Invitrogen

CD44 Monoclonal Antibody (YKIX337.8), FITC, eBioscience™

6 Published Figures

11 References

View all (259) CD44 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

FIGURE: 1 / 7

CD44 Antibody (11-5440-42) in Flow

Staining of normal canine peripheral blood cells with Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (blue histogram) or Anti-Canine CD44 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-5440-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Dog

Published species

Dog

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YKIX337.8

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10596956

Product Specific Information

Description: This YKIX337.8 monoclonal antibody reacts with canine CD44, also known as phagocytic glycoprotein-1 (Pgp-1). CD44 is an adhesion molecule that binds hyaluronan, a major component of the extracellular matrix. This cell surface molecule is expressed on leukocytes and epithelial cells, as well as in canine mammary and melanocytic tumors. Furthermore, CD44 expression is induced by T cell activation.

Applications Reported: This YKIX337.8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This YKIX337.8 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Canine Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Attenuate Atopic Dermatitis.

Animals : an open access journal from MDPI

Cho BS,Kim SB,Kim S,Rhee B,Yoon J,Lee JW

Published figure using CD44 monoclonal antibody (Product # 11-5440-42) in Flow Cytometry

Thu Jul 06 00:00:00 UTC 2023

The Stromal Vascular Fraction from Canine Adipose Tissue Contains Mesenchymal Stromal Cell Subpopulations That Show Time-Dependent Adhesion to Cell Culture Plastic Vessels.

Animals : an open access journal from MDPI

Scattini G,Pellegrini M,Severi G,Cagiola M,Pascucci L

11-5440-42 was used in Flow cytometry/Cell sorting to investigate their basic properties to speculate on the possible existence of mesenchymal stromal cells subpopulations.

Mon Mar 27 00:00:00 UTC 2023

Proline-based solution maintains cell viability and stemness of canine adipose-derived mesenchymal stem cells after hypothermic storage.

PloS one

Horcharoensuk P,Yang-En S,Narkwichean A,Rungsiwiwut R

Published figure using CD44 monoclonal antibody (Product # 11-5440-42) in Flow Cytometry

Tue Apr 26 00:00:00 UTC 2022

Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints.

Stem cell research & therapy

Teunissen M,Ahrens NS,Snel L,Narcisi R,Kamali SA,van Osch GJVM,Meij BP,Mastbergen SC,Sivasubramaniyan K,Tryfonidou MA

11-5440-42 was used in Flow cytometry/Cell sorting to show that OA cSM-MPCs may be a less optimal candidate for the cell-based treatment of OA than normal cSM-MPCs.

Mon Sep 05 00:00:00 UTC 2022

3D-culture models as drug-testing platforms in canine lymphoma and their cross talk with lymph node-derived stromal cells.

Journal of veterinary science

An JH,Song WJ,Li Q,Bhang DH,Youn HY

11-5440-42 was used in Flow Cytometry to reveal that understanding the interaction between the tumor microenvironment and TCs is essential in designing experimental approaches to personalized medicine and to predict the effect of drugs.

Sat May 01 00:00:00 UTC 2021

Characterization of canine adipose tissue-derived mesenchymal stem cells immortalized by SV40-T retrovirus for therapeutic use.

Journal of cellular physiology

Ayala-Cuellar AP,Kim CW,Hwang KA,Kang JH,Lee G,Cho J,Choi KC

Published figure using CD44 monoclonal antibody (Product # 11-5440-42) in Flow Cytometry

Sun Sep 01 00:00:00 UTC 2019

Allogeneic multipotent mesenchymal stromal cell sheet transplantation promotes healthy healing of wounds caused by zoledronate and dexamethasone in canine mandibular bones.

Regenerative therapy

Kaibuchi N,Iwata T,Onizuka S,Yano K,Tsumanuma Y,Yamato M,Okano T,Ando T

11-5440 was used in Flow cytometry/Cell sorting to demonstrate that zoledronate and dexamethasome in canine mandibular bones promote healthy healing of wounds through mesenchymal stromal sheet transplantation.

Sat Jun 01 00:00:00 UTC 2019

Multi-color flow cytometry for evaluating age-related changes in memory lymphocyte subsets in dogs.

Developmental and comparative immunology

Withers SS,Moore PF,Chang H,Choi JW,McSorley SJ,Kent MS,Monjazeb AM,Canter RJ,Murphy WJ,Sparger EE,Rebhun RB

11-5440 was used in Flow cytometry/Cell sorting to reveal that aged dogs display features of immunosenescence similar to those reported in other species.

Mon Oct 01 00:00:00 UTC 2018

The Effect of a Histone Deacetylase Inhibitor (AR-42) on Canine Prostate Cancer Growth and Metastasis.

The Prostate

Elshafae SM,Kohart NA,Altstadt LA,Dirksen WP,Rosol TJ

Published figure using CD44 monoclonal antibody (Product # 11-5440-42) in Flow Cytometry

Mon May 01 00:00:00 UTC 2017

The canine epiphyseal-derived mesenchymal stem cells are comparable to bone marrow derived-mesenchymal stem cells.

The Journal of veterinary medical science

Chang YP,Hong HP,Lee YH,Liu IH

11-5440 was used in Flow cytometry/Cell sorting to develop a novel method to isolate mesenchymal stem cells from an alternative canine source.

Sun Mar 01 00:00:00 UTC 2015

Therapeutic effects of CTLA4Ig gene-transduced adipose tissue-derived mesenchymal stem cell transplantation on established autoimmune thyroiditis.

Cell transplantation

Choi EW,Lee JM,Lee HW,Yang J,Youn HY

Published figure using CD44 monoclonal antibody (Product # 11-5440-42) in Flow Cytometry

Thu Oct 06 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: CD44 molecule; ECMR-III; ECMRIII; extracellular matrix receptor-III; GP90 lymphocyte homing/adhesion receptor; hermes antigen; HUTCH-I; hyaluronan receptor; hyaluronate receptor; PGP I; PGP-1; PGP-I; PGPI; phagocytic glycoprotein 1; phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44

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Entrez Gene ID: (Dog) 403939

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Function(s)

hyaluronic acid binding

Process(es)

cell adhesion

Performance Guarantee

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CD44 Monoclonal Antibody (YKIX337.8), FITC, eBioscience™

CD44 Antibody (11-5440-42) in Flow

Product Details

11-5440-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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