Invitrogen

CD44 Monoclonal Antibody (Hermes-1)

CD44 Antibody in Immunocytochemistry (ICC/IF)

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CD44 Antibody (MA4400) in ICC/IF

Immunofluorescence analysis of CD44 was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4 % paraformaldehyde for 10 minutes, permeabilized with 0.1 % Triton™ X-100 for 15 minutes, and blocked with 2 % BSA for 45 minutes at room temperature. The cells were labeled with CD44 Monoclonal Antibody (Hermes-1) (Product # MA4400, 1:100) in 0.1 % BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A48269... View More

CD44 Antibody in Immunohistochemistry (IHC)

CD44 Antibody in Flow Cytometry, Neutralization (Flow, Neu)

Product Image Gallery

CD44 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of CD44 was observed in mouse colon which is reported to be positive and not in mouse liver and mouse skeletal muscle using CD44 Monoclonal Antibody (Hermes-1) (Product # MA4400) in western blot. {RE}

Product Details

MA4400

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:10-1:200

View 7 publications 7 publications

Flow Cytometry (Flow)

Assay-dependent

View 3 publications 3 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

Assay-dependent

View 7 publications 7 publications

Functional Assay (Functional)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Goat, Human, Sheep, Rabbit

Published species

Bovine, Human, Mouse, Rabbit

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

Hermes-1

Immunogen

80 - 95 kDa lymphocyte surface glycoprotein H-CAM (CD44).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_223517

Product Specific Information

MA4400 targets CD44 in flow cytometry, immunohistochemistry, immunoprecipitation, ICC/IF and neutralization applications and shows reactivity with bovine, goat, human, ovine, and rabbit samples.

The MA4400 immunogen is 80 - 95 kDa lymphocyte surface glycoprotein H-CAM (CD44). This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Binding of different hyaluronan to CD44 mediates distinct cell adhesion dynamics under shear flow.

The FEBS journal

Li L,Ding Q,Wu Y,Zheng Z,Zhang X,Zhang M,Long M,Lü S

MA4400 was used in Flow Cytometry to indicate that only CD44-HHA interaction under shear stress of 0.2 dynecm-2 could significantly enhance the clustering of CD44, as well as induce the increase in both CD44 and CD18 expression.

Sun Oct 01 00:00:00 UTC 2023

Binding of different hyaluronan to CD44 mediates distinct cell adhesion dynamics under shear flow.

The FEBS journal

Li L,Ding Q,Wu Y,Zheng Z,Zhang X,Zhang M,Long M,Lü S

Sun Oct 01 00:00:00 UTC 2023

A single-cell atlas of the normal and malformed human brain vasculature.

Science (New York, N.Y.)

Winkler EA,Kim CN,Ross JM,Garcia JH,Gil E,Oh I,Chen LQ,Wu D,Catapano JS,Raygor K,Narsinh K,Kim H,Weinsheimer S,Cooke DL,Walcott BP,Lawton MT,Gupta N,Zlokovic BV,Chang EF,Abla AA,Lim DA,Nowakowski TJ

MA4400 was used in Neutralization experiments to illustrate the interplay between vascular and immune cells that contributes to brain hemorrhage and catalog opportunities for targeting angiogenic and inflammatory programs in vascular malformations.

Fri Mar 04 00:00:00 UTC 2022

Label2label: training a neural network to selectively restore cellular structures in fluorescence microscopy.

Journal of cell science

Kölln LS,Salem O,Valli J,Hansen CG,McConnell G

MA4400 was used in Immunocytochemistry to report a new method that trains a CNN to reduce unspecific signals in immunofluorescence images; we name this method label2label (L2L).

Tue Feb 01 00:00:00 UTC 2022

Label2label: training a neural network to selectively restore cellular structures in fluorescence microscopy.

Journal of cell science

Kölln LS,Salem O,Valli J,Hansen CG,McConnell G

MA4400 was used in Immunocytochemistry to report a new method that trains a CNN to reduce unspecific signals in immunofluorescence images; we name this method label2label (L2L).

Tue Feb 01 00:00:00 UTC 2022

Embryo biosensing by uterine natural killer cells determines endometrial fate decisions at implantation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kong CS,Ordoñez AA,Turner S,Tremaine T,Muter J,Lucas ES,Salisbury E,Vassena R,Tiscornia G,Fouladi-Nashta AA,Hartshorne G,Brosens JJ,Brighton PJ

Published figure using CD44 monoclonal antibody (Product # MA4400) in Immunocytochemistry

Thu Apr 01 00:00:00 UTC 2021

Hyaluronic Acid-Functionalized Nanomicelles Enhance SAHA Efficacy in 3D Endometrial Cancer Models.

Cancers

Edwards K,Yao S,Pisano S,Feltracco V,Brusehafer K,Samanta S,Oommen OP,Gazze SA,Paravati R,Maddison H,Li C,Gonzalez D,Conlan RS,Francis L

MA4400 was used in Immunocytochemistry to demonstrate that targeted nanoparticle delivery of SAHA could provide the basis for improving its efficacy in endometrial cancer.

Tue Aug 10 00:00:00 UTC 2021

Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis.

Cell reports

Ko JH,Kim HJ,Jeong HJ,Lee HJ,Oh JY

MA4400 was used in Functional assay to demonstrate the role of mesenchymal stem and stromal cell and macrophage cross-talk in post-injury tissue homeostasis in mice.

Tue Mar 17 00:00:00 UTC 2020

Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin.

Cell metabolism

Wu MJ,Chen YS,Kim MR,Chang CC,Gampala S,Zhang Y,Wang Y,Chang CY,Yang JY,Chang CJ

MA4400 was used in Immunocytochemistry-immunoflourescence to demonstrate the essential role of mitofusin in stem cell fate determination to mediate EMT-associated stemness.

Tue Apr 02 00:00:00 UTC 2019

ADP-ribosylation factor-like 4A interacts with Robo1 to promote cell migration by regulating Cdc42 activation.

Molecular biology of the cell

Chiang TS,Lin MC,Tsai MC,Chen CH,Jang LT,Lee FS

MA4400 was used in Immunocytochemistry-immunoflourescence to study the mechanism by which the binding of ADP-ribosylation factor-like 4A to Robo1 modulates cell migration.

Tue Jan 01 00:00:00 UTC 2019

Woven collagen biotextiles enable mechanically functional rotator cuff tendon regeneration during repair of segmental tendon defects in vivo.

Journal of biomedical materials research. Part B, Applied biomaterials

Learn GD,McClellan PE,Knapik DM,Cumsky JL,Webster-Wood V,Anderson JM,Gillespie RJ,Akkus O

MA4400 was used in Flow cytometry/Cell sorting to investigate the use of electrochemically aligned collagen (ELAC) threads woven into biotextile scaffolds as grafts to repair critically-sized rotator cuff tendon defects in New Zealand White rabbits.

Thu Aug 01 00:00:00 UTC 2019

Respiratory Syncytial Virus Infection of Human Lung Fibroblasts Induces a Hyaluronan-Enriched Extracellular Matrix That Binds Mast Cells and Enhances Expression of Mast Cell Proteases.

Frontiers in immunology

Reeves SR,Barrow KA,Rich LM,White MP,Shubin NJ,Chan CK,Kang I,Ziegler SF,Piliponsky AM,Wight TN,Debley JS

Published figure using CD44 monoclonal antibody (Product # MA4400) in Neutralization

Wed Nov 18 00:00:00 UTC 2020

Lidocaine inhibits cytoskeletal remodelling and human breast cancer cell migration.

British journal of anaesthesia

D'Agostino G,Saporito A,Cecchinato V,Silvestri Y,Borgeat A,Anselmi L,Uguccioni M

MA4400 was used in Inhibition Assays to investigate whether lidocaine can modulate CXCL12-induced responses in a breast cancer cell line.

Mon Oct 01 00:00:00 UTC 2018

Biological role of site-specific O-glycosylation in cell adhesion activity and phosphorylation of osteopontin.

The Biochemical journal

Oyama M,Kariya Y,Kariya Y,Matsumoto K,Kanno M,Yamaguchi Y,Hashimoto Y

MA4400 was used in an ELISA assay to provide conclusive evidence of a novel regulatory mechanism of cell adhesion activity and phosphorylation of OPN by site-specific O-glycosylation.

Wed May 09 00:00:00 UTC 2018

Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment.

Scientific reports

Tarafder S,Gulko J,Sim KH,Yang J,Cook JL,Lee CH

MA4400 was used in Immunohistochemistry-immunofluorescence to study the seamless healing of avascular meniscus tears induced by connective tissue growth factor and transforming growth factor beta 3.

Fri May 25 00:00:00 UTC 2018

Cancer stem cell and its niche in malignant progression of oral potentially malignant disorders.

Oral oncology

Surendran S,Siddappa G,Mohan A,Hicks W,Jayaprakash V,Mimikos C,Mahri M,Almarzouki F,Morrell K,Ravi R,Govindan S,Sushma CN,Raghavan N,Birur P,Ilayaraja J,Merzianu M,Reid M,Suresh A,Kuriakose MA

MA4400 was used in Immunohistochemistry to show progressive increase in expression levels of CSC and its niche markers with increase in grade of dysplasia as compared to the normal cohort.

Fri Dec 01 00:00:00 UTC 2017

Tuning Hydrogel Properties to Promote the Assembly of Salivary Gland Spheroids in 3D.

ACS biomaterials science & engineering

Ozdemir T,Fowler EW,Liu S,Harrington DA,Witt RL,Farach-Carson MC,Pradhan-Bhatt S,Jia X

Published figure using CD44 monoclonal antibody (Product # MA4400) in Immunocytochemistry

Mon Dec 12 00:00:00 UTC 2016

The metalloprotease-disintegrin ADAM8 contributes to temozolomide chemoresistance and enhanced invasiveness of human glioblastoma cells.

Neuro-oncology

Dong F,Eibach M,Bartsch JW,Dolga AM,Schlomann U,Conrad C,Schieber S,Schilling O,Biniossek ML,Culmsee C,Strik H,Koller G,Carl B,Nimsky C

MA4400 was used in blocking or activating experiment to study the role of MMP and ADAM in the temozolomide-induced recurrence of glioblastoma

Sun Nov 01 00:00:00 UTC 2015

Increased cerebrospinal fluid osteopontin levels and its involvement in macrophage infiltration in neuromyelitis optica.

BBA clinical

Kariya Y,Kariya Y,Saito T,Nishiyama S,Honda T,Tanaka K,Yoshida M,Fujihara K,Hashimoto Y

Published figure using CD44 monoclonal antibody (Product # MA4400) in Neutralization

Mon Jun 01 00:00:00 UTC 2015

Increased cerebrospinal fluid osteopontin levels and its involvement in macrophage infiltration in neuromyelitis optica.

BBA clinical

Kariya Y,Kariya Y,Saito T,Nishiyama S,Honda T,Tanaka K,Yoshida M,Fujihara K,Hashimoto Y

Published figure using CD44 monoclonal antibody (Product # MA4400) in Neutralization

Mon Jun 01 00:00:00 UTC 2015

VCAM-1 and VAP-1 recruit myeloid cells that promote pulmonary metastasis in mice.

Blood

Ferjančič Š,Gil-Bernabé AM,Hill SA,Allen PD,Richardson P,Sparey T,Savory E,McGuffog J,Muschel RJ

MA4400 was used in immunohistochemistry to study metastatic myeloid cell recruitment and the roles and therapeutic potential of VCAM-1 and VAP-1

Thu Apr 18 00:00:00 UTC 2013

EGF AND TGF-alpha motogenic activities are mediated by the EGF receptor via distinct matrix-dependent mechanisms.

Experimental cell research

Ellis IR,Schor AM,Schor SL

MA4400 was used in blocking/activating experiment to study the role of EGF receptor on EGF and TGF alpha mitogenic activities

Thu Feb 15 00:00:00 UTC 2007

Fragmented hyaluronan induces transcriptional up-regulation of the multidrug resistance-1 gene in CD4+ T cells.

The Journal of biological chemistry

Tsujimura S,Saito K,Kohno K,Tanaka Y

MA4400 was used in blocking/activating experiment to study the effect of fragmented hyaluronan on the multidrug resistance-1 gene in CD4+ T cells

Fri Dec 08 00:00:00 UTC 2006

Decreased elastin deposition and high proliferation of fibroblasts from Costello syndrome are related to functional deficiency in the 67-kD elastin-binding protein.

American journal of human genetics

Hinek A,Smith AC,Cutiongco EM,Callahan JW,Gripp KW,Weksberg R

MA4400 was used in immunohistochemistry to assess the effect of elastin deposition in Costello syndrome

Wed Mar 01 00:00:00 UTC 2000

Heterotypic cell-cell adhesion of human mast cells to fibroblasts.

Archives of dermatological research

Trautmann A,Feuerstein B,Ernst N,Bröcker EB,Klein CE

MA4400 was used in blocking/activating experiment to investigate the mechanism for the interaction between HMC-1 mast cells and fibroblasts

Sat Mar 01 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: CD44; CD44 antigen; CD44 molecule; CD44 molecule (Indian blood group); CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44

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Gene Aliases: BOS_15086; CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1

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UniProt ID: (Human) P16070

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Entrez Gene ID: (Human) 960, (Bovine) 281057, (Rabbit) 100301546, (Sheep) 101113884

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Function(s)

cytokine receptor activity protein binding collagen binding hyaluronic acid binding

Process(es)

cell-matrix adhesion single organismal cell-cell adhesion extracellular matrix disassembly extracellular matrix organization hyaluronan catabolic process positive regulation of peptidyl-serine phosphorylation positive regulation of heterotypic cell-cell adhesion negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration cartilage development interferon-gamma-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade monocyte aggregation positive regulation of monocyte aggregation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator cell adhesion

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CD44 Monoclonal Antibody (Hermes-1)

CD44 Antibody (MA4400) in ICC/IF

Product Details

MA4400

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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