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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD44 Antibodies

          Invitrogen

          CD44 Monoclonal Antibody (IM7), PerCP-Cyanine5.5, eBioscience™

          86 Published Figures
          228 References
          View all (259) CD44 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD44 Monoclonal Antibody (IM7), PerCP-Cyanine5.5, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (86)
          CD44 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD44 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 87

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          CD44 Antibody (45-0441-82) in Flow

          Staining of C57BL/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.125 µg of Rat IgG2b K Isotype Control PerCP-Cyanine5-5 (Product # 45-4031-80) (left) or 0.125 µg of Anti-Human/Mouse CD44 PerCP-Cyanine5-5 (right). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD44 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)

          Product Details

          45-0441-82

          Applications
          Tested Dilution
          Publications

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          0.25 µg/test
          View 226 publications 226 publications

          In vitro Assay (IV)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Fruit fly, Human, Mouse, Non-human primate

          Host/Isotype

          Rat / IgG2b, kappa

          Recommended Isotype Control

          Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-Cyanine5.5, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          IM7

          Conjugate

          PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5
          • Unconjugated
          • Alexa Fluor 532 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 737
          • Brilliant Violet 421
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • eFluor 450
          • eFluor 506
          • FITC
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Blue 690
          • NovaFluor Blue 725
          • NovaFluor Blue 760
          • NovaFluor Blue 800
          • NovaFluor Red 700
          • NovaFluor Red 710
          • NovaFluor Red 725
          • NovaFluor Red 755
          • NovaFluor UV 765
          • NovaFluor Violet 690
          • NovaFluor Yellow 570
          • NovaFluor Yellow 610
          • NovaFluor Yellow 660
          • NovaFluor Yellow 755
          • NovaFluor Yellow 810
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          489/679 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_925746

          Product Specific Information

          Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

          Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This IM7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD44; CD44 antigen; CD44 molecule; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44

          View more View less

          Gene Aliases: AU023126; AW121933; AW146109; CD44; CDW44; CSPG8; ECMR-III; HCELL; HERMES; HUTCH-I; IN; LHR; Ly-24; MC56; MDU2; MDU3; MIC4; Pgp-1; Pgp1

          View more View less

          UniProt ID: (Human) P16070, (Mouse) P15379

          View more View less

          Entrez Gene ID: (Human) 960, (Mouse) 12505

          View more View less

          Function(s)
          cytokine receptor activity protein binding collagen binding hyaluronic acid binding hyalurononglucosaminidase activity epidermal growth factor receptor binding protein kinase binding cytokine binding phosphoprotein binding
          Process(es)
          cell-matrix adhesion single organismal cell-cell adhesion extracellular matrix disassembly extracellular matrix organization hyaluronan catabolic process positive regulation of peptidyl-serine phosphorylation positive regulation of heterotypic cell-cell adhesion negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration cartilage development interferon-gamma-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade monocyte aggregation positive regulation of monocyte aggregation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of cell growth branching involved in ureteric bud morphogenesis wound healing involved in inflammatory response positive regulation of adaptive immune response negative regulation of mature B cell apoptotic process cell adhesion positive regulation of gene expression Wnt signaling pathway cell migration neuron projection development positive regulation of neutrophil apoptotic process macrophage fusion branching involved in prostate gland morphogenesis
          It has to be done as per old AB suggested Products section.
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