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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD4 Antibodies

          Invitrogen

          CD4 Monoclonal Antibody (OX35), FITC, eBioscience™

          Advanced Verification
          23 Published Figures
          40 References
          View all (609) CD4 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD4 Monoclonal Antibody (OX35), FITC, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (23)
          • Advanced Verification (1)
          CD4 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD4 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 25

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          CD4 Antibody (11-0040-82) in Flow

          Staining of rat splenocytes with Anti-Rat CD3 PE (Product # 12-0030-82) and 0.125 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or 0.125 µg of Anti-Rat CD4 FITC (right). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody

          Product Details

          11-0040-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          0.25 µg/test
          View 38 publications 38 publications
          Product Specifications

          Species Reactivity

          Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2a, kappa

          Recommended Isotype Control

          Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OX35

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • APC 
          • PE 
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_953577

          Product Specific Information

          Description: The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ "double-positive" and CD4+ "single-positive" thymocytes, and by CD4+ "helper" T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.

          Applications Reported: This OX35 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD4; CD4 antigen; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; W3/25 antigen

          View more View less

          Gene Aliases: Cd4; p55; W3/25

          View more View less

          UniProt ID: (Rat) P05540

          View more View less

          Entrez Gene ID: (Rat) 24932

          View more View less

          Function(s)
          glycoprotein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity
          Process(es)
          cytokine production induction by virus of host cell-cell fusion immune response cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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