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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD45 Antibodies

          Invitrogen

          CD45 Monoclonal Antibody (30-F11), eFluor™ 506, eBioscience™

          Advanced Verification
          92 Published Figures
          256 References
          View all (405) CD45 antibodies

          Promotion

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD45 Monoclonal Antibody (30-F11), eFluor™ 506, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (92)
          • Advanced Verification (1)
          CD45 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD45 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 94

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          CD45 Antibody (69-0451-82) in Flow

          Staining of C57Bl/6 bone marrow cells with Anti-Mouse TER-119 PE (Product # 12-5921-82) and 0.25 µg of Rat IgG2b K Isotype Control eFluor® 506 (Product # 69-4031-82) (left) or 0.25 µg of Anti-Mouse CD45 eFluor® 506 (right). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD45 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD45 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD45 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody

          Product Details

          69-0451-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 38 publications 38 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          -
          View 9 publications 9 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 205 publications 205 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rat / IgG2b, kappa

          Recommended Isotype Control

          Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 506, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          30-F11

          Conjugate

          eFluor™ 506 eFluor™ 506 eFluor™ 506
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 561
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • FITC
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 555
          • NovaFluor Blue 585
          • NovaFluor Blue 610-30S
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Blue 660-40S
          • NovaFluor Blue 725
          • NovaFluor Blue 760
          • NovaFluor Blue 800
          • NovaFluor Red 700
          • NovaFluor Red 710
          • NovaFluor UV 765
          • NovaFluor Violet 690
          • NovaFluor Violet 800
          • NovaFluor Yellow 610
          • NovaFluor Yellow 660
          • NovaFluor Yellow 690
          • NovaFluor Yellow 700
          • NovaFluor Yellow 730
          • NovaFluor Yellow 810
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          419/508 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2637147

          Product Specific Information

          Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

          Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

          Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200; T220 and B220

          View more View less

          Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

          View more View less

          UniProt ID: (Mouse) P06800

          View more View less

          Entrez Gene ID: (Mouse) 19264

          View more View less

          Function(s)
          phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding
          Process(es)
          activation of MAPK activity negative regulation of T cell mediated cytotoxicity positive regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration leukocyte cell-cell adhesion response to gamma radiation dephosphorylation B cell differentiation T cell differentiation positive regulation of B cell proliferation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion peptidyl-tyrosine dephosphorylation T cell proliferation B cell proliferation positive regulation of T cell proliferation positive regulation of MAPK cascade cell cycle phase transition positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes bone marrow development stem cell development negative regulation of peptidyl-tyrosine phosphorylation T cell receptor signaling pathway B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

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