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CD35 Antibody (MA5-17714) in Flow
Flow cytometric analysis of Human Peripheral Blood Leukocytes stained using anti-CD35/CR1 Monoclonal Antibody (Product # MA5-17714) at a 0.5 µg/10^6 cells dilution. Primary antibody was visualization with a secondary antibody - Goat Anti-Mouse FITC at a 1:500 dilution.
Product Details
MA5-17714
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
To5
Immunogen
Mouse monoclonal antibodies raised against human C3b receptor-enriched precipitates from tonsil cell membranes.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein G
Storage buffer
PBS with 4-5mg/mL BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539104
Product Specific Information
MA5-17714 targets CD35/CR1 in FACS applications and shows reactivity with Human samples.
The MA5-17714 immunogen is mouse monoclonal antibodies raised against human C3b receptor-enriched precipitates from tonsil cell membranes.
Target Information
CD35 (CR1, Complement Receptor 1) is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. CD35 protein mediates cellular binding to particles and immune complexes that have activated complement. The CD35 complex is four different allotypes (C,A,B,D) C is 160 kDa; A is 190 kDa; B is 220 kDa and D is 250 kDa. CD35 is involved in the processing of immune complexes, promoting of binding and phagocytosis of C3b complexes and inhibition of complement activation. CD35 is on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukemias, follicular dendritic reticulum cells and is negative on basophils. Decreases in expression of CD35 protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in the CD35 gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants encoding different isoforms of CD35 have been characterized. An additional secreted form of CD35 have also been described but has not been fully characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C3 binding protein; C3-binding protein; C3b/C4b receptor; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); Complement Component (3b/4b) receptor 1 including Knops blood group system; Complement receptor type 1; Knops blood group antigen
Gene Aliases: C3BR; C4BR; CD35; CR1; KN
UniProt ID: (Human) P17927
Entrez Gene ID: (Human) 1378
complement receptor mediated signaling pathway
complement activation, classical pathway
regulation of complement activation
innate immune response
negative regulation of complement activation, alternative pathway
negative regulation of complement activation, classical pathway
viral entry into host cell
negative regulation of serine-type endopeptidase activity
positive regulation of serine-type endopeptidase activity
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