Invitrogen

CD36 Monoclonal Antibody (HM36), APC, eBioscience™

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1 Published Figure
2 References
View all (91) CD36 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

17-0362-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HM36

Immunogen

CHO cells transfected with full-length mouse CD36
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734967

Product Specific Information

Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII.

Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

Target Information

CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD36; fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; PAS IV; PAS-4; Platelet glycoprotein 4; Platelet glycoprotein IV; SR-B3

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Gene Aliases: Cd36; FAT; GPIV; Scarb3

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UniProt ID: (Mouse) Q08857

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Entrez Gene ID: (Mouse) 12491

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Function(s)
low-density lipoprotein receptor activity protein binding high-density lipoprotein particle binding lipid binding low-density lipoprotein particle binding transforming growth factor beta binding thrombospondin receptor activity oleic acid binding lipoteichoic acid receptor activity lipoprotein particle binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter long-chain fatty acid metabolic process positive regulation of cell-matrix adhesion pattern recognition receptor signaling pathway negative regulation of systemic arterial blood pressure fatty acid metabolic process triglyceride metabolic process transport receptor-mediated endocytosis phagocytosis, recognition cell adhesion signal transduction cell surface receptor signaling pathway nitric oxide mediated signal transduction response to mechanical stimulus negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of cholesterol storage fatty acid transport long-chain fatty acid transport plasma membrane long-chain fatty acid transport negative regulation of angiogenesis fatty acid oxidation lipid storage cGMP-mediated signaling positive regulation of blood coagulation cholesterol transport positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production plasma lipoprotein particle clearance low-density lipoprotein particle clearance lipoprotein transport negative regulation of transcription factor import into nucleus positive regulation of I-kappaB kinase/NF-kappaB signaling apoptotic cell clearance positive regulation of MAPK cascade regulation of transcription from RNA polymerase II promoter in response to oxidative stress negative regulation of growth of symbiont in host long-chain fatty acid import positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-positive bacterium low-density lipoprotein particle mediated signaling positive regulation of phagocytosis, engulfment positive regulation of macrophage cytokine production cellular response to bacterial lipopeptide cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to hydroperoxide regulation of removal of superoxide radicals positive regulation of blood microparticle formation positive regulation of reactive oxygen species metabolic process
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