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          • Primary Antibodies ›
          • CD34 Antibodies

          Invitrogen

          CD34 Monoclonal Antibody (RAM34), Alexa Fluor™ 700, eBioscience™

          85 Published Figures
          115 References
          View all (163) CD34 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD34 Monoclonal Antibody (RAM34), Alexa Fluor™ 700, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (85)
          CD34 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD34 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 86

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          CD34 Antibody (56-0341-82) in Flow

          Staining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage eFluor® 450 Cocktail (Product # 88-7772-72), Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82) and 1.0 µg of Anti-Mouse CD34 Alexa Fluor® 700. Lineage negative/low, viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD34 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD34 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD34 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD34 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD34 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD34 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunocytochemistry (ICC/IF)
          CD34 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD34 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Immunohistochemistry (IHC)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)
          CD34 Antibody in Flow Cytometry (Flow)

          Product Details

          56-0341-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 19 publications 19 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 4 publications 4 publications

          Immunocytochemistry (ICC/IF)

          -
          View 10 publications 10 publications

          Flow Cytometry (Flow)

          1 µg/test
          View 82 publications 82 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Goat, Hamster, Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          RAM34

          Conjugate

          Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Biotin 
          • eFluor 450
          • eFluor 660
          • FITC
          • Request custom conjugation

          Excitation/Emission Max

          696/719 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_493998

          Product Specific Information

          Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.

          Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.

          For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.

          The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

          Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD34; cluster designation 34; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

          View more View less

          Gene Aliases: AU040960; Cd34

          View more View less

          UniProt ID: (Mouse) Q64314

          View more View less

          Entrez Gene ID: (Mouse) 12490

          View more View less

          Function(s)
          transcription factor binding carbohydrate binding sulfate binding
          Process(es)
          tissue homeostasis endothelial cell proliferation cell adhesion regulation of blood pressure negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis leukocyte migration hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis
          It has to be done as per old AB suggested Products section.
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