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          • Primary Antibodies ›
          • CD45 Antibodies

          Invitrogen

          CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 532, eBioscience™

          Advanced Verification
          54 Published Figures
          54 References
          View all (405) CD45 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 532, eBioscience™

          • Antibody Testing Data (3)
          • Published Figures (54)
          • Advanced Verification (1)
          CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 58

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          CD45 Antibody (58-0459-42) in IHC (P)

          Immunohistochemical analysis of CD45 was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Foll... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry (ICC/IF)
          CD45 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD45 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Immunohistochemistry (IHC)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody in Flow Cytometry (Flow)
          CD45 Antibody

          Product Details

          58-0459-42

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          -
          View 7 publications 7 publications

          Flow Cytometry (Flow)

          5 µL (0.06 µg)/test
          View 43 publications 43 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 532, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          HI30

          Conjugate

          Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532
          • Unconjugated
          • Alexa Fluor 561
          • Alexa Fluor 647 
          • Alexa Fluor 660 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • eFluor 450
          • eFluor 506
          • FITC
          • NovaFluor Blue 510
          • NovaFluor Blue 585
          • NovaFluor Blue 690
          • NovaFluor Red 710
          • NovaFluor Red 725
          • NovaFluor Red 755
          • NovaFluor Yellow 590
          • NovaFluor Yellow 660
          • NovaFluor Yellow 730
          • NovaFluor Yellow 755
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          534/553 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11218673

          Product Specific Information

          Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

          Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.

          Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser

          Target Information

          CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

          View more View less

          Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

          View more View less

          UniProt ID: (Human) P08575

          View more View less

          Entrez Gene ID: (Human) 5788

          View more View less

          Function(s)
          protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding
          Process(es)
          negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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