Invitrogen

CD44var (v6) Monoclonal Antibody (VFF-18), eBioscience™

4 Published Figures

14 References

View all (19) CD44v6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (4)

CD44var (v6) Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 4

CD44var (v6) Antibody (BMS125) in IHC

Molecular imaging and biology 2013 - Fig. 3 Tumor-specific accumulation of fluorescent tracers in breast cancer. a Representative sections of MDA-MB-231 tumors and MCF10DCIS tumor of mice injected with control IgG or CD44v6 Ab. Hematoxylin and eosin ( HE )-stained sections show the noninvasive character of the MCF10DCIS tumor maintaining the preexisting ductal structures intact, whereas the MDA-MB-231 tumors are invading in the mammary fat pad... View More

CD44var (v6) Antibody in Western Blot (WB)

CD44var (v6) Antibody in Western Blot, Flow Cytometry (WB, Flow)

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Product Details

BMS125

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

VFF-18

Immunogen

Rec. Human CD44var (v6)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10596813

Product Specific Information

Description: Specifically recognizes an epitope encoded by exon v6 on the variant portion of human CD44.

Standard CD44 (CD44s) lacks the entire variable region and is found mainly in cells of lymphohematopoietic origin. The multiple isoforms (designated CD44v; v = variant) contain alternatively spliced exons inserted within the extracellular domain. These isoforms are known as CD44v1, CD44v2, CD44v3, CD44v4, CD44v5, CD44v6, CD44v7, CD44v8, CD44v9, CD44v10, CD44v11.

Applications Tested: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blotting.

Purity: >95%.

Target Information

CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Non-canonical Wnt/Ca2+ signaling is essential to promote self-renewal and proliferation in colon cancer stem cells.

Frontiers in oncology

Sarabia-Sánchez MA,Moreno-Londoño AP,Castañeda-Patlán MC,Alvarado-Ortiz E,Martínez-Morales JC,Robles-Flores M

BMS125 was used in Flow Cytometry to indicate that both types of ligands activate the Wnt/Ca2+ signaling axis to induce/maintain the self-renewal efficiency of CSCs, demonstrating to be essential for the functions of CSC in colon cancer.

Tue Mar 28 00:00:00 UTC 2023

ILC1 drive intestinal epithelial and matrix remodelling.

Nature materials

Jowett GM,Norman MDA,Yu TTL,Rosell Arévalo P,Hoogland D,Lust ST,Read E,Hamrud E,Walters NJ,Niazi U,Chung MWH,Marciano D,Omer OS,Zabinski T,Danovi D,Lord GM,Hilborn J,Evans ND,Dreiss CA,Bozec L,Oommen OP,Lorenz CD,da Silva RMP,Neves JF,Gentleman E

BMS125 was used in Immunocytochemistry-immunoflourescence to develop gut organoid cocultures with type-1 innate lymphoid cells (ILC1) to dissect the impact of their accumulation in inflamed intestines.

Mon Feb 01 00:00:00 UTC 2021

A randomized, placebo-controlled phase 2 study of paclitaxel in combination with reparixin compared to paclitaxel alone as front-line therapy for metastatic triple-negative breast cancer (fRida).

Breast cancer research and treatment

Goldstein LJ,Mansutti M,Levy C,Chang JC,Henry S,Fernandez-Perez I,Prausovà J,Staroslawska E,Viale G,Butler B,McCanna S,Ruffini PA,Wicha MS,Schott AF

BMS125 was used in Immunohistochemistry to report the results of a phase 2 randomized, double-blind study [fRida trial (NCT02370238)] evaluating the safety and efficacy of reparixin plus paclitaxel vs. placebo plus paclitaxel in untreated metastatic triple-negative breast cancer.

Mon Nov 01 00:00:00 UTC 2021

PDX-derived organoids model in vivo drug response and secrete biomarkers.

JCI insight

Huang L,Bockorny B,Paul I,Akshinthala D,Frappart PO,Gandarilla O,Bose A,Sanchez-Gonzalez V,Rouse EE,Lehoux SD,Pandell N,Lim CM,Clohessy JG,Grossman J,Gonzalez R,Del Pino SP,Daaboul G,Sawhney MS,Freedman SD,Kleger A,Cummings RD,Emili A,Muthuswamy LB,Hidalgo M,Muthuswamy SK

BMS125 was used in Western Blotting to show organoid cultures can model in vivo drug responses and be used to discover clinically actionable serologic biomarkers.

Thu Nov 05 00:00:00 UTC 2020

PDX-derived organoids model in vivo drug response and secrete biomarkers.

JCI insight

BMS125 was used in Western Blotting to show organoid cultures can model in vivo drug responses and be used to discover clinically actionable serologic biomarkers.

Thu Nov 05 00:00:00 UTC 2020

CD44 alternative splicing and hnRNP A1 expression are associated with the metastasis of breast cancer.

Oncology reports

Loh TJ,Moon H,Cho S,Jang H,Liu YC,Tai H,Jung DW,Williams DR,Kim HR,Shin MG,Liao DJ,Zhou J,Shi W,Zheng X,Shen H

BMS125 was used in Western Blotting to study the effects of the knockdown of hnRNP A1 on the splicing of CD44 in breast cancer cells.

Tue Sep 01 00:00:00 UTC 2015

The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.

SpringerPlus

Santhana Kumar K,Tripolitsioti D,Ma M,Grählert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M

BMS125 was used in flow cytometry to assess the contribution of c-Met signaling to dissemination of medulloblastoma

Tue Jan 27 00:00:00 UTC 2015

The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.

SpringerPlus

Santhana Kumar K,Tripolitsioti D,Ma M,Grählert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M

BMS125 was used in flow cytometry to assess the contribution of c-Met signaling to dissemination of medulloblastoma

Tue Jan 27 00:00:00 UTC 2015

The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.

SpringerPlus

Santhana Kumar K,Tripolitsioti D,Ma M,Grählert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M

BMS125 was used in flow cytometry to assess the contribution of c-Met signaling to dissemination of medulloblastoma

Tue Jan 27 00:00:00 UTC 2015

The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.

SpringerPlus

Santhana Kumar K,Tripolitsioti D,Ma M,Grählert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M

BMS125 was used in flow cytometry to assess the contribution of c-Met signaling to dissemination of medulloblastoma

Tue Jan 27 00:00:00 UTC 2015

Stem cell CD44v isoforms promote intestinal cancer formation in Apc(min) mice downstream of Wnt signaling.

Oncogene

Zeilstra J,Joosten SP,van Andel H,Tolg C,Berns A,Snoek M,van de Wetering M,Spaargaren M,Clevers H,Pals ST

BMS125 was used in Immunohistochemistry to identify CD44v as component of the ISCs program critical for tumor initiation, and as potential treatment target in CRC.

Thu Jan 30 00:00:00 UTC 2014

CD44v6-targeted T cells mediate potent antitumor effects against acute myeloid leukemia and multiple myeloma.

Blood

Casucci M,Nicolis di Robilant B,Falcone L,Camisa B,Norelli M,Genovese P,Gentner B,Gullotta F,Ponzoni M,Bernardi M,Marcatti M,Saudemont A,Bordignon C,Savoldo B,Ciceri F,Naldini L,Dotti G,Bonini C,Bondanza A

BMS125 was used in Immunohistochemistry to study the effects of silencing CD44 isoform variant 6 on the engraftment of acute myeloid leukaemia and multiple myeloma cells in immunocompromised mice.

Thu Nov 14 00:00:00 UTC 2013

Near-infrared fluorescence molecular imaging of ductal carcinoma in situ with CD44v6-specific antibodies in mice: a preclinical study.

Molecular imaging and biology

Vermeulen JF,van Brussel AS,Adams A,Mali WP,van der Wall E,van Diest PJ,Derksen PW

Published figure using CD44var (v6) monoclonal antibody (Product # BMS125) in Immunohistochemistry

Sat Jun 01 00:00:00 UTC 2013

Differential expression of growth factor receptors and membrane-bound tumor markers for imaging in male and female breast cancer.

PloS one

Vermeulen JF,Kornegoor R,van der Wall E,van der Groep P,van Diest PJ

BMS125 was used in Immunohistochemistry to investigate expression profiles of growth factor receptors and membrane-bound tumour markers in male breast cancer and gynecomastia, in comparison with female breast cancer.

Tue Jul 02 00:00:00 UTC 2013

Immunophenotyping invasive breast cancer: paving the road for molecular imaging.

BMC cancer

Vermeulen JF,van Brussel AS,van der Groep P,Morsink FH,Bult P,van der Wall E,van Diest PJ

Published figure using CD44var (v6) monoclonal antibody (Product # BMS125) in Immunofluorescence

Wed Jun 13 00:00:00 UTC 2012

Role of immunohistochemical markers in predicting progression of dysplasia to advanced neoplasia in patients with ulcerative colitis.

Inflammatory bowel diseases

van Schaik FD,Oldenburg B,Offerhaus GJ,Schipper ME,Vleggaar FP,Siersema PD,van Oijen MG,Ten Kate FJ

BMS125 was used in Immunohistochemistry to suggest a role for p53/AMACR coexpression as a potential marker of neoplastic progression in patients with UC.

Thu Mar 01 00:00:00 UTC 2012

Clinicopathological associations of CD44 mRNA and protein expression in primary breast carcinomas.

Histopathology

Berner HS,Suo Z,Risberg B,Villman K,Karlsson MG,Nesland JM

BMS125 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to examine the occurrence of CD44 isoforms in breast carcinomas and their role in predicting clinical outcome.

Sun Jun 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: AL133330.1; CD44; CD44 antigen; CD44 splice variant; CD44 V6; CD44 var; CD44 variant; CD44v6; CD44var (v6); CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; HUTCHI; Hyaluronate receptor; MGC10468; MUTCH-I; PGP I; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; soluble CD44

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Gene Aliases: CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1

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UniProt ID: (Human) P16070

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Entrez Gene ID: (Human) 960

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Function(s)

cytokine receptor activity protein binding collagen binding hyaluronic acid binding

Process(es)

cell-matrix adhesion single organismal cell-cell adhesion extracellular matrix disassembly extracellular matrix organization hyaluronan catabolic process positive regulation of peptidyl-serine phosphorylation positive regulation of heterotypic cell-cell adhesion negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration cartilage development interferon-gamma-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade monocyte aggregation positive regulation of monocyte aggregation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

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CD44var (v6) Monoclonal Antibody (VFF-18), eBioscience™

CD44var (v6) Antibody (BMS125) in IHC

Product Details

BMS125

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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