Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD4 Antibodies

          Invitrogen

          CD4 Monoclonal Antibody (RM4-5), Alexa Fluor™ 532, eBioscience™

          Advanced Verification
          83 Published Figures
          133 References
          View all (609) CD4 antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD4 Monoclonal Antibody (RM4-5), Alexa Fluor™ 532, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (83)
          • Advanced Verification (1)
          CD4 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD4 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 85

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          CD4 Antibody (58-0042-82) in Flow

          Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control Alexa Fluor® 532 (Product # 58-4321-80) (left) or 0.125 µg of Anti-Mouse CD4 Alexa Fluor® 532 (right). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD4 Antibody in Immunocytochemistry (ICC/IF)
          CD4 Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))
          CD4 Antibody in Immunocytochemistry (ICC/IF)
          CD4 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD4 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Immunohistochemistry (IHC)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody in Flow Cytometry (Flow)
          CD4 Antibody

          Product Details

          58-0042-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 11 publications 11 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 116 publications 116 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 532, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          RM4-5

          Conjugate

          Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532
          • Unconjugated
          • Alexa Fluor 561
          • Alexa Fluor 647 
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • eFluor 506
          • eFluor 570
          • eFluor 615
          • FITC
          • NovaFluor Blue 585
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Blue 760
          • NovaFluor Yellow 590
          • NovaFluor Yellow 610
          • NovaFluor Yellow 660
          • Pacific Orange
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          534/553 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11218891

          Product Specific Information

          Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

          Applications Reported: This RM4-5 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.

          Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser

          Target Information

          The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

          View more View less

          Gene Aliases: Cd4; L3T4; Ly-4

          View more View less

          UniProt ID: (Mouse) P06332

          View more View less

          Entrez Gene ID: (Mouse) 12504

          View more View less

          Function(s)
          glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity
          Process(es)
          cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67c77f6dc7-n6nf2:80/100.66.135.186:80.
          git-commit: 545e792480003146e539d624bd2a21b51d198228
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0