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Invitrogen
CD369 (Clec7a, Dectin-1) Monoclonal Antibody (15E2), PerCP-eFluor™ 710, eBioscience™
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CD369 (Clec7a, Dectin-1) Antibody (46-9856-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human/Non-Human Primate CD369 (Clec7a, Dectin-1) PerCP-eFluor® 710. Cells in the lymphocyte and monocyte gate were used for analysis.
Product Details
46-9856-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
15E2
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573910
Product Specific Information
Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predominantly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation.
The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369.
Applications Reported: This 15E2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 15E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-glucan receptor; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; CD369; DC-associated C-type lectin 1; dectin-1; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1
Gene Aliases: BGR; CANDF4; CD369; CLEC7A; CLECSF12; DECTIN1; SCARE2; UNQ539/PRO1082
UniProt ID: (Human) Q9BXN2
Entrez Gene ID: (Human) 64581
pattern recognition receptor signaling pathway
stimulatory C-type lectin receptor signaling pathway
leukocyte activation involved in immune response
phagocytosis, recognition
inflammatory response
cell recognition
carbohydrate mediated signaling
T cell activation
defense response to protozoan
innate immune response
cellular response to molecule of fungal origin
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