Invitrogen

CD3e Monoclonal Antibody (145-2C11)

1 Published Figure

24 References

View all (501) CD3 antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

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CD3e Antibody (MA5-17655) in Flow

Flow cytometric analysis of Thymocytes staining using anti-CD3e/TCRE Monoclonal Antibody (Product # MA5-17655) at a 0.5 µg/10^6 cells dilution. The Isotypic control was a Purified Hamster IgG (CLCHM00)

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Product Details

MA5-17655

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

View 16 publications 16 publications

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (Functional)

Assay-dependent

Blocking Assay (BLOCK)

View 2 publications 2 publications

T-Cell Activation (TCA)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

145-2C11

Immunogen

H-2Kb sp

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2539045

Product Specific Information

MA5-17655 targets CD3e/TCRE in FACS, FN, IHC (F), IP, TCA, and WB applications and shows reactivity with Mouse samples.

The MA5-17655 immunogen is h-2Kb sp.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antitumor T-cell function requires CPEB4-mediated adaptation to chronic endoplasmic reticulum stress.

The EMBO journal

Fernández-Alfara M,Sibilio A,Martin J,Tusquets Uxó E,Malumbres M,Alcalde V,Chanes V,Cañellas-Socias A,Palomo-Ponce S,Batlle E,Méndez R

MA5-17655 was used in Flow Cytometry to find that, in effector CD8 T lymphocytes, RNA-binding protein CPEB4 constitutes a new branch of the UPR that allows cells to adapt to sustained ER stress, yet remains decoupled from the terminal UPR.

Tue May 02 00:00:00 UTC 2023

Immune translational control by CPEB4 regulates intestinal inflammation resolution and colorectal cancer development.

iScience

Sibilio A,Suñer C,Fernández-Alfara M,Martín J,Berenguer A,Calon A,Chanes V,Millanes-Romero A,Fernández-Miranda G,Batlle E,Fernández M,Méndez R

MA5-17655 was used in Flow Cytometry to investigate the role of transcriptional regulation, by the RNA-binding protein CPEB4, to intestinal immune homeostasis and its role in inflammatory bowel diseases (IBD) and colorectal cancer (CRC) development.

Fri Feb 18 00:00:00 UTC 2022

Immune translational control by CPEB4 regulates intestinal inflammation resolution and colorectal cancer development.

iScience

Sibilio A,Suñer C,Fernández-Alfara M,Martín J,Berenguer A,Calon A,Chanes V,Millanes-Romero A,Fernández-Miranda G,Batlle E,Fernández M,Méndez R

Fri Feb 18 00:00:00 UTC 2022

Immune translational control by CPEB4 regulates intestinal inflammation resolution and colorectal cancer development.

iScience

Sibilio A,Suñer C,Fernández-Alfara M,Martín J,Berenguer A,Calon A,Chanes V,Millanes-Romero A,Fernández-Miranda G,Batlle E,Fernández M,Méndez R

Fri Feb 18 00:00:00 UTC 2022

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System.

BioMed research international

Choi JH,Kim JY,Yi MH,Kim M,Yong TS

MA5-17655 was used in Flow Cytometry to confirm that A. pegreffii plays an essential role in causing asthma in mouse models and has the potential to cause similar effects in humans.

Wed Jan 12 00:00:00 UTC 2022

Postprandial macrophage-derived IL-1β stimulates insulin, and both synergistically promote glucose disposal and inflammation.

Nature immunology

Dror E,Dalmas E,Meier DT,Wueest S,Thévenet J,Thienel C,Timper K,Nordmann TM,Traub S,Schulze F,Item F,Vallois D,Pattou F,Kerr-Conte J,Lavallard V,Berney T,Thorens B,Konrad D,Böni-Schnetzler M,Donath MY

MA517655 was used in flow cytometry to determine a physiological role for IL-1beta and insulin in the regulation of both metabolism and immunity

Wed Mar 01 00:00:00 UTC 2017

Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells.

The EMBO journal

Guidi N,Sacma M,Ständker L,Soller K,Marka G,Eiwen K,Weiss JM,Kirchhoff F,Weil T,Cancelas JA,Florian MC,Geiger H

MA517655 was used in flow cytometry to examine the contribution of stroma-derived osteopontin for hematopoietic stem cell aging

Mon Apr 03 00:00:00 UTC 2017

Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice.

Cellular and molecular life sciences : CMLS

van Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston A

MA517655 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis

Thu Jun 01 00:00:00 UTC 2017

Diet-Derived Short Chain Fatty Acids Stimulate Intestinal Epithelial Cells To Induce Mucosal Tolerogenic Dendritic Cells.

Journal of immunology (Baltimore, Md. : 1950)

Goverse G,Molenaar R,Macia L,Tan J,Erkelens MN,Konijn T,Knippenberg M,Cook EC,Hanekamp D,Veldhoen M,Hartog A,Roeselers G,Mackay CR,Mebius RE

MA5-17655 was used in flow cytometry to assess the effects of dietary fiber and short chain fatty acids on the intestinal immune system and microbiota

Wed Mar 01 00:00:00 UTC 2017

Glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation.

eLife

Araujo L,Khim P,Mkhikian H,Mortales CL,Demetriou M

MA517655 was used in blocking or activating experiment to assess if aerobic glycolysis and glutaminolysis co-operatively reduce UDP-GlcNAc biosynthesis and N-glycan branching in mouse T cell blasts

Fri Jan 06 00:00:00 UTC 2017

Functional MRI for characterization of renal perfusion impairment and edema formation due to acute kidney injury in different mouse strains.

PloS one

Tewes S,Gueler F,Chen R,Gutberlet M,Jang MS,Meier M,Mengel M,Hartung D,Wacker F,Rong S,Hueper K

MA517655 was used in immunohistochemistry to study acute kidney injury in mice by noninvasive measurement of renal perfusion and tissue edema using functional MRI

Wed Aug 23 00:00:00 UTC 2017

CD40-signalling abrogates induction of RORγt⁺ Treg cells by intestinal CD103⁺ DCs and causes fatal colitis.

Nature communications

Barthels C,Ogrinc A,Steyer V,Meier S,Simon F,Wimmer M,Blutke A,Straub T,Zimber-Strobl U,Lutgens E,Marconi P,Ohnmacht C,Garzetti D,Stecher B,Brocker T

MA517655 was used in flow cytometry to report a CD40-dependent mechanism capable of abrogating inducible T regulatory cell induction by dendritic cells

Thu Mar 09 00:00:00 UTC 2017

A human programmed death-ligand 1-expressing mouse tumor model for evaluating the therapeutic efficacy of anti-human PD-L1 antibodies.

Scientific reports

Huang A,Peng D,Guo H,Ben Y,Zuo X,Wu F,Yang X,Teng F,Li Z,Qian X,Qin FX

MA517655 was used in flow cytometry to study a mouse model of anti-human PD-L1 for developing new cancer treatments.

Thu Feb 16 00:00:00 UTC 2017

Th40 cells (CD4+CD40+ Tcells) drive a more severe form of Experimental Autoimmune Encephalomyelitis than conventional CD4 T cells.

PloS one

Vaitaitis GM,Yussman MG,Waid DM,Wagner DH

MA517655 was used in flow cytometry to find that Th40 cells drive a rapid, more severe experimental autoimmune encephalomyelitis disease course than conventional CD4 T cells

Tue Aug 29 00:00:00 UTC 2017

Increase of Frequency and Modulation of Phenotype of Regulatory T Cells by Atorvastatin Is Associated with Decreased Lung Inflammatory Cell Infiltration in a Murine Model of Acute Allergic Asthma.

Frontiers in immunology

Blanquiceth Y,Rodríguez-Perea AL,Tabares Guevara JH,Correa LA,Sánchez MD,Ramírez-Pineda JR,Velilla PA

MA517655 was used in blocking or activating experiment to analyze the in vivo effect of atorvastatin on regulatory T cells and its association with the inflammatory process in a model of allergic asthma

Wed Nov 20 00:00:00 UTC 2019

Glibenclamide Prevents Diabetes in NOD Mice.

PloS one

Lamprianou S,Gysemans C,Bou Saab J,Pontes H,Mathieu C,Meda P

MA517655 was used in flow cytometry to explore if the anti-diabetic sulphonylurea glibenclamide protects insulin-producing cells against conditions mimicking those expected at the onset of type 1 diabetes

Tue Jul 18 00:00:00 UTC 2017

Innate immunological function of TH2 cells in vivo.

Nature immunology

Guo L,Huang Y,Chen X,Hu-Li J,Urban JF,Paul WE

MA5-17655 was used in flow cytometry to report a role for effector T helper type 2 cells during T cell receptor-independent innate-like immune responses

Thu Oct 01 00:00:00 UTC 2015

DNAM-1 controls NK cell activation via an ITT-like motif.

The Journal of experimental medicine

Zhang Z,Wu N,Lu Y,Davidson D,Colonna M,Veillette A

MA5-17655 was used in flow cytometry to study how DNAM-1 controls NK cell-mediated cytotoxicity and cytokine production

Mon Nov 16 00:00:00 UTC 2015

Spatiotemporal requirements for IRF7 in mediating type I IFN-dependent susceptibility to blood-stage Plasmodium infection.

European journal of immunology

Edwards CL,Best SE,Gun SY,Claser C,James KR,de Oca MM,Sebina I,Rivera Fde L,Amante FH,Hertzog PJ,Engwerda CR,Renia L,Haque A

MA5-17655 was used in flow cytometry to determine the role of interferon regulatory factor 7 in Plasmodium berghei ANKA infection

Thu Jan 01 00:00:00 UTC 2015

Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge.

PloS one

Walker CR,Hautefort I,Dalton JE,Overweg K,Egan CE,Bongaerts RJ,Newton DJ,Cruickshank SM,Andrew EM,Carding SR

MA5-17655 was used in flow cytometry to elucidate mechanisms that regulate antimicrobial protein production by Paneth cells

Tue Sep 16 00:00:00 UTC 2014

Hair loss and defective T- and B-cell function in mice lacking ORAI1.

Molecular and cellular biology

Gwack Y,Srikanth S,Oh-Hora M,Hogan PG,Lamperti ED,Yamashita M,Gelinas C,Neems DS,Sasaki Y,Feske S,Prakriya M,Rajewsky K,Rao A

MA5-17655 was used in blocking or activating experiment to characterize the physiological consequences of ORAI1 deficiency

Mon Sep 01 00:00:00 UTC 2008

Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.

Blood

Borsellino G,Kleinewietfeld M,Di Mitri D,Sternjak A,Diamantini A,Giometto R,Höpner S,Centonze D,Bernardi G,Dell'Acqua ML,Rossini PM,Battistini L,Rötzschke O,Falk K

MA5-17655 was used in flow cytometry to determine the expression of CD39 in mouse and human T cells

Wed Aug 15 00:00:00 UTC 2007

Lack of L-selectin expression by cells transferring diabetes in NOD mice: insights into the mechanisms involved in diabetes prevention by Mel-14 antibody treatment.

European journal of immunology

Lepault F,Gagnerault MC,Faveeuw C,Bazin H,Boitard C

MA5-17655 was used in flow cytometry to assess the role of L-selectin in the pathogenesis of autoimmune diabetes.

Thu Jun 01 00:00:00 UTC 1995

Identification of a monoclonal antibody specific for a murine T3 polypeptide.

Proceedings of the National Academy of Sciences of the United States of America

Leo O,Foo M,Sachs DH,Samelson LE,Bluestone JA

MA5-17655 was used in flow cytometry, immunocytochemistry, and immunoprecipitation to identify T3-epsilon as a cell surface protein involved in the transduction of activation signals

Sun Mar 01 00:00:00 UTC 1987

T cell antigen receptor phosphorylation induced by an anti-receptor antibody.

Journal of immunology (Baltimore, Md. : 1950)

Samelson LE,O'Shea JJ,Luong H,Ross P,Urdahl KB,Klausner RD,Bluestone J

MA5-17655 was used in blocking or activating experiment to elucidate how 145-2C11 treatment results in kinase activation and CD3 phosphorylation

Thu Oct 15 00:00:00 UTC 1987

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

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UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD3e Monoclonal Antibody (145-2C11)

CD3e Antibody (MA5-17655) in Flow

Product Details

MA5-17655

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Blocking Assay (BLOCK)

T-Cell Activation (TCA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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