rabbit, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          QBEND/10

          Immunogen

          Human endothelial vesicles
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • FITC
          • PE 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          4°C, do not freeze

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11156010

          Product Specific Information

          This antibody reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.

          This antibody will not cross-react with rat, bovine, canine or sheep.

          Functional Application: Induction.

          Target Information

          CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

          View more View less

          Gene Aliases: CD34

          View more View less

          UniProt ID: (Human) P28906

          View more View less

          Entrez Gene ID: (Human) 947

          View more View less

          Function(s)
          transcription factor binding carbohydrate binding sulfate binding
          Process(es)
          tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis
          It has to be done as per old AB suggested Products section.
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