Invitrogen

CD4/CD25 Antibody Cocktail, APC, FITC, eBioscience™

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View all (3) CD4/CD25 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

22-0425-73

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

20 µL/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Cocktail

Type

Antibody

Clone

RPA-T4, BC96

Conjugate

APC , FITC APC , FITC APC , FITC

Form

Liquid

Concentration

20 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2043839

Product Specific Information

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59 kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3.

Applications Reported: This product has been reported for use in flow cytometric analysis.

Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test (containing 1 µg of Anti-Human CD4 (RPA-T4) FITC and 0.125 µg of Anti-Human CD25 (BC96) APC). A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Target Information

The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix. CD25: IL2 receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD25; CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL-2 receptor subunit alpha; IL-2R alpha; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2R subunit alpha; Il2ra antibody; interleukin 2 receptor, alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; sIL 2R; soluble IL 2 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; TAC antigen

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Gene Aliases: CD25; CD4; CD4mut; IDDM10; IL2R; IL2RA; IMD41; p55; TCGFR

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UniProt ID: (Human) P01730, (Human) P01589

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Entrez Gene ID: (Human) 920, (Human) 3559

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Function(s)
virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding interleukin-2 receptor activity Ras guanyl-nucleotide exchange factor activity drug binding interleukin-2 binding
Process(es)
MAPK cascade inflammatory response to antigenic stimulus regulation of T cell tolerance induction apoptotic process activation-induced cell death of T cells inflammatory response immune response cell surface receptor signaling pathway Notch signaling pathway cell proliferation interleukin-2-mediated signaling pathway positive regulation of activated T cell proliferation negative regulation of T cell proliferation positive regulation of GTPase activity positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response cytokine production adaptive immune response induction by virus of host cell-cell fusion signal transduction enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation
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