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Invitrogen
CD38 Monoclonal Antibody (14.27), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
CD38 Antibody (50-0380-80) in Flow
Staining of rat splenocytes with Anti-Rat CD3 PE (Product # 12-0030-82) and 0.125 µg of Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or 0.125 µg of Anti-Rat CD38 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
50-0380-80
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
14.27
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574158
Product Specific Information
Description: The monoclonal antibody 14.27 reacts with rat CD38, also known as ADP-ribosyl cyclase. CD38 is a type II transmembrane glycoprotein that is an ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic adenosine diphosphate ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP). cADPR and NAADP are secondary messengers involved in calcium mobilization. CD38 can also act as a receptor for CD31 or agonist monoclonal antibodies. CD38 is expressed by leukocytes, early hematopoietic precursor cells, epithelial cells, astrocytes, some muscle cells, kidney cells, and thyroid cells. CD38 plays a role in the production of pro-inflammatory cytokines, proliferation, cell activation, cell migration, cell adhesion, and protection from apoptosis.
Applications Reported: This 14.27 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 14.27 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD38 is a multifunctional ectoenzyme and type II transmembrane glycoprotein involved in various cellular processes. It catalyzes the conversion of NAD into secondary messengers such as nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR). CD38 plays a crucial role in B cell development, with expression levels fluctuating from high in immature cells to low in intermediate ones, and back to high in mature B cells. It is also present in a variety of tissues and hematopoietic cells, including T cells, NK cells, and monocytes, and is used to phenotype leukemias and monitor HIV-1 progression. The CD34+CD38- population is considered to define the most pluripotent hematopoietic stem cells. In addition to its surface expression, CD38 has been identified in the nucleus, where it may regulate calcium levels. It functions as a multi-catalytic ectoenzyme, serving roles as an ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, and possibly NAD+ glycohydrolase, or as a cell surface receptor. CD38 is involved in the activation, proliferation, and differentiation of mature lymphocytes and mediates apoptosis of myeloid and lymphoid progenitor cells. It also participates in cell adhesion, signal transduction, and calcium signaling. Antibodies to CD38 are valuable for subtyping lymphomas and leukemias, detecting plasma cells (such as in myelomas), and marking activated B and T cells. CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma, and neuroblastoma. Dysfunctions in CD38 are associated with diseases like chronic lymphocytic leukemia and Richter's Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase); CD38 antigen p45; CD38H; Cyclic ADP-ribose hydrolase 1; NAD+ nucleosidase
Gene Aliases: ADPRC 1; Cd38
UniProt ID: (Rat) Q64244
Entrez Gene ID: (Rat) 25668
response to hypoxia
positive regulation of cytosolic calcium ion concentration
female pregnancy
metabolic process
response to hormone
positive regulation of cell growth
positive regulation of B cell proliferation
positive regulation of insulin secretion
response to estradiol
response to retinoic acid
response to progesterone
response to hydroperoxide
response to cytokine
response to drug
negative regulation of apoptotic process
negative regulation of bone resorption
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of vasoconstriction
B cell receptor signaling pathway
long term synaptic depression
response to interleukin-1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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