Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD3e Monoclonal Antibody (500A2), Alexa Fluor™ 647
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
CD3e Antibody (HM3421) in Flow
Scientific reports 2015 -
Figure 1 Representative flow cytometric histographs for CCR2 expression in leukocyte subsets as detected by CCR2 reporter protein RFP. Cell suspensions of the peripheral blood, BM, spleen and cervical LNs from the CCR2 RFP reporter mice (CCR2-RFP +/- ) and age-and sex-matched non-transgenic WT C57BL/6J mice were stained with differential subset-specific mAbs. In flow cytometric analyses, subset identificati... View More
Product Details
HM3421
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Syrian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
500A2
Immunogen
C6VL-BS cell lysate
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
purified
Storage buffer
PBS with BSA
Contains
0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536582
Target Information
The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex
Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz
UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942
Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502
protein complex assembly
cell surface receptor signaling pathway
T cell activation
positive thymic T cell selection
positive regulation of T cell anergy
response to nutrient
T cell costimulation
positive regulation of interferon-gamma production
positive regulation of interleukin-4 production
positive regulation of T cell proliferation
negative thymic T cell selection
positive regulation of interleukin-2 biosynthetic process
negative regulation of smoothened signaling pathway
positive regulation of alpha-beta T cell proliferation
lymphocyte activation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
positive regulation of T cell activation
apoptotic signaling pathway
establishment or maintenance of cell polarity
protein transport
regulation of lymphocyte apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support