Invitrogen

CD44H Monoclonal Antibody (OX49), PE, eBioscience™

5 Published Figures

14 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CD44H Antibody (12-0444-82) in Flow

Staining of rat splenocytes with Anti-Rat MHC Class II APC (Product # 17-0920-82) and 0.03 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or Anti-Rat CD44H PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-0444-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX49

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11149352

Product Specific Information

Description: This OX49 monoclonal antibody recognizes rat CD44H, which is also known as homing-associated cell adhesion molecule (H-CAM) or phagocytic glycoprotein-1 (Pgp-1). This isoform of CD44 is the cell surface receptor for hyaluronate, a component of the extracellular matrix. CD44H is highly expressed on hematopoietic and non-hematopoietic cells, and exists as multiple splice variants. CD44H plays a critical role in lymphocyte activation and homing.

Applications Reported: This OX49 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX49 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

G/ β- TCP composite scaffold material promotes osteogenic differentiation of bone marrow mesenchymal stem cells.

Journal of biomedical materials research. Part B, Applied biomaterials

Su H,Liao Y,Yuan X,Huang J,Chen Y,Zhao B

Published figure using CD44H monoclonal antibody (Product # 12-0444-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2023

E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis.

Bioengineering (Basel, Switzerland)

Shi W,Wu J,Pi Y,Yan X,Hu X,Cheng J,Yu H,Shao Z

12-0444-82 was used in Flow cytometry/Cell sorting to examine differentially expressed genes of BMSCs induced by E7 compared to RGD.

Fri Jun 30 00:00:00 UTC 2023

VEGFA-Enriched Exosomes from Tendon-Derived Stem Cells Facilitate Tenocyte Differentiation, Migration, and Transition to a Fibroblastic Phenotype.

BioMed research international

Xue Z,Chen Z,Wu T,Li R,Chen C,Liu J,Hou H,Zheng X,Wang H

Published figure using CD44H monoclonal antibody (Product # 12-0444-82) in Flow Cytometry

Tue Sep 20 00:00:00 UTC 2022

Exosomes from tendon derived stem cells promote tendon repair through miR-144-3p-regulated tenocyte proliferation and migration.

Stem cell research & therapy

Song K,Jiang T,Pan P,Yao Y,Jiang Q

Published figure using CD44H monoclonal antibody (Product # 12-0444-82) in Flow Cytometry

Wed Feb 23 00:00:00 UTC 2022

Dose-effect relationship and molecular mechanism by which BMSC-derived exosomes promote peripheral nerve regeneration after crush injury.

Stem cell research & therapy

Zhao J,Ding Y,He R,Huang K,Liu L,Jiang C,Liu Z,Wang Y,Yan X,Cao F,Huang X,Peng Y,Ren R,He Y,Cui T,Zhang Q,Zhang X,Liu Q,Li Y,Ma Z,Yi X

12-0444 was used in Flow cytometry/Cell sorting to show that BMSC-derived EXOs can promote the regeneration of peripheral nerves and that the mechanism may involve miRNA-mediated regulation of regeneration-related genes, such as VEGFA.

Tue Aug 18 00:00:00 UTC 2020

Dose-effect relationship and molecular mechanism by which BMSC-derived exosomes promote peripheral nerve regeneration after crush injury.

Stem cell research & therapy

Zhao J,Ding Y,He R,Huang K,Liu L,Jiang C,Liu Z,Wang Y,Yan X,Cao F,Huang X,Peng Y,Ren R,He Y,Cui T,Zhang Q,Zhang X,Liu Q,Li Y,Ma Z,Yi X

Tue Aug 18 00:00:00 UTC 2020

Rats provide a superior model of human stress erythropoiesis.

Experimental hematology

Zhang J,Liu Y,Han X,Mei Y,Yang J,Zhang ZJ,Lu X,Ji P

12-0444 was used in Flow cytometry/Cell sorting to establish rat in vitro and in vivo erythropoiesis models that are more appropriate and superior for the study of human stress erythropoiesis than mouse models.

Tue Oct 01 00:00:00 UTC 2019

Co-culture of the bone and bone marrow: a novel way to obtain mesenchymal stem cells with enhanced osteogenic ability for fracture healing in SD rats.

Journal of orthopaedic surgery and research

Zhu C,Sha M,Jiang H,Lin J,Lin W,Li W,Chen X,Huang G,Ding Z

Published figure using CD44H monoclonal antibody (Product # 12-0444-82) in Flow Cytometry

Tue Sep 03 00:00:00 UTC 2019

Bone marrow mesenchymal stem cell-derived exosome uptake and retrograde transport can occur at peripheral nerve endings.

Artificial cells, nanomedicine, and biotechnology

Ren R,Tan XH,Zhao JH,Zhang QP,Zhang XF,Ma ZJ,Peng YN,Liu QB,Zhang HY,Li YQ,He R,Zhao ZQ,Yi XN

12-0444-82 was used in Flow Cytometry to propose a novel drug delivery approach for treating neuronal diseases.

Sun Dec 01 00:00:00 UTC 2019

Inhibition of C-X-C motif chemokine 10 reduces graft loss mediated by memory CD8⁺ T cells in a rat cardiac re-transplant model.

Experimental and therapeutic medicine

Xu J,Ma T,Deng G,Zhuang J,Li C,Wang S,Dai C,Zhou X,Shan Z,Qi Z

12-0444-82 was used in Flow cytometry/Cell sorting to demonstrate that CXCL10 had a crucial role in cardiac transplantation and re-transplantation, and that treatment with CXCL10 antibodies delays accelerated acute rejection mediated by Tm cells in a rat model of cardiac re-transplantation.

Thu Feb 01 00:00:00 UTC 2018

Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model.

Medical science monitor : international medical journal of experimental and clinical research

Chi L,Huang Y,Mao Y,Wu K,Zhang L,Nan G

12-0444 was used in Flow cytometry/Cell sorting to study the pathway through which adipose-derived mesenchymal stem cells affect brain ischaemic injury.

Mon Jun 11 00:00:00 UTC 2018

Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats.

Experimental and therapeutic medicine

Xu B,Li Y,Deng B,Liu X,Wang L,Zhu QL

12-0444 was used in Flow cytometry/Cell sorting to evaluate the suitability of chitosan hydrogel, an injectable scaffold, to deliver mesenchymal stem cells into infarcted myocardium.

Wed Feb 01 00:00:00 UTC 2017

Adipose tissue-derived stem cells promote the reversion of non-alcoholic fatty liver disease: An in vivo study.

International journal of molecular medicine

Liao N,Pan F,Wang Y,Zheng Y,Xu B,Chen W,Gao Y,Cai Z,Liu X,Liu J

12-0444 was used in Flow cytometry/Cell sorting to demonstrate the hepatoprotective effects of adipose tissue-derived stem cells.

Sun May 01 00:00:00 UTC 2016

Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation.

Molecular medicine reports

Fu X,Tong Z,Li Q,Niu Q,Zhang Z,Tong X,Tong L,Zhang X

12-0444 was used in Flow cytometry/Cell sorting to determine that Schwann-like cells derived from ADSCs can undergo mitotic proliferation.

Mon Aug 01 00:00:00 UTC 2016

Interactions of tumour-derived micro(nano)vesicles with human gastric cancer cells.

Journal of translational medicine

Stec M,Szatanek R,Baj-Krzyworzeka M,Baran J,Zembala M,Barbasz J,Waligórska A,Dobrucki JW,Mytar B,Szczepanik A,Siedlar M,Drabik G,Urbanowicz B,Zembala M

12-0444 was used in Flow cytometry/Cell sorting to investigate the contribution of tumour-derived microvesicles to the enhancement of tumour growth.

Tue Dec 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: CD44; CD44 antigen; Cell surface glycoprotein CD44 (hyaluronate binding protein); ECMR-III; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; HUTCH-I; Hyaluronate receptor; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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Gene Aliases: Cd44; CD44A; METAA; RHAMM

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UniProt ID: (Rat) P26051

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Entrez Gene ID: (Rat) 25406

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Function(s)

hyalurononglucosaminidase activity transmembrane signaling receptor activity cytokine receptor activity epidermal growth factor receptor binding protein binding hyaluronic acid binding protein kinase binding cytokine binding phosphoprotein binding

Process(es)

regulation of cell growth branching involved in ureteric bud morphogenesis blood vessel maturation wound healing involved in inflammatory response negative regulation of mature B cell apoptotic process inflammatory response cell adhesion positive regulation of gene expression response to organic cyclic compound Wnt signaling pathway cell migration hyaluronan catabolic process neuron projection development positive regulation of neutrophil apoptotic process positive regulation of peptidyl-serine phosphorylation response to vitamin A positive regulation of heterotypic cell-cell adhesion macrophage fusion negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation cartilage development branching involved in prostate gland morphogenesis positive regulation of ERK1 and ERK2 cascade monocyte aggregation positive regulation of monocyte aggregation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

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CD44H Monoclonal Antibody (OX49), PE, eBioscience™

CD44H Antibody (12-0444-82) in Flow

Product Details

12-0444-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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