Invitrogen

CD363 (S1PR1) Monoclonal Antibody (SW4GYPP), eFluor™ 660, eBioscience™

CD363 (S1PR1) Antibody in Flow Cytometry (Flow)

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CD363 (S1PR1) Antibody (50-3639-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0047-42) and Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human CD363 (S1PR1) eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

50-3639-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SW4GYPP

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574208

Product Specific Information

Description: The SW4GYPP monoclonal antibody recognizes human CD363, also known as sphingosine-1-phosphate receptor 1 (S1PR1, S1P1), or endothelial differentiation G-protein coupled receptor 1 (EDG1). CD363 is a part of a family of five G-protein-coupled receptors that bind sphingosine-1-phosphate (S1P), a lysophospholipid mediator. It has been shown to be primarily expressed by lymphocytes but is also seen on endothelial cells, B cells, NK cells, monocytes/macrophages, neural progenitors, neurons, astrocytes, and oligodendrocytes. CD363 surface expression is dependent on the extracellular concentration of S1P and its signaling is important for lymphocyte egress from secondary lymphoid tissues and the thymus. CD363 plays a key role in many cellular processes like angiogenesis, vascular maturation, endothelial barrier function and trafficking of immune cells. Therapeutic CD363 modulators have been successful in the treatment of relapsing-remitting multiple sclerosis through sequestration of immune cells in secondary lymphoid organs and, therefore, away from sites of inflammation.

Applications Reported: This SW4GYPP antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SW4GYPP antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In Vitro Characterization of Sphingosine 1-Phosphate Receptor 1 (S1P₁) Expression and Mediated Migration of Primary Human T and B Cells in the Context of Cenerimod, a Novel, Selective S1P₁ Receptor Modulator.

International journal of molecular sciences

Schlicher L,Kulig P,von Münchow A,Murphy MJ,Keller MP

50-3639-42 was used in Flow cytometry/Cell sorting to show that cenerimod effectively prevents T and B cell migration in a concentration-dependent manner.

Fri Jan 21 00:00:00 UTC 2022

Heterozygous premature termination in zinc-finger domain of Krüppel-like factor 2 gene associates with dysregulated immunity.

Frontiers in immunology

Pernaa N,Keskitalo S,Chowdhury I,Nissinen A,Glumoff V,Keski-Filppula R,Junttila J,Eklund KK,Santaniemi W,Siitonen S,Seppänen MR,Vähäsalo P,Varjosalo M,Åström P,Hautala T

50-3639-42 was used in Flow Cytometry to conclude that KLF2 variant may be associated with familial immune dysregulation. Although the KLF2 deficient family members in our study suffered from lymphopenia, autoimmunity or malignancy, additional study cohorts are required to confirm our observations.

Tue Dec 06 00:00:00 UTC 2022

Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa.

Nature communications

Cantero-Pérez J,Grau-Expósito J,Serra-Peinado C,Rosero DA,Luque-Ballesteros L,Astorga-Gamaza A,Castellví J,Sanhueza T,Tapia G,Lloveras B,Fernández MA,Prado JG,Solé-Sedeno JM,Tarrats A,Lecumberri C,Mañalich-Barrachina L,Centeno-Mediavilla C,Falcó V,Buzon MJ,Genescà M

50-3639-42 was used in Flow Cytometry to study cervical tissues from aviremic ART- treated HIV-1 infected women, to determine if T cells are a cellular reservoir for HIV.

Fri Oct 18 00:00:00 UTC 2019

Detection of HIV-1-specific gastrointestinal tissue resident CD8⁺ T-cells in chronic infection.

Mucosal immunology

Kiniry BE,Li S,Ganesh A,Hunt PW,Somsouk M,Skinner PJ,Deeks SG,Shacklett BL

Tue May 01 00:00:00 UTC 2018

Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis.

International journal of molecular medicine

Ickrath P,Kleinsasser N,Ding X,Ginzkey C,Beyersdorf N,Hagen R,Kerkau T,Hackenberg S

50-3639 was used in Flow cytometry/Cell sorting to characterise CD69+ sphingosine-1-phosphate receptor 1 tissue resident memory T cells in the polyps of patients with chronic rhinosinusitis with nasal polyps.

Wed Aug 01 00:00:00 UTC 2018

Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.

Cell reports

Simula L,Pacella I,Colamatteo A,Procaccini C,Cancila V,Bordi M,Tregnago C,Corrado M,Pigazzi M,Barnaba V,Tripodo C,Matarese G,Piconese S,Campello S

50-3639 was used in Flow cytometry/Cell sorting to describe the crucial role of the mitochondrial protein Drp1 in T cell development and regulation of lymphocyte immune-surveillance.

Tue Dec 11 00:00:00 UTC 2018

Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.

Journal of immunology (Baltimore, Md. : 1950)

Cuff AO,Robertson FP,Stegmann KA,Pallett LJ,Maini MK,Davidson BR,Male V

50-3639 was used in Flow cytometry/Cell sorting to study the molecular signal that characterises an Eomeshi population of natural killer cells in the human liver that is not present in the blood.

Thu Dec 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: CD antigen CD363; CD363; CD363 antigen; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; S1P receptor 1; S1P receptor Edg-1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1

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Gene Aliases: CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; S1P1; S1PR1

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UniProt ID: (Human) P21453

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Entrez Gene ID: (Human) 1901

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Function(s)

G-protein coupled receptor binding G-protein coupled receptor activity sphingosine-1-phosphate receptor activity sphingolipid binding

Process(es)

angiogenesis blood vessel maturation cardiac muscle tissue growth involved in heart morphogenesis sphingosine-1-phosphate signaling pathway chemotaxis cell adhesion G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway brain development cell migration transmission of nerve impulse lamellipodium assembly regulation of cell adhesion neuron differentiation positive regulation of cell migration regulation of bone mineralization actin cytoskeleton reorganization positive regulation of GTPase activity regulation of bone resorption endothelial cell differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle cell proliferation positive regulation of positive chemotaxis positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of stress fiber assembly heart trabecula morphogenesis T cell migration

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CD363 (S1PR1) Monoclonal Antibody (SW4GYPP), eFluor™ 660, eBioscience™

CD363 (S1PR1) Antibody (50-3639-42) in Flow

Product Details

50-3639-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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