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          • Primary Antibodies ›
          • CD43 Antibodies

          Invitrogen

          CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™

          Advanced Verification
          19 Published Figures
          10 References
          View all (111) CD43 antibodies

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          Cite CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™

          • Antibody Testing Data (2)
          • Published Figures (19)
          • Advanced Verification (1)
          CD43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          CD43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 22

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          CD43 Antibody (14-0439-82) in IHC (P)

          Immunohistochemistry of formalin-fixed paraffin embedded human spleen stained with 20 µg/mL Anti-Human CD43 Purified followed by Anti-Mouse IgG Biotin, Streptavidin Alkaline Phosphatase, and Fast Red. Nuclei are counterstained with hematoxylin. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CD43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD43 Antibody in Western Blot (WB)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody in Flow Cytometry (Flow)
          CD43 Antibody

          Product Details

          14-0439-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          20 µg/mL
          -

          Flow Cytometry (Flow)

          20 µg/mL
          View 10 publications 10 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Fish, Human

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          eBio84-3C1 (84-3C1)

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • APC 
          • FITC
          • NovaFluor Blue 510
          • NovaFluor Blue 585
          • NovaFluor Blue 690
          • NovaFluor Yellow 570
          • PE 
          • PE-Cyanine7
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_763493

          Product Specific Information

          Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

          Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

          Applications Tested: This eBio84-3C1 (84-3C1) antibody has been tested by flow cytometric analysis of human peripheral blood cells and by immunohistochemistry of formalin-fixed paraffin embedded human spleen using low pH antigen retreival at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

          View more View less

          Gene Aliases: CD43; GALGP; GPL115; LSN; SPN

          View more View less

          UniProt ID: (Human) P16150

          View more View less

          Entrez Gene ID: (Human) 6693

          View more View less

          Function(s)
          transmembrane signaling receptor activity
          Process(es)
          response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation chemotaxis immune response cellular defense response negative regulation of cell adhesion establishment or maintenance of cell polarity signal transduction cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus leukocyte migration apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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