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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD3 Antibodies

          Invitrogen

          CD3e Monoclonal Antibody (145-2C11), Biotin, eBioscience™

          92 Published Figures
          197 References
          View all (501) CD3 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD3e Monoclonal Antibody (145-2C11), Biotin, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (92)
          CD3e Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD3e Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 93

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          CD3e Antibody (13-0031-82) in Flow

          Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and Anti-Mouse CD3e Biotin followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. Quadrants were set based on the autofluorescence sample. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Western Blot (WB)
          CD3e Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)
          CD3e Antibody in Western Blot (WB)
          CD3e Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          CD3e Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD3e Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunocytochemistry, T-Cell Activation (ICC/IF, TCA)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunocytochemistry (ICC/IF)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)

          Product Details

          13-0031-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 16 publications 16 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          -
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 152 publications 152 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          Functional Assay (Functional)

          -
          View 4 publications 4 publications

          T-Cell Activation (TCA)

          -
          View 5 publications 5 publications

          Affinity Purification (AP)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 6 publications 6 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          145-2C11

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Alexa Fluor 488 
          • APC 
          • APC-eFluor 780
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • FITC
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Red 685
          • NovaFluor Red 700
          • NovaFluor Red 710
          • NovaFluor Yellow 610
          • NovaFluor Yellow 660
          • NovaFluor Yellow 690
          • NovaFluor Yellow 700
          • NovaFluor Yellow 730
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 780
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_466319

          Product Specific Information

          Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

          Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

          View more View less

          Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

          View more View less

          UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

          View more View less

          Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

          View more View less

          Function(s)
          transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding
          Process(es)
          protein complex assembly cell surface receptor signaling pathway T cell activation positive thymic T cell selection positive regulation of T cell anergy response to nutrient T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation lymphocyte activation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of calcium-mediated signaling T cell receptor signaling pathway positive regulation of T cell activation apoptotic signaling pathway establishment or maintenance of cell polarity protein transport regulation of lymphocyte apoptotic process
          It has to be done as per old AB suggested Products section.
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