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Invitrogen
CD40 Monoclonal Antibody (LOB7/6)
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Product Details
MA1-80926
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
LOB7/6
Immunogen
CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of human IgG
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS
Contains
<0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_928288
Product Specific Information
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is human tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD40 antibody, clone LOB7/6 recognizes the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.
Target Information
CD40 is a single-chain glycoprotein and a member of the tumor necrosis factor receptor (TNFR) family, exhibiting significant homology to the Hodgkin's disease-associated antigen, CD30. It is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells, as well as some epithelial cells, carcinomas, and lymphoid dendritic cells. Notably, CD40 is present on all B cells except plasma cells. CD40 plays a crucial role in regulating B cell development and maturation, inducing immunoglobulin isotype-switching, and protecting B cells from surface Ig-induced apoptosis when combined with other signals such as IL-4. It promotes proliferation and is essential for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction between CD40 and its ligand CD154 (gp39) on T cells is vital for T-B cell crosstalk, costimulation, and immune regulation. Adaptor protein TNFR2 interacts with CD40, mediating signal transduction, while the AT-hook transcription factor AKNA is reported to regulate CD40 expression, which may be important for homotypic cell interactions. Additionally, the interaction between CD40 and its ligand is necessary for amyloid-beta-induced microglial activation, suggesting a role in the early pathogenesis of Alzheimer's disease. Two alternatively spliced transcript variants encoding distinct isoforms of CD40 have been identified. Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency, highlighting its importance in immune and inflammatory responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
Gene Aliases: Bp50; CD40; CDW40; p50; TNFRSF5
UniProt ID: (Dog) Q7YRL5, (Human) P25942
Entrez Gene ID: (Dog) 403469, (Human) 958
inflammatory response
immune response
multicellular organism development
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
regulation of apoptotic process
positive regulation of MAPK cascade
apoptotic signaling pathway
positive regulation of protein phosphorylation
immune response-regulating cell surface receptor signaling pathway
protein complex assembly
cellular calcium ion homeostasis
platelet activation
positive regulation of B cell proliferation
positive regulation of interleukin-12 production
B cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to protozoan
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAP kinase activity
protein kinase B signaling
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
regulation of immune response
regulation of immunoglobulin secretion
positive regulation of NF-kappaB transcription factor activity
defense response to virus
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
positive regulation of protein kinase C signaling
positive regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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