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Invitrogen
CD355 (CRTAM) Monoclonal Antibody (Cr24.1), Alexa Fluor™ 488, eBioscience™
FIGURE: 1 / 1
CD355 (CRTAM) Antibody (53-3559-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 4 hours with the Cell Stimulation Cocktail (Product # 00-4970-93) (right). Cells were then stained with CD8 Monoclonal Antibody, APC (Product # 17-0088-42) and CD355 Monoclonal Antibody, Alexa Fluor 488. Cells in the lymphocyte gate were used for analysis.
Product Details
53-3559-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Cr24.1
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815214
Product Specific Information
Description: This monoclonal antibody Cr24.1 reacts with human CD355, also known as Class-I MHC-restricted T-cell-associated molecule (CRTAM). CD355 usually exists as a homodimer, however, it forms a heterodimer with its ligand Necl-2. CD355 is transiently expressed by activated NK, NKT, gamma delta T, and CD8+ T cells. CD355 is also expressed by Purkinje neurons. Human CD355 is involved in immunosurveillance of cancer cells, gamma delta T cell survival, cell polarity, retention of lymphocytes in lymph nodes, promotion of cytotoxicity in NK cells, and IFN gamma secretion from CD8+ T cells. Cr24.1 has been reported to have antagonist activity.
Applications Reported: This CR24.1 antibody has been reported for use by flow cytometric analysis.
Applications Tested: This CR24.1 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
The CRTAM gene is upregulated in CD4-positive and CD8-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD355; CD355 antigen; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; Class-I MHC-restricted T-cell-associated molecule; cytotoxic and regulatory T cell molecule; Cytotoxic and regulatory T-cell molecule
Gene Aliases: CD355; CRTAM
UniProt ID: (Human) Q59EI1
Entrez Gene ID: (Human) 56253
T cell mediated cytotoxicity
detection of tumor cell
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
cell recognition
positive regulation of natural killer cell mediated cytotoxicity
positive regulation of cytokine secretion
regulation of immune response
activated T cell proliferation
detection of stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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