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Invitrogen
CD44std Monoclonal Antibody (SFF-304), eBioscience™
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CD44std Antibody (BMS150) in ICC/IF
Immunofluorescence analysis of CD44 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD44std Monoclonal Antibody (SFF-304), eBioscience™ (Product # BMS150) at 1 µg/mL in 0.1% BSA, incubated at 4 degrees Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Pr... View More
Product Details
BMS150
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, lambda
Class
Monoclonal
Type
Antibody
Clone
SFF-304
Immunogen
Rec. Human CD44std
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10597137
Product Specific Information
Description: BMS150 specifically recognizes human standard CD44, but cannot be used to distinguish between splice variants (exon v3-v10).
CD44 is a cell membrane associated, polymorphic glycoprotein with apparent molecular weights ranging from 85 kDa to 250 kDa. CD44 isoforms participate in a wide variety of cell-cell or cell-matrix interactions including lymphocyte homing, establishment of B and T cell immune responses, tumor metastases formation and inflammation.
Three isoform categories of the CD44 molecule have been identified:
1) an 80-90 kDa isoform, the so-called standard form named CD44std, which is widely distributed on several hematopoietic and nonhemato-poietic cells including all subsets of leukocytes, monocytes, erythrocytes, many types of epithelium, mesenchymal elements like fibroblasts, smooth muscle cells and glial cells of the central nervous system,
2) a medium size category of 110-160 kDa which is weakly expressed on epithelial cells and highly expressed in some carcinomas and
3) a category which includes very large isoforms of 250 kDa covalently modified by the addition of chondroitin sulfate.
These bigger isoforms of CD44 arise by alternative splicing of one or more "variant" exons (v2-v10) into the extracellular part of the 90 kDa constant form molecule. Compared to the standard CD44, all larger isoforms are expressed in a much more restricted fashion, only in a few normal tissues or on the surface of certain tumor cells. Some splice variants of CD44 play important and distinct roles in tumor metastasis.
Applications Tested: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blotting.
Purity: >99%.
Target Information
CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD44; CD44 antigen; CD44 molecule; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44
Gene Aliases: CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1
UniProt ID: (Human) P16070
Entrez Gene ID: (Human) 960
cell-matrix adhesion
single organismal cell-cell adhesion
extracellular matrix disassembly
extracellular matrix organization
hyaluronan catabolic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of heterotypic cell-cell adhesion
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
cellular response to fibroblast growth factor stimulus
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
cartilage development
interferon-gamma-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
positive regulation of monocyte aggregation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
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