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Invitrogen
CD40 Monoclonal Antibody (5C3), Super Bright™ 436, eBioscience™
FIGURE: 1 / 6
CD40 Antibody (62-0409-42) in Flow
Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG1 kappa Isotype Control, Super Bright 436 (Product # 62-4714-82) (left) or CD40 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
62-0409-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5C3
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2734983
Product Specific Information
Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.
Applications Reported: This 5C3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5C3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5
Gene Aliases: Bp50; CD40; CDW40; p50; TNFRSF5
UniProt ID: (Human) P25942
Entrez Gene ID: (Human) 958
positive regulation of protein phosphorylation
immune response-regulating cell surface receptor signaling pathway
protein complex assembly
cellular calcium ion homeostasis
inflammatory response
immune response
multicellular organism development
platelet activation
positive regulation of B cell proliferation
response to lipopolysaccharide
positive regulation of interleukin-12 production
tumor necrosis factor-mediated signaling pathway
B cell proliferation
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to protozoan
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAP kinase activity
protein kinase B signaling
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
regulation of immune response
regulation of immunoglobulin secretion
positive regulation of NF-kappaB transcription factor activity
defense response to virus
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
positive regulation of protein kinase C signaling
apoptotic signaling pathway
positive regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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