Invitrogen

CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), APC, eBioscience™

14 Published Figures

9 References

View all (111) CD43 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (14)

FIGURE: 1 / 15

CD43 Antibody (17-0439-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD43 APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0439-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Fish, Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio84-3C1 (84-3C1)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11219080

Product Specific Information

Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood monocytes. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Fri Mar 19 00:00:00 UTC 2021

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Journal of human genetics

Ichisima J,Suzuki NM,Samata B,Awaya T,Takahashi J,Hagiwara M,Nakahata T,Saito MK

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Wed May 01 00:00:00 UTC 2019

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.

Stem cells translational medicine

Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi T

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Mon Jan 01 00:00:00 UTC 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

17-0439 was used in Flow cytometry/Cell sorting to investigate the role of KLF1 in maturation of red blood cells.

Sat Apr 01 00:00:00 UTC 2017

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.

Biomedical reports

Yoon JY,Lee Y,Yu SL,Yoon HK,Park HY,Joung CI,Park SR,Kwon M,Kang J

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

17-0439 was used in Flow cytometry/Cell sorting to investigate whether activation of exogenous HOXB4 promotes the production of more mature, definitive red blood cells.

Mon Aug 01 00:00:00 UTC 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Tue Sep 01 00:00:00 UTC 2015

Rapid Proliferation and Differentiation of a Subset of Circulating IgM Memory B Cells to a CpG/Cytokine Stimulus In Vitro.

PloS one

Vásquez C,Franco MA,Angel J

17-0439 was used in Flow cytometry/Cell sorting to compare the phenotype and in vitro functional response of various immune cells in response to CpG and a cytokine cocktail.

Thu Jun 02 00:00:00 UTC 2016

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

17-0439 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

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Gene Aliases: CD43; GALGP; GPL115; LSN; SPN

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UniProt ID: (Human) P16150

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Entrez Gene ID: (Human) 6693

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Function(s)

transmembrane signaling receptor activity

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation chemotaxis immune response cellular defense response negative regulation of cell adhesion establishment or maintenance of cell polarity signal transduction cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus leukocyte migration apoptotic signaling pathway

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CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), APC, eBioscience™

CD43 Antibody (17-0439-42) in Flow

Product Details

17-0439-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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