Invitrogen

CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™

CD43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 22

CD43 Antibody (14-0439-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human spleen stained with 20 µg/mL Anti-Human CD43 Purified followed by Anti-Mouse IgG Biotin, Streptavidin Alkaline Phosphatase, and Fast Red. Nuclei are counterstained with hematoxylin.

Product Image Gallery

CD43 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of CD43 was observed in K-562, MOLT-4, HEL 92.1.7, U-937 in comparison to HT-29, a negative cell line for CD43, using Anti-CD43 Monoclonal Antibody (Product # 14-0439-82) in Western Blot (DOI: 10.1016/j.yexmp.2011.10.009). {RE}

Product Details

14-0439-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

Flow Cytometry (Flow)

20 µg/mL

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Fish, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

eBio84-3C1 (84-3C1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_763493

Product Specific Information

Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This eBio84-3C1 (84-3C1) antibody has been tested by flow cytometric analysis of human peripheral blood cells and by immunohistochemistry of formalin-fixed paraffin embedded human spleen using low pH antigen retreival at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Fri Mar 19 00:00:00 UTC 2021

Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19.

Cell reports. Medicine

Rodriguez L,Pekkarinen PT,Lakshmikanth T,Tan Z,Consiglio CR,Pou C,Chen Y,Mugabo CH,Nguyen NA,Nowlan K,Strandin T,Levanov L,Mikes J,Wang J,Kantele A,Hepojoki J,Vapalahti O,Heinonen S,Kekäläinen E,Brodin P

14-0439 was used in Flow cytometry/Cell sorting to report on a systems-level blood immunomonitoring study of 37 adult patients diagnosed with COVID-19 and followed with up to 14 blood samples from acute to recovery phases of the disease.

Tue Aug 25 00:00:00 UTC 2020

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Journal of human genetics

Ichisima J,Suzuki NM,Samata B,Awaya T,Takahashi J,Hagiwara M,Nakahata T,Saito MK

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2019

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.

Stem cells translational medicine

Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi T

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Mon Jan 01 00:00:00 UTC 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2017

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.

Biomedical reports

Yoon JY,Lee Y,Yu SL,Yoon HK,Park HY,Joung CI,Park SR,Kwon M,Kang J

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Protective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance.

Arteriosclerosis, thrombosis, and vascular biology

Harmon DB,Srikakulapu P,Kaplan JL,Oldham SN,McSkimming C,Garmey JC,Perry HM,Kirby JL,Prohaska TA,Gonen A,Hallowell P,Schirmer B,Tsimikas S,Taylor AM,Witztum JL,McNamara CA

14-0439 was used in Flow cytometry/Cell sorting to investigate the role of B-cell functions in obesity-induced metabolic dysfunction.

Fri Apr 01 00:00:00 UTC 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Tue Sep 01 00:00:00 UTC 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

Published figure using CD43 monoclonal antibody (Product # 14-0439-82) in Flow Cytometry

Thu Jul 17 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

View more View less

Gene Aliases: CD43; GALGP; GPL115; LSN; SPN

View more View less

UniProt ID: (Human) P16150

View more View less

Entrez Gene ID: (Human) 6693

View more View less

Function(s)

transmembrane signaling receptor activity

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation chemotaxis immune response cellular defense response negative regulation of cell adhesion establishment or maintenance of cell polarity signal transduction cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus leukocyte migration apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™

CD43 Antibody (14-0439-82) in IHC (P)

Product Details

14-0439-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!