Invitrogen

CD4 Monoclonal Antibody (RM4-5), Qdot™ 605

FIGURE: 1 / 3

CD4 Antibody (Q10092) in Flow

Samples were acquired and analyzed using 405 nm excitation specified band pass emission filter on a BD LSR II flow cytometer (BD Biosciences, San Jose, CA). The black line histograms represent cells stained with anti-human CD4 antibody conjugate, and the gray line represents unstained cells.

Product Image Gallery

CD4 Antibody — Figure 7 Number of Tregs in spleen cell suspensions co-cultured in vitro with OVA digests. The percentage of Tregs in cell suspension isolated from spleens of non-stimulated (naive) BALB/c mice cultured in vitro for 48 hours with undigested (0') and after 20 (20') and 40 minutes (40') peptic digest of OVA (white bars), h-OVA (black bars) or b-OVA (grey bars). The data represent the percentage of CD4+Foxp3+ cells out of all live cells as measured by FACS. Representative data from one out of three independent experiments are shown. Data are represented as mean +- SEM. {TM}

Product Details

Q10092

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Immunogen

Mouse CD4

Conjugate

Qdot™ 605 Qdot™ 605 Qdot™ 605

Excitation/Emission Max

300/603 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10374736

Product Specific Information

Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot™ Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The RM4-5 monoclonal antibody (mAb) reacts with CD4 antigen and can be used in immunostaining for flow cytometry and for immunohistochemistry on acetone-fixed, frozen tissue sections.

Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10092: peak excitation 405 (488) nm/peak emission 605 nm; recommended filter 605/20 nm.

Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Seeing the Confetti Colors in a New Light Utilizing Flow Cytometry and Imaging Flow Cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Hagert CF,Bohn AB,Wittenborn TR,Degn SE

Q10092 was used in Flow cytometry/Cell sorting to analyze a 10-color biallelic Confetti reporter which can be used as a high-throughput method for measuring reporter labeling densities and color distributions over time, and may open the door to new avenues of research relying on similar read-outs, for example, tumor heterogeneity and clonal dynamics.

Sat Aug 01 00:00:00 UTC 2020

Seeing the Confetti Colors in a New Light Utilizing Flow Cytometry and Imaging Flow Cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Hagert CF,Bohn AB,Wittenborn TR,Degn SE

Sat Aug 01 00:00:00 UTC 2020

Defining Genetic Variation in Widely Used Congenic and Backcrossed Mouse Models Reveals Varied Regulation of Genes Important for Immune Responses.

Immunity

Chisolm DA,Cheng W,Colburn SA,Silva-Sanchez A,Meza-Perez S,Randall TD,Weinmann AS

Q10092 was used in Flow cytometry/Cell sorting to present strategies to analyze next-generation sequencing datasets to define genetic variation in B6 congenic and transgenic strains.

Tue Jul 16 00:00:00 UTC 2019

Endothelial nitric oxide synthase limits host immunity to control disseminated Candida albicans infections in mice.

PloS one

Navarathna DH,Lionakis MS,Roberts DD

Q10092 was used in Flow cytometry/Cell sorting to explore the role of nitric oxide synthase in fungal innate immunity.

Fri Mar 13 00:00:00 UTC 2020

Dendritic cells, macrophages, NK and CD8⁺ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B.

Virology journal

Lucinda N,Figueiredo MM,Pessoa NL,Santos BS,Lima GK,Freitas AM,Machado AM,Kroon EG,Antonelli LR,Campos MA

Q10092 was used in Flow cytometry/Cell sorting to provide strong evidence that the responses mediated by DCs, Mo/M?, NK and CD8+ T lymphocytes through IL-1β, iNOS and granzyme B production, respectively, together with the production of type I IFN early in the infection, are crucial to host defense against HSV-1.

Tue Feb 21 00:00:00 UTC 2017

CD47 Promotes Protective Innate and Adaptive Immunity in a Mouse Model of Disseminated Candidiasis.

PloS one

Navarathna DH,Stein EV,Lessey-Morillon EC,Nayak D,Martin-Manso G,Roberts DD

Q10092 was used in flow cytometry to study CD47 knock out mice infected with Candida albicans.

Fri Apr 22 00:00:00 UTC 2016

Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development.

Nature immunology

Au-Yeung BB,Melichar HJ,Ross JO,Cheng DA,Zikherman J,Shokat KM,Robey EA,Weiss A

Q10092 was used in flow cytometry to examine the quantitative and temporal requirements of Zap70's function in thymocyte development

Tue Jul 01 00:00:00 UTC 2014

Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis.

PloS one

Billich A,Baumruker T,Beerli C,Bigaud M,Bruns C,Calzascia T,Isken A,Kinzel B,Loetscher E,Metzler B,Mueller M,Nuesslein-Hildesheim B,Kleylein-Sohn B

Q10092 was used in flow cytometry to review the role of S1P lyase in T cell-mediated disease

Tue Sep 17 00:00:00 UTC 2013

Establishment and characterization of a sustained delayed-type hypersensitivity model with arthritic manifestations in C57BL/6J mice.

Arthritis research & therapy

Atkinson SM,Usher PA,Kvist PH,Markholst H,Haase C,Nansen A

Q10092 was used in flow cytometry to establish and characterize a novel murine model of arthritis

Thu Jun 07 00:00:00 UTC 2012

Heat-induced structural changes affect OVA-antigen processing and reduce allergic response in mouse model of food allergy.

PloS one

Golias J,Schwarzer M,Wallner M,Kverka M,Kozakova H,Srutkova D,Klimesova K,Sotkovsky P,Palova-Jelinkova L,Ferreira F,Tuckova L

Q10092 was used in flow cytometry to determine how thermal processing influences ovalbumin's ability to induce allergic symptoms and immune responses in mouse model of food allergy

Mon Dec 17 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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Gene Aliases: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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Function(s)

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

Process(es)

cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (RM4-5), Qdot™ 605

CD4 Antibody (Q10092) in Flow

Product Details

Q10092

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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