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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD3 Antibodies

          Invitrogen

          CD3e Monoclonal Antibody (SP7)

          11 Published Figures
          281 References
          View all (501) CD3 antibodies
          Datasheet
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          Datasheet
          Protocols
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          Cite CD3e Monoclonal Antibody (SP7)

          • Antibody Testing Data (3)
          • Published Figures (11)
          CD3e Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          CD3e Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          CD3e Antibody (MA1-90582) in IHC (P)

          Immunohistochemical analysis of CD3e in formalin-fixed, paraffin-embedded human tonsil tissue using a CD3E monoclonal antibody (Product # MA1-90582). Detection was performed with a peroxidase-conjugate and AEC chromagen. Note cell membrane staining of T cells. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD3e Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD3e Antibody in Western Blot (WB)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD3e Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD3e Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD3e Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)
          CD3e Antibody in Immunohistochemistry (IHC)

          Product Details

          MA1-90582

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 240 publications 240 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:150
          View 27 publications 27 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          1:1,000
          View 3 publications 3 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Baboon, Dog, Human, Mouse, Non-human primate, Pig, Rat, Rhesus monkey

          Host/Isotype

          Rabbit / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SP7

          Immunogen

          synthetic peptide KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon chain.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          PBS, pH 7.5, with 1% BSA

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1956722

          Product Specific Information

          This antibody reacts with the intracytoplasmic portion of CD3 and stains human T cells in both the cortex and medula of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin embedded tissues. For tissue staining, antigen retrieval is suggested by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.



          This antibody is predicted to react with Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Baboon, and Woodchuck.



          Recommended positive controls:



          WB - Recombinant human CD3e protein, Jurkat whole cell lysate, Human, mouse and rat thymus tissue lysate|



          IHC (P) - Pig and rat spleen tissue, human tonsil tissue, mouse epidydimal fat pad and lymph node tissues, rat infarcted heart and spleen tissues



          Flow Cytometry - Human peripheral blood lymphocytes, Jurkat cells



          IHC (F) - Mouse brain tissue

          Target Information

          The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD247; CD247 antigen; CD247 antigen, zeta subunit; CD3 antigen delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma polypeptide; CD3 TCR complex; CD3-epsilon; CD3-zeta/eta; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

          View more View less

          Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; CD247; CD3; CD3-DELTA; Cd3-eta; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3epsilon; CD3G; CD3H; CD3Q; CD3Z; Cd3zeta; Ctg-3; Ctg3; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; Tcrk; TCRZ; TCRzeta

          View more View less

          UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693, (Mouse) P24161, (Mouse) P04235, (Rat) P19377, (Mouse) P22646, (Mouse) P11942, (Rat) Q64159

          View more View less

          Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917, (Rat) 25300, (Mouse) 12503, (Mouse) 12500, (Rat) 25710, (Rat) 315609, (Mouse) 12501, (Mouse) 12502, (Rat) 300678

          View more View less

          Function(s)
          transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding molecular_function signal transducer activity
          Process(es)
          positive regulation of T cell anergy protein complex assembly cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway response to nutrient positive regulation of gene expression negative regulation of gene expression T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation T cell activation regulation of apoptotic process negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response positive regulation of calcium-mediated signaling T cell receptor signaling pathway apoptotic signaling pathway T cell differentiation positive thymic T cell selection positive regulation of transcription from RNA polymerase II promoter T cell differentiation in thymus lymphocyte activation positive regulation of T cell activation establishment or maintenance of cell polarity protein transport regulation of lymphocyte apoptotic process biological_process viral process Fc-gamma receptor signaling pathway involved in phagocytosis regulation of defense response to virus by virus
          It has to be done as per old AB suggested Products section.
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